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- W2030370574 endingPage "434" @default.
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- W2030370574 abstract "Annexin A6 (AnxA6), calcium- and membrane-binding protein, is involved in membrane dynamics. It exists in the cell in two isoforms, AnxA6-1 and AnxA6-2, varying only by the VAAEIL sequence. In most cells, AnxA6-1 predominates. A limited number of observations suggests that both isoforms differ from each other functionally. The EGF-dependent Ca(2+) influx in A431 cells is inhibited only by AnxA6-1. Moreover, AnxA6-2 was found to exhibit higher affinity for Ca(2+). In this report we addressed the potential significance of the VAAEIL deletion in AnxA6-2. For this purpose, we expressed AnxA6 isoform cDNAs in bacteria or mouse Balb/3T3 fibroblasts. The recombinant AnxA6-2 was characterized by a less extended molecular shape than that of AnxA6-1 and required a narrower [Ca(2+)] range to bind liposomes. Upon lowering pH in the presence of EGTA recombinant AnxA6-2 became less hydrophobic than AnxA6-1 as revealed by the Triton X-114 partition. Furthermore, AnxA6-2 revealed stronger F-actin binding than that of AnxA6-1. Immunofluorescence microscopy showed that the EGFP-tagged AnxA6 isoforms expressed in Balb/3T3 fibroblasts relocate in a Ca(2+)- and H(+)-sensitive manner to the vesicular structures in a perinuclear region or in cytosol. Cell fractionation showed that in resting conditions AnxA6-1 is associated with early endosomes and AnxA6-2 with late endosomes, and an increase in [Ca(2+)] and/or [H(+)] induced their opposite distribution. These findings suggest a potentially independent regulation, localization, and function of AnxA6 isoforms in Balb/3T3 fibroblasts. More generally, our findings suggest distinct functions of AnxA6 isoforms in membrane dynamics." @default.
- W2030370574 created "2016-06-24" @default.
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- W2030370574 date "2008-01-01" @default.
- W2030370574 modified "2023-10-18" @default.
- W2030370574 title "Calcium- and pH-dependent localization of annexin A6 isoforms in Balb/3T3 fibroblasts reflecting their potential participation in vesicular transport" @default.
- W2030370574 cites W1552779466 @default.
- W2030370574 cites W1555536578 @default.
- W2030370574 cites W1604221810 @default.
- W2030370574 cites W1614056212 @default.
- W2030370574 cites W1715840330 @default.
- W2030370574 cites W176220363 @default.
- W2030370574 cites W1932669647 @default.
- W2030370574 cites W1949772429 @default.
- W2030370574 cites W1980515644 @default.
- W2030370574 cites W1980917046 @default.
- W2030370574 cites W1983202302 @default.
- W2030370574 cites W1984953308 @default.
- W2030370574 cites W1987348213 @default.
- W2030370574 cites W1990852843 @default.
- W2030370574 cites W1992400924 @default.
- W2030370574 cites W1996453362 @default.
- W2030370574 cites W1997934608 @default.
- W2030370574 cites W2002842138 @default.
- W2030370574 cites W2004026262 @default.
- W2030370574 cites W2005574904 @default.
- W2030370574 cites W2005758066 @default.
- W2030370574 cites W2007861835 @default.
- W2030370574 cites W2009776121 @default.
- W2030370574 cites W2011115262 @default.
- W2030370574 cites W2018688415 @default.
- W2030370574 cites W2019031014 @default.
- W2030370574 cites W2022969523 @default.
- W2030370574 cites W2029781087 @default.
- W2030370574 cites W2030506720 @default.
- W2030370574 cites W2037083050 @default.
- W2030370574 cites W2043369560 @default.
- W2030370574 cites W2043653507 @default.
- W2030370574 cites W2046445536 @default.
- W2030370574 cites W2048632014 @default.
- W2030370574 cites W2049707799 @default.
- W2030370574 cites W2053756180 @default.
- W2030370574 cites W2054427537 @default.
- W2030370574 cites W2062461694 @default.
- W2030370574 cites W2065365365 @default.
- W2030370574 cites W2067137414 @default.
- W2030370574 cites W2067391888 @default.
- W2030370574 cites W2070314562 @default.
- W2030370574 cites W2073481159 @default.
- W2030370574 cites W2074893946 @default.
- W2030370574 cites W2076784356 @default.
- W2030370574 cites W2077789771 @default.
- W2030370574 cites W2080382332 @default.
- W2030370574 cites W2080856606 @default.
- W2030370574 cites W2082653871 @default.
- W2030370574 cites W2083773088 @default.
- W2030370574 cites W2084448537 @default.
- W2030370574 cites W2086418977 @default.
- W2030370574 cites W2087181878 @default.
- W2030370574 cites W2088485431 @default.
- W2030370574 cites W2092491511 @default.
- W2030370574 cites W2114828347 @default.
- W2030370574 cites W2115500071 @default.
- W2030370574 cites W2115666450 @default.
- W2030370574 cites W2120644189 @default.
- W2030370574 cites W2124700590 @default.
- W2030370574 cites W2131194303 @default.
- W2030370574 cites W2137052570 @default.
- W2030370574 cites W2142889871 @default.
- W2030370574 cites W2153243435 @default.
- W2030370574 cites W2158937651 @default.
- W2030370574 cites W2160272259 @default.
- W2030370574 cites W2167597644 @default.
- W2030370574 cites W2169614686 @default.
- W2030370574 cites W2169790726 @default.
- W2030370574 cites W2170027513 @default.
- W2030370574 cites W2267149019 @default.
- W2030370574 cites W4293247451 @default.
- W2030370574 doi "https://doi.org/10.1002/jcb.21632" @default.
- W2030370574 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/18044716" @default.
- W2030370574 hasPublicationYear "2008" @default.
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