Matches in SemOpenAlex for { <https://semopenalex.org/work/W2030339997> ?p ?o ?g. }
Showing items 1 to 80 of
80
with 100 items per page.
- W2030339997 endingPage "224" @default.
- W2030339997 startingPage "219" @default.
- W2030339997 abstract "We have developed a system of recipient strains and donor plasmids that allows targeting of DNA sequences to the am locus of Neurospora crassa. A recipient strain was constructed that contains the 3' two-thirds of the bacterial Hygromicin B (Hy) phosphotransferase-encoding gene (hph) in single copy downstream from the am structural gene encoding NADP-specific glutamate dehydrogenase. Plasmids have been constructed that contain am, but with the 5' end of hph downstream from am. Resistance to Hy can occur during transformation only if recombination occurs within the region of overlap of the hph sequences from the transforming DNA and of the chromosomal hph sequences. A lacZα. gene with a multiple cloning site has been inserted into the region between the am and hph sequences to facilitate subcloning into the targeting vector. Additionally, unique sites at the junctions of the vector and targeting DNA allow easy removal of vector sequences prior to transformation. A second recipient strain has been constructed that has had the am structural gene deleted. This strain may be used to provide a double selection for homologous integration. Additionally, this strain facilitates replacement of am with heterologous constructs, including constructs that drive transcription of am with different promoters. The insertion of hph downstream from am has no apparent effect on am expression, and the resistance segregates as a Mendelian factor in crosses. Transformation efficiency is reduced two to three orders of magnitude, but interestingly, nearly all of the resistant strains have only a single copy of the transforming DNA." @default.
- W2030339997 created "2016-06-24" @default.
- W2030339997 creator A5041548237 @default.
- W2030339997 creator A5083083062 @default.
- W2030339997 date "1994-05-01" @default.
- W2030339997 modified "2023-09-24" @default.
- W2030339997 title "A simple and efficient system for targeting DNA to the am locus of Neurospora crassa" @default.
- W2030339997 cites W2044781753 @default.
- W2030339997 cites W2061182967 @default.
- W2030339997 cites W2069144532 @default.
- W2030339997 cites W2133313719 @default.
- W2030339997 cites W2146476388 @default.
- W2030339997 cites W4232545077 @default.
- W2030339997 doi "https://doi.org/10.1016/0378-1119(94)90264-x" @default.
- W2030339997 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8194755" @default.
- W2030339997 hasPublicationYear "1994" @default.
- W2030339997 type Work @default.
- W2030339997 sameAs 2030339997 @default.
- W2030339997 citedByCount "8" @default.
- W2030339997 countsByYear W20303399972013 @default.
- W2030339997 countsByYear W20303399972014 @default.
- W2030339997 crossrefType "journal-article" @default.
- W2030339997 hasAuthorship W2030339997A5041548237 @default.
- W2030339997 hasAuthorship W2030339997A5083083062 @default.
- W2030339997 hasConcept C101762097 @default.
- W2030339997 hasConcept C104317684 @default.
- W2030339997 hasConcept C107618985 @default.
- W2030339997 hasConcept C111829913 @default.
- W2030339997 hasConcept C143065580 @default.
- W2030339997 hasConcept C150194340 @default.
- W2030339997 hasConcept C153911025 @default.
- W2030339997 hasConcept C163005120 @default.
- W2030339997 hasConcept C204241405 @default.
- W2030339997 hasConcept C22744801 @default.
- W2030339997 hasConcept C2776739539 @default.
- W2030339997 hasConcept C40767141 @default.
- W2030339997 hasConcept C54355233 @default.
- W2030339997 hasConcept C552990157 @default.
- W2030339997 hasConcept C84597430 @default.
- W2030339997 hasConcept C86803240 @default.
- W2030339997 hasConcept C92087593 @default.
- W2030339997 hasConceptScore W2030339997C101762097 @default.
- W2030339997 hasConceptScore W2030339997C104317684 @default.
- W2030339997 hasConceptScore W2030339997C107618985 @default.
- W2030339997 hasConceptScore W2030339997C111829913 @default.
- W2030339997 hasConceptScore W2030339997C143065580 @default.
- W2030339997 hasConceptScore W2030339997C150194340 @default.
- W2030339997 hasConceptScore W2030339997C153911025 @default.
- W2030339997 hasConceptScore W2030339997C163005120 @default.
- W2030339997 hasConceptScore W2030339997C204241405 @default.
- W2030339997 hasConceptScore W2030339997C22744801 @default.
- W2030339997 hasConceptScore W2030339997C2776739539 @default.
- W2030339997 hasConceptScore W2030339997C40767141 @default.
- W2030339997 hasConceptScore W2030339997C54355233 @default.
- W2030339997 hasConceptScore W2030339997C552990157 @default.
- W2030339997 hasConceptScore W2030339997C84597430 @default.
- W2030339997 hasConceptScore W2030339997C86803240 @default.
- W2030339997 hasConceptScore W2030339997C92087593 @default.
- W2030339997 hasIssue "2" @default.
- W2030339997 hasLocation W20303399971 @default.
- W2030339997 hasLocation W20303399972 @default.
- W2030339997 hasOpenAccess W2030339997 @default.
- W2030339997 hasPrimaryLocation W20303399971 @default.
- W2030339997 hasRelatedWork W1571771606 @default.
- W2030339997 hasRelatedWork W1592274023 @default.
- W2030339997 hasRelatedWork W2001956530 @default.
- W2030339997 hasRelatedWork W2024149682 @default.
- W2030339997 hasRelatedWork W2053455147 @default.
- W2030339997 hasRelatedWork W2063908784 @default.
- W2030339997 hasRelatedWork W2076917055 @default.
- W2030339997 hasRelatedWork W2134404749 @default.
- W2030339997 hasRelatedWork W2197065469 @default.
- W2030339997 hasRelatedWork W67957794 @default.
- W2030339997 hasVolume "142" @default.
- W2030339997 isParatext "false" @default.
- W2030339997 isRetracted "false" @default.
- W2030339997 magId "2030339997" @default.
- W2030339997 workType "article" @default.