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• W2030362048 abstract "Understanding how human cells in tissue culture adapt to hypothermia may aid in developing new clinical procedures for improved ischemic and hypothermic protection. Human coronary artery endothelial cells grown to confluence at 37°C and then transferred to 25°C become resistant over time to oxidative stress and injury induced by 0°C storage and rewarming. This protection correlates with an increase in intracellular glutathione at 25°C. To help understand the molecular basis of endothelial cold-adaptation, isolated proteins from cold-adapted (25°C/72 h) and pre-adapted cells were analyzed by quantitative proteomic methods and differentially expressed proteins were categorized using the DAVID Bioinformatics Resource. Cells adapted to 25°C expressed changes in the abundance of 219 unique proteins representing a broad range of categories such as translation, glycolysis, biosynthetic (anabolic) processes, NAD, cytoskeletal organization, RNA processing, oxidoreductase activity, response-to-stress and cell redox homeostasis. The number of proteins that decreased significantly with cold-adaptation exceeded the number that increased by 2:1. Almost half of the decreases were associated with protein metabolic processes and a third were related to anabolic processes including protein, DNA and fatty acid synthesis. Changes consistent with the suppression of cytoskeletal dynamics provided further evidence that cold-adapted cells are in an energy conserving state. Among the specific changes were increases in the abundance and activity of redox proteins glutathione S-transferase, thioredoxin and thioredoxin reductase, which correlated with a decrease in oxidative stress, an increase in protein glutathionylation, and a recovery of reduced protein thiols during rewarming from 0°C. Increases in S-adenosylhomocysteine hydrolase and nicotinamide phosphoribosyltransferase implicate a central role for the methionine-cysteine transulfuration pathway in increasing glutathione levels and the NAD salvage pathway in increasing the reducing capacity of cold-adapted cells. Endothelial adaptation to mild-moderate hypothermia down-regulates anabolic processes and increases the reducing capacity of cells to enhance their resistance to oxidation and injury associated with 0°C storage and rewarming. Inducing these characteristics in a clinical setting could potentially limit the damaging effects of energy insufficiency due to ischemia and prevent the disruption of integrated metabolism at low temperatures." @default.
• W2030362048 created "2016-06-24" @default.
• W2030362048 creator A5046210672 @default.
• W2030362048 creator A5048663075 @default.
• W2030362048 creator A5068556015 @default.
• W2030362048 date "2011-12-01" @default.
• W2030362048 modified "2023-10-10" @default.
• W2030362048 title "Proteomic analysis of endothelial cold-adaptation" @default.
• W2030362048 cites W1494755399 @default.
• W2030362048 cites W1500690807 @default.
• W2030362048 cites W1516434804 @default.
• W2030362048 cites W1518531052 @default.
• W2030362048 cites W1524473687 @default.
• W2030362048 cites W1592590408 @default.
• W2030362048 cites W1935061717 @default.
• W2030362048 cites W1965518476 @default.
• W2030362048 cites W1966285165 @default.
• W2030362048 cites W1974869556 @default.
• W2030362048 cites W1976014825 @default.
• W2030362048 cites W1976237986 @default.
• W2030362048 cites W1976626368 @default.
• W2030362048 cites W1976674039 @default.
• W2030362048 cites W1980824787 @default.
• W2030362048 cites W1985272136 @default.
• W2030362048 cites W1985556486 @default.
• W2030362048 cites W1986320836 @default.
• W2030362048 cites W1989383248 @default.
• W2030362048 cites W1992779899 @default.
• W2030362048 cites W1995420512 @default.
• W2030362048 cites W1996826558 @default.
• W2030362048 cites W1998539678 @default.
• W2030362048 cites W2001281440 @default.
• W2030362048 cites W2002067726 @default.
• W2030362048 cites W2003529245 @default.
• W2030362048 cites W2004467675 @default.
• W2030362048 cites W2004963616 @default.
• W2030362048 cites W2005292744 @default.
• W2030362048 cites W2005493046 @default.
• W2030362048 cites W2006806744 @default.
• W2030362048 cites W2007107430 @default.
• W2030362048 cites W2008653692 @default.
• W2030362048 cites W2009262914 @default.
• W2030362048 cites W2009364597 @default.
• W2030362048 cites W2010132718 @default.
• W2030362048 cites W2011783801 @default.
• W2030362048 cites W2014880757 @default.
• W2030362048 cites W2019745044 @default.
• W2030362048 cites W2020902432 @default.
• W2030362048 cites W2022429405 @default.
• W2030362048 cites W2024342524 @default.
• W2030362048 cites W2025944845 @default.
• W2030362048 cites W2027404569 @default.
• W2030362048 cites W2027810531 @default.
• W2030362048 cites W2038556759 @default.
• W2030362048 cites W2039511668 @default.
• W2030362048 cites W2039892294 @default.
• W2030362048 cites W2040005072 @default.
• W2030362048 cites W2041672668 @default.
• W2030362048 cites W2042054309 @default.
• W2030362048 cites W2048291145 @default.
• W2030362048 cites W2049821479 @default.
• W2030362048 cites W2051795211 @default.
• W2030362048 cites W2052538238 @default.
• W2030362048 cites W2052990311 @default.
• W2030362048 cites W2053306150 @default.
• W2030362048 cites W2062918974 @default.
• W2030362048 cites W2068327601 @default.
• W2030362048 cites W2069355104 @default.
• W2030362048 cites W2071012814 @default.
• W2030362048 cites W2071334751 @default.
• W2030362048 cites W2080577763 @default.
• W2030362048 cites W2087043303 @default.
• W2030362048 cites W2087134765 @default.
• W2030362048 cites W2088817869 @default.
• W2030362048 cites W2090355988 @default.
• W2030362048 cites W2092093142 @default.
• W2030362048 cites W2092485098 @default.
• W2030362048 cites W2093393970 @default.
• W2030362048 cites W2095557093 @default.
• W2030362048 cites W2096697607 @default.
• W2030362048 cites W2103017472 @default.
• W2030362048 cites W2103108890 @default.
• W2030362048 cites W2105809801 @default.
• W2030362048 cites W2108536847 @default.
• W2030362048 cites W2110154943 @default.
• W2030362048 cites W2110739636 @default.
• W2030362048 cites W2113695502 @default.
• W2030362048 cites W2115035287 @default.
• W2030362048 cites W2117327695 @default.
• W2030362048 cites W2122683221 @default.
• W2030362048 cites W2123818285 @default.
• W2030362048 cites W2125866590 @default.
• W2030362048 cites W2128196906 @default.
• W2030362048 cites W2131229011 @default.
• W2030362048 cites W2131632095 @default.
• W2030362048 cites W2131865548 @default.
• W2030362048 cites W2136012353 @default.
• W2030362048 cites W2137319242 @default.
• W2030362048 cites W2137417976 @default.
• W2030362048 cites W2141771438 @default.

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