FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2030378408> ?p ?o ?g. }

• W2030378408 endingPage "2154" @default.
• W2030378408 startingPage "2148" @default.
• W2030378408 abstract "BackgroundIntravascular hemolysis occurs after blood transfusion, in hemolytic anemias, and in other conditions, and is associated with hypercoagulable states. Hemolysis has been shown to potently activate platelets in vitro and in vivo, and several mechanisms have been suggested to account for this, including: (i) direct activation by hemoglobin (Hb); (ii) increase in reactive oxygen species (ROS); (iii) scavenging of nitric oxide (NO) by released Hb; and (iv) release of intraerythrocytic ADP.ObjectiveTo elucidate the mechanism of hemolysis‐mediated platelet activation.MethodsWe used flow cytometry to detect PAC‐1 binding to activated platelets for in vitro experiments, and a Siemens' Advia 120 hematology system to assess platelet aggregation by using platelet counts from in vivo experiments in a rodent model.ResultsWe found that Hb did not directly activate platelets. However, ADP bound to Hb could cause platelet activation. Furthermore, platelet activation caused by shearing of red blood cells (RBCs) was reduced in the presence of apyrase, which metabolizes ADP to AMP. The use of ROS scavengers did not affect platelet activation. We also found that cell‐free Hb enhanced platelet activation by abrogating the inhibitory effect of NO on platelet activation. In vivo infusions of ADP and purified (ADP‐free) Hb, as well as hemolysate, resulted in platelet aggregation, as shown by decreased platelet counts.ConclusionTwo primary mechanisms account for RBC hemolysis‐associated platelet activation: ADP release, which activates platelets; and cell‐free Hb release, which enhances platelet activation by lowering NO bioavailability." @default.
• W2030378408 created "2016-06-24" @default.
• W2030378408 creator A5002768213 @default.
• W2030378408 creator A5004609511 @default.
• W2030378408 creator A5007944034 @default.
• W2030378408 creator A5019373727 @default.
• W2030378408 creator A5038627792 @default.
• W2030378408 creator A5055324435 @default.
• W2030378408 creator A5066832003 @default.
• W2030378408 creator A5079729144 @default.
• W2030378408 creator A5083512645 @default.
• W2030378408 creator A5088989949 @default.
• W2030378408 creator A5089663328 @default.
• W2030378408 date "2013-12-01" @default.
• W2030378408 modified "2023-10-18" @default.
• W2030378408 title "Mechanisms of hemolysis‐associated platelet activation" @default.
• W2030378408 cites W11598776 @default.
• W2030378408 cites W1581909905 @default.
• W2030378408 cites W1967511337 @default.
• W2030378408 cites W1970796889 @default.
• W2030378408 cites W1973568641 @default.
• W2030378408 cites W1977136153 @default.
• W2030378408 cites W1982861332 @default.
• W2030378408 cites W1984201876 @default.
• W2030378408 cites W1986953737 @default.
• W2030378408 cites W1990851467 @default.
• W2030378408 cites W1990941291 @default.
• W2030378408 cites W1990999296 @default.
• W2030378408 cites W1992712202 @default.
• W2030378408 cites W1993924886 @default.
• W2030378408 cites W1996924061 @default.
• W2030378408 cites W2001078662 @default.
• W2030378408 cites W2002074235 @default.
• W2030378408 cites W2011047569 @default.
• W2030378408 cites W2014829368 @default.
• W2030378408 cites W2016116473 @default.
• W2030378408 cites W2024621079 @default.
• W2030378408 cites W2027259889 @default.
• W2030378408 cites W2034906784 @default.
• W2030378408 cites W2035616656 @default.
• W2030378408 cites W2038552179 @default.
• W2030378408 cites W2045980084 @default.
• W2030378408 cites W2049292864 @default.
• W2030378408 cites W2052080133 @default.
• W2030378408 cites W2052608119 @default.
• W2030378408 cites W2054224182 @default.
• W2030378408 cites W2058188211 @default.
• W2030378408 cites W2063782257 @default.
• W2030378408 cites W2064934027 @default.
• W2030378408 cites W2066289360 @default.
• W2030378408 cites W2093841568 @default.
• W2030378408 cites W2108576049 @default.
• W2030378408 cites W2113684546 @default.
• W2030378408 cites W2131155793 @default.
• W2030378408 cites W2150027863 @default.
• W2030378408 cites W2167589629 @default.
• W2030378408 cites W2170062988 @default.
• W2030378408 cites W2170995347 @default.
• W2030378408 cites W2174834499 @default.
• W2030378408 cites W2405449083 @default.
• W2030378408 cites W2405738162 @default.
• W2030378408 cites W4241694836 @default.
• W2030378408 cites W4290404170 @default.
• W2030378408 cites W54306856 @default.
• W2030378408 doi "https://doi.org/10.1111/jth.12422" @default.
• W2030378408 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3947421" @default.
• W2030378408 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24119131" @default.
• W2030378408 hasPublicationYear "2013" @default.
• W2030378408 type Work @default.
• W2030378408 sameAs 2030378408 @default.
• W2030378408 citedByCount "136" @default.
• W2030378408 countsByYear W20303784082014 @default.
• W2030378408 countsByYear W20303784082015 @default.
• W2030378408 countsByYear W20303784082016 @default.
• W2030378408 countsByYear W20303784082017 @default.
• W2030378408 countsByYear W20303784082018 @default.
• W2030378408 countsByYear W20303784082019 @default.
• W2030378408 countsByYear W20303784082020 @default.
• W2030378408 countsByYear W20303784082021 @default.
• W2030378408 countsByYear W20303784082022 @default.
• W2030378408 countsByYear W20303784082023 @default.
• W2030378408 crossrefType "journal-article" @default.
• W2030378408 hasAuthorship W2030378408A5002768213 @default.
• W2030378408 hasAuthorship W2030378408A5004609511 @default.
• W2030378408 hasAuthorship W2030378408A5007944034 @default.
• W2030378408 hasAuthorship W2030378408A5019373727 @default.
• W2030378408 hasAuthorship W2030378408A5038627792 @default.
• W2030378408 hasAuthorship W2030378408A5055324435 @default.
• W2030378408 hasAuthorship W2030378408A5066832003 @default.
• W2030378408 hasAuthorship W2030378408A5079729144 @default.
• W2030378408 hasAuthorship W2030378408A5083512645 @default.
• W2030378408 hasAuthorship W2030378408A5088989949 @default.
• W2030378408 hasAuthorship W2030378408A5089663328 @default.
• W2030378408 hasBestOaLocation W20303784081 @default.
• W2030378408 hasConcept C150903083 @default.
• W2030378408 hasConcept C178790620 @default.
• W2030378408 hasConcept C185592680 @default.
• W2030378408 hasConcept C202751555 @default.

• next