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- W2030406711 abstract "Photolytic optical gating (POG) facilitates rapid, on-line and highly sensitive analyses, though POG utilizes UV lasers for sample injection. We present a low-cost, more portable alternative, employing an ultraviolet light-emitting diode (UV-LED) array to inject caged fluorescent dyes via photolysis. Utilizing the UV-LED array, labeled amino acids were injected with nanomolar limits of detection (270 ± 30 nM and 250 ± 30 nM for arginine and citrulline, respectively). When normalized for the difference in light intensity, the UV-LED array provides comparable sensitivity to POG utilizing UV lasers. Additionally, the UV-LED array yielded sufficient beam quality and stability to facilitate coupling with a Hadamard transform, resulting in increased sensitivity. This work shows, for the first time, the use of an UV-LED for online POG with comparable sensitivity to conventional laser sources but at a lower cost." @default.
- W2030406711 created "2016-06-24" @default.
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- W2030406711 date "2012-08-22" @default.
- W2030406711 modified "2023-09-27" @default.
- W2030406711 title "Online photolytic optical gating of caged fluorophores in capillary zone electrophoresis utilizing an ultraviolet light-emitting diode" @default.
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- W2030406711 doi "https://doi.org/10.1002/elps.201200279" @default.
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