FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2030314612> ?p ?o ?g. }

• W2030314612 endingPage "167" @default.
• W2030314612 startingPage "156" @default.
• W2030314612 abstract "Maturation of the hepatitis C virus (HCV) polyprotein occurs by a series of proteolytic processes catalyzed by host cell proteases and the virally encoded proteases NS2 and NS3. Although several peptidomimetic inhibitors of NS3 protease have been published, only a few small molecule inhibitors have been reported. In an effort to improve screening efficiency by minimizing the spectral interference of various test compounds, a substrate that contains the longer wavelength fluorescence resonance energy transfer (FRET) pair, TAMRA/QSY-7, was devised. For the optimized substrate T-Abu-Q, with sequence Ac-Asp-Glu-Lys(TAMRA)-Glu-Glu-Abu-Ψ(COO)Ala-Ser-Lys(QSY-7)-amide, the kinetic parameters with HCV NS3 protease are Km = 30 μM, kcat = 0.6 s−1, and kcat/Km = 20,100 s−1 M−1. We show that this substrate is suitable for inhibitor analysis and mechanistic studies so long as the substrate concentration is low enough (0.5 μM) to avoid complications from high inner filter effects. The substrate is especially useful with ultra-high-density screening formats, such as microarrayed compound screening technology, because there is less spectral interference from the compounds being tested than with more traditional (EDANS/DABCYL) FRET protease substrates. The merits of the new substrate, as well as potential applications of this FRET pair to other protease substrates, are discussed." @default.
• W2030314612 created "2016-06-24" @default.
• W2030314612 creator A5013538141 @default.
• W2030314612 creator A5019970981 @default.
• W2030314612 creator A5028515287 @default.
• W2030314612 creator A5031450708 @default.
• W2030314612 creator A5037576259 @default.
• W2030314612 creator A5039075379 @default.
• W2030314612 creator A5042085334 @default.
• W2030314612 creator A5064702968 @default.
• W2030314612 creator A5074957787 @default.
• W2030314612 creator A5075001427 @default.
• W2030314612 creator A5081338791 @default.
• W2030314612 creator A5090638822 @default.
• W2030314612 date "2007-09-01" @default.
• W2030314612 modified "2023-09-27" @default.
• W2030314612 title "Longer wavelength fluorescence resonance energy transfer depsipeptide substrates for hepatitis C virus NS3 protease" @default.
• W2030314612 cites W1549345480 @default.
• W2030314612 cites W1574175588 @default.
• W2030314612 cites W1595507615 @default.
• W2030314612 cites W1985861913 @default.
• W2030314612 cites W2006309458 @default.
• W2030314612 cites W2007608341 @default.
• W2030314612 cites W2028692573 @default.
• W2030314612 cites W2036081511 @default.
• W2030314612 cites W2044278888 @default.
• W2030314612 cites W2056931443 @default.
• W2030314612 cites W2065677510 @default.
• W2030314612 cites W2071873660 @default.
• W2030314612 cites W2072083315 @default.
• W2030314612 cites W2074631079 @default.
• W2030314612 cites W2077451485 @default.
• W2030314612 cites W2078269427 @default.
• W2030314612 cites W2096176556 @default.
• W2030314612 cites W2107217435 @default.
• W2030314612 cites W2119867428 @default.
• W2030314612 cites W2124000798 @default.
• W2030314612 cites W2133198971 @default.
• W2030314612 cites W2145130602 @default.
• W2030314612 doi "https://doi.org/10.1016/j.ab.2007.06.020" @default.
• W2030314612 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/17644059" @default.
• W2030314612 hasPublicationYear "2007" @default.
• W2030314612 type Work @default.
• W2030314612 sameAs 2030314612 @default.
• W2030314612 citedByCount "11" @default.
• W2030314612 countsByYear W20303146122013 @default.
• W2030314612 countsByYear W20303146122014 @default.
• W2030314612 countsByYear W20303146122015 @default.
• W2030314612 countsByYear W20303146122018 @default.
• W2030314612 countsByYear W20303146122019 @default.
• W2030314612 countsByYear W20303146122020 @default.
• W2030314612 countsByYear W20303146122022 @default.
• W2030314612 crossrefType "journal-article" @default.
• W2030314612 hasAuthorship W2030314612A5013538141 @default.
• W2030314612 hasAuthorship W2030314612A5019970981 @default.
• W2030314612 hasAuthorship W2030314612A5028515287 @default.
• W2030314612 hasAuthorship W2030314612A5031450708 @default.
• W2030314612 hasAuthorship W2030314612A5037576259 @default.
• W2030314612 hasAuthorship W2030314612A5039075379 @default.
• W2030314612 hasAuthorship W2030314612A5042085334 @default.
• W2030314612 hasAuthorship W2030314612A5064702968 @default.
• W2030314612 hasAuthorship W2030314612A5074957787 @default.
• W2030314612 hasAuthorship W2030314612A5075001427 @default.
• W2030314612 hasAuthorship W2030314612A5081338791 @default.
• W2030314612 hasAuthorship W2030314612A5090638822 @default.
• W2030314612 hasConcept C121332964 @default.
• W2030314612 hasConcept C132379496 @default.
• W2030314612 hasConcept C181199279 @default.
• W2030314612 hasConcept C182220744 @default.
• W2030314612 hasConcept C185592680 @default.
• W2030314612 hasConcept C18903297 @default.
• W2030314612 hasConcept C21951064 @default.
• W2030314612 hasConcept C2776714187 @default.
• W2030314612 hasConcept C2777289219 @default.
• W2030314612 hasConcept C41183919 @default.
• W2030314612 hasConcept C55493867 @default.
• W2030314612 hasConcept C56856141 @default.
• W2030314612 hasConcept C62520636 @default.
• W2030314612 hasConcept C71240020 @default.
• W2030314612 hasConcept C83100098 @default.
• W2030314612 hasConcept C86803240 @default.
• W2030314612 hasConcept C91881484 @default.
• W2030314612 hasConcept C96305047 @default.
• W2030314612 hasConceptScore W2030314612C121332964 @default.
• W2030314612 hasConceptScore W2030314612C132379496 @default.
• W2030314612 hasConceptScore W2030314612C181199279 @default.
• W2030314612 hasConceptScore W2030314612C182220744 @default.
• W2030314612 hasConceptScore W2030314612C185592680 @default.
• W2030314612 hasConceptScore W2030314612C18903297 @default.
• W2030314612 hasConceptScore W2030314612C21951064 @default.
• W2030314612 hasConceptScore W2030314612C2776714187 @default.
• W2030314612 hasConceptScore W2030314612C2777289219 @default.
• W2030314612 hasConceptScore W2030314612C41183919 @default.
• W2030314612 hasConceptScore W2030314612C55493867 @default.
• W2030314612 hasConceptScore W2030314612C56856141 @default.
• W2030314612 hasConceptScore W2030314612C62520636 @default.
• W2030314612 hasConceptScore W2030314612C71240020 @default.
• W2030314612 hasConceptScore W2030314612C83100098 @default.

• next