Matches in SemOpenAlex for { <https://semopenalex.org/work/W2030385445> ?p ?o ?g. }
Showing items 1 to 92 of
92
with 100 items per page.
- W2030385445 endingPage "320" @default.
- W2030385445 startingPage "314" @default.
- W2030385445 abstract "Superoxide dismutases (SODs) catalyze the dismutation of superoxide radicals in a broad range of organisms, including plants. Quantification of SOD activity in crude plant extracts has been problematic due to the presence of compounds that interfere with the dose-response of the assay. Although strategies exist to partially purify SODs from plant extracts, the requirement for purification limits the rapidity and practical number of assays that can be conducted. In this article, we describe modification of a procedure using o-dianisidine as substrate that permits relatively rapid quantification of SOD activity in crude leaf extracts in a microplate format. The method employs the use of a commercial apparatus that permits lysis of 12 tissue samples at once and the use of Pipes buffer to reduce interference from compounds present in crude leaf extracts. The assay provided a linear response from 1 to 50 units of SOD. The utility of the assay was demonstrated using tissue extracts prepared from a group of taxonomically diverse plants. Reaction rates with tissue extracts from two grasses were linear for at least 60 min. Tissues of certain species contained interfering compounds, most of which could be removed by ultrafiltration. The presence of plant catalases, peroxidases, and ascorbate in physiological quantities did not interfere with the assay. This approach provides a means to quantify SOD activity in relatively large numbers of plant samples provided that the possibility for the presence of interfering compounds is considered. The presence of interfering compounds in certain plant tissues necessitates caution in interpreting the effects of plant stresses on SOD." @default.
- W2030385445 created "2016-06-24" @default.
- W2030385445 creator A5026114513 @default.
- W2030385445 creator A5040190050 @default.
- W2030385445 creator A5074093064 @default.
- W2030385445 creator A5074308925 @default.
- W2030385445 date "2004-09-01" @default.
- W2030385445 modified "2023-09-26" @default.
- W2030385445 title "Microplate quantification of plant leaf superoxide dismutases" @default.
- W2030385445 cites W1999923675 @default.
- W2030385445 cites W2001562153 @default.
- W2030385445 cites W2021714464 @default.
- W2030385445 cites W2044499660 @default.
- W2030385445 cites W2053898989 @default.
- W2030385445 cites W2066020364 @default.
- W2030385445 cites W2083622913 @default.
- W2030385445 cites W2088866343 @default.
- W2030385445 cites W2089066583 @default.
- W2030385445 cites W2090967079 @default.
- W2030385445 cites W2098306477 @default.
- W2030385445 cites W2100720103 @default.
- W2030385445 cites W2100837269 @default.
- W2030385445 cites W2101422765 @default.
- W2030385445 cites W2112840757 @default.
- W2030385445 cites W4231524173 @default.
- W2030385445 cites W829757908 @default.
- W2030385445 doi "https://doi.org/10.1016/j.ab.2004.06.015" @default.
- W2030385445 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15325300" @default.
- W2030385445 hasPublicationYear "2004" @default.
- W2030385445 type Work @default.
- W2030385445 sameAs 2030385445 @default.
- W2030385445 citedByCount "42" @default.
- W2030385445 countsByYear W20303854452012 @default.
- W2030385445 countsByYear W20303854452013 @default.
- W2030385445 countsByYear W20303854452014 @default.
- W2030385445 countsByYear W20303854452015 @default.
- W2030385445 countsByYear W20303854452016 @default.
- W2030385445 countsByYear W20303854452017 @default.
- W2030385445 countsByYear W20303854452018 @default.
- W2030385445 countsByYear W20303854452019 @default.
- W2030385445 countsByYear W20303854452020 @default.
- W2030385445 countsByYear W20303854452022 @default.
- W2030385445 countsByYear W20303854452023 @default.
- W2030385445 crossrefType "journal-article" @default.
- W2030385445 hasAuthorship W2030385445A5026114513 @default.
- W2030385445 hasAuthorship W2030385445A5040190050 @default.
- W2030385445 hasAuthorship W2030385445A5074093064 @default.
- W2030385445 hasAuthorship W2030385445A5074308925 @default.
- W2030385445 hasConcept C162008176 @default.
- W2030385445 hasConcept C181199279 @default.
- W2030385445 hasConcept C185592680 @default.
- W2030385445 hasConcept C2775838275 @default.
- W2030385445 hasConcept C2992076609 @default.
- W2030385445 hasConcept C43617362 @default.
- W2030385445 hasConcept C50817676 @default.
- W2030385445 hasConcept C55493867 @default.
- W2030385445 hasConcept C57409179 @default.
- W2030385445 hasConcept C59822182 @default.
- W2030385445 hasConcept C86803240 @default.
- W2030385445 hasConceptScore W2030385445C162008176 @default.
- W2030385445 hasConceptScore W2030385445C181199279 @default.
- W2030385445 hasConceptScore W2030385445C185592680 @default.
- W2030385445 hasConceptScore W2030385445C2775838275 @default.
- W2030385445 hasConceptScore W2030385445C2992076609 @default.
- W2030385445 hasConceptScore W2030385445C43617362 @default.
- W2030385445 hasConceptScore W2030385445C50817676 @default.
- W2030385445 hasConceptScore W2030385445C55493867 @default.
- W2030385445 hasConceptScore W2030385445C57409179 @default.
- W2030385445 hasConceptScore W2030385445C59822182 @default.
- W2030385445 hasConceptScore W2030385445C86803240 @default.
- W2030385445 hasIssue "2" @default.
- W2030385445 hasLocation W20303854451 @default.
- W2030385445 hasLocation W20303854452 @default.
- W2030385445 hasOpenAccess W2030385445 @default.
- W2030385445 hasPrimaryLocation W20303854451 @default.
- W2030385445 hasRelatedWork W1970910281 @default.
- W2030385445 hasRelatedWork W2000793212 @default.
- W2030385445 hasRelatedWork W2020371940 @default.
- W2030385445 hasRelatedWork W2069842851 @default.
- W2030385445 hasRelatedWork W2343960475 @default.
- W2030385445 hasRelatedWork W2357441241 @default.
- W2030385445 hasRelatedWork W2406847408 @default.
- W2030385445 hasRelatedWork W2592360891 @default.
- W2030385445 hasRelatedWork W2744364675 @default.
- W2030385445 hasRelatedWork W4312838830 @default.
- W2030385445 hasVolume "332" @default.
- W2030385445 isParatext "false" @default.
- W2030385445 isRetracted "false" @default.
- W2030385445 magId "2030385445" @default.
- W2030385445 workType "article" @default.