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- W2030482849 abstract "Cotransformation of Nicotiana tabacum was done using a single Agrobacterium tumefaciens strain harbouring a cointegrate vector (one copy per cell) and a binary vector (ten to 15 copies per cell). The T-DNAs of the cointegrate plasmid, pGV2260::pSSJ1 and the binary plasmid, pGA472 carried hph and nptII as plant selection markers, respectively. When the binary T-DNA marker (nptII) was used for selection, the non-selected cointegrate T-DNA with hph cotransformed at 36% frequency. However, upon using the cointegrate T-DNA with hph for selection, cotransformation of binary T-DNA with nptII was much higher (56–74%). Segregation of the T-DNA markers hph and nptII was followed in the T1 generation to screen for the elimination of the selection marker. The elimination of nptII of the multicopy binary vector was found in the progeny of only one of the three T0 plants and at a low frequency of 3%. However, elimination of hph of the single-copy cointegrate vector was found at 16–18% frequency in the progeny of both the T0 plants analysed. The use of the T-DNA of low copy number cointegrate vector for initial selection improves the cotransformation frequency of non-selected T-DNA of the multicopy binary vector. This strategy also increases the frequency of generation of selection marker-free transgenic plants in the T1 generation." @default.
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- W2030482849 date "2002-10-01" @default.
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- W2030482849 title "Generation of selection marker-free transgenic plants by cotransformation of a cointegrate vector T-DNA and a binary vector T-DNA in one Agrobacterium tumefaciens strain" @default.
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- W2030482849 doi "https://doi.org/10.1016/s0168-9452(02)00215-7" @default.
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