FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2030341777> ?p ?o ?g. }

• W2030341777 endingPage "8089" @default.
• W2030341777 startingPage "8082" @default.
• W2030341777 abstract "ABSTRACT The cytoplasmic tail of bovine leukemia virus (BLV) transmembrane protein gp30 has multiple amino acid motifs that mimic those present in signaling proteins associated with B-cell and T-cell receptors. The proline-rich motif of gp30, PX 2 PX 4–5 P, is analogous to the recognition site of Src homology 3 (SH3) domains of signaling molecules. Using site-directed mutagenesis of an infectious molecular clone of BLV, point mutations were introduced which changed three of the prolines of the motif to alanines. The influence of these mutations on the pathogenicity of BLV was studied in sheep which received either (i) plasmid DNA with provirus containing proline-to-alanine mutations (pppBLV), (ii) plasmid DNA with wild-type provirus (wtBLV), or (iii) transfection reagent alone. Although all of the BLV-injected animals seroconverted at approximately the same time, viral loads at later time points were high in five of five of the wtBLV group and two of five of the pppBLV group but low in three of five of the pppBLV group, as determined by semiquantitative PCR. Viral expression was lower in the pppBLV-transfected sheep, as measured by p24 antigen enzyme-linked immunosorbent assay in cultured cells, and serologic titers were lower. Thirty-one months after transfection, four of four wtBLV-transfected sheep had died of leukemia and lymphoma, and all five of the pppBLV-transfected sheep were clinically healthy and had normal peripheral blood lymphocyte counts. These data indicate that the proline-rich motif of gp30 is not required for viral infectivity but is important for high viral load in vivo, suggesting that SH3-mediated gp30 interactions are critical for viral pathogenesis following infection. Absence of interactions with the proline-rich motif may prevent or delay tumorigenesis in sheep." @default.
• W2030341777 created "2016-06-24" @default.
• W2030341777 creator A5030187169 @default.
• W2030341777 creator A5051818361 @default.
• W2030341777 creator A5052552737 @default.
• W2030341777 creator A5057659929 @default.
• W2030341777 creator A5066385399 @default.
• W2030341777 date "2001-09-01" @default.
• W2030341777 modified "2023-10-16" @default.
• W2030341777 title "Role of the Proline-Rich Motif of Bovine Leukemia Virus Transmembrane Protein gp30 in Viral Load and Pathogenicity in Sheep" @default.
• W2030341777 cites W1500864073 @default.
• W2030341777 cites W1583914933 @default.
• W2030341777 cites W173263281 @default.
• W2030341777 cites W1737894727 @default.
• W2030341777 cites W1767756239 @default.
• W2030341777 cites W1824655215 @default.
• W2030341777 cites W1913862483 @default.
• W2030341777 cites W1923801572 @default.
• W2030341777 cites W1968111677 @default.
• W2030341777 cites W1971571944 @default.
• W2030341777 cites W1975792051 @default.
• W2030341777 cites W1982186905 @default.
• W2030341777 cites W1983481917 @default.
• W2030341777 cites W1990347667 @default.
• W2030341777 cites W2042209841 @default.
• W2030341777 cites W2042322693 @default.
• W2030341777 cites W2046661738 @default.
• W2030341777 cites W2053276546 @default.
• W2030341777 cites W2056988713 @default.
• W2030341777 cites W2065201288 @default.
• W2030341777 cites W2075441584 @default.
• W2030341777 cites W2081778829 @default.
• W2030341777 cites W2085140159 @default.
• W2030341777 cites W2093293884 @default.
• W2030341777 cites W2093768446 @default.
• W2030341777 cites W2104603153 @default.
• W2030341777 cites W2120964363 @default.
• W2030341777 cites W2125922177 @default.
• W2030341777 cites W2146667353 @default.
• W2030341777 cites W2147833851 @default.
• W2030341777 cites W2260664721 @default.
• W2030341777 cites W2403813013 @default.
• W2030341777 doi "https://doi.org/10.1128/jvi.75.17.8082-8089.2001" @default.
• W2030341777 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/115052" @default.
• W2030341777 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11483753" @default.
• W2030341777 hasPublicationYear "2001" @default.
• W2030341777 type Work @default.
• W2030341777 sameAs 2030341777 @default.
• W2030341777 citedByCount "15" @default.
• W2030341777 countsByYear W20303417772012 @default.
• W2030341777 countsByYear W20303417772013 @default.
• W2030341777 countsByYear W20303417772014 @default.
• W2030341777 countsByYear W20303417772017 @default.
• W2030341777 countsByYear W20303417772020 @default.
• W2030341777 crossrefType "journal-article" @default.
• W2030341777 hasAuthorship W2030341777A5030187169 @default.
• W2030341777 hasAuthorship W2030341777A5051818361 @default.
• W2030341777 hasAuthorship W2030341777A5052552737 @default.
• W2030341777 hasAuthorship W2030341777A5057659929 @default.
• W2030341777 hasAuthorship W2030341777A5066385399 @default.
• W2030341777 hasBestOaLocation W20303417771 @default.
• W2030341777 hasConcept C102906071 @default.
• W2030341777 hasConcept C104317684 @default.
• W2030341777 hasConcept C106358424 @default.
• W2030341777 hasConcept C118892022 @default.
• W2030341777 hasConcept C141231307 @default.
• W2030341777 hasConcept C153911025 @default.
• W2030341777 hasConcept C159047783 @default.
• W2030341777 hasConcept C2522874641 @default.
• W2030341777 hasConcept C2778645089 @default.
• W2030341777 hasConcept C2779562191 @default.
• W2030341777 hasConcept C515207424 @default.
• W2030341777 hasConcept C54009773 @default.
• W2030341777 hasConcept C55493867 @default.
• W2030341777 hasConcept C86803240 @default.
• W2030341777 hasConceptScore W2030341777C102906071 @default.
• W2030341777 hasConceptScore W2030341777C104317684 @default.
• W2030341777 hasConceptScore W2030341777C106358424 @default.
• W2030341777 hasConceptScore W2030341777C118892022 @default.
• W2030341777 hasConceptScore W2030341777C141231307 @default.
• W2030341777 hasConceptScore W2030341777C153911025 @default.
• W2030341777 hasConceptScore W2030341777C159047783 @default.
• W2030341777 hasConceptScore W2030341777C2522874641 @default.
• W2030341777 hasConceptScore W2030341777C2778645089 @default.
• W2030341777 hasConceptScore W2030341777C2779562191 @default.
• W2030341777 hasConceptScore W2030341777C515207424 @default.
• W2030341777 hasConceptScore W2030341777C54009773 @default.
• W2030341777 hasConceptScore W2030341777C55493867 @default.
• W2030341777 hasConceptScore W2030341777C86803240 @default.
• W2030341777 hasIssue "17" @default.
• W2030341777 hasLocation W20303417771 @default.
• W2030341777 hasLocation W20303417772 @default.
• W2030341777 hasLocation W20303417773 @default.
• W2030341777 hasLocation W20303417774 @default.
• W2030341777 hasOpenAccess W2030341777 @default.
• W2030341777 hasPrimaryLocation W20303417771 @default.
• W2030341777 hasRelatedWork W1573867046 @default.
• W2030341777 hasRelatedWork W2012849123 @default.

• next