Matches in SemOpenAlex for { <https://semopenalex.org/work/W2030489009> ?p ?o ?g. }
Showing items 1 to 69 of
69
with 100 items per page.
- W2030489009 abstract "Soluble N-ethylmaleimide-sensitive factor attachment receptor (SNARE) proteins are evolutionarily conserved molecule machines that couple their folding/assembly to drive diverse intracellular membrane fusion. In particular, neuronal SNAREs mediate extremely fast and calcium-triggered fusion of synaptic vesicles in nerve endings for neurotransmission. It remains unclear how SNAREs fold and how such folding is coupled to membrane fusion and regulated in response to different stimuli. To address these questions, we have characterized the folding energy and kinetics of four representative SNARE complexes at a single-molecule level using high-resolution optical tweezers. Despite their dramatically different fusion rate, all four SNARE complexes assemble by the same step-wise zippering mechanism: slow N-terminal domain (NTD) association, a pause in a force-dependent half-zippered intermediate and fast C-terminal domain (CTD) zippering. However, the energy release from CTD zippering differs from 13 kBT to 28 kBT for yeast and neuron SNARE complexes, respectively. We suggest that SNARE complexes share a conserved zippering pathway to efficiently drive membrane fusion, but release different amount of energy to control the fusion speed. Finally, we will show our latest results about the roles of SNARE mutations, Munc18-1, complexin, and Vc peptides in SNARE zippering and membrane fusion." @default.
- W2030489009 created "2016-06-24" @default.
- W2030489009 creator A5005359731 @default.
- W2030489009 date "2015-01-01" @default.
- W2030489009 modified "2023-09-26" @default.
- W2030489009 title "Energetics and Kinetics of SNARE Zippering and Regulation Revealed by Single-Molecule Manipulation Approach" @default.
- W2030489009 doi "https://doi.org/10.1016/j.bpj.2014.11.1938" @default.
- W2030489009 hasPublicationYear "2015" @default.
- W2030489009 type Work @default.
- W2030489009 sameAs 2030489009 @default.
- W2030489009 citedByCount "0" @default.
- W2030489009 crossrefType "journal-article" @default.
- W2030489009 hasAuthorship W2030489009A5005359731 @default.
- W2030489009 hasBestOaLocation W20304890091 @default.
- W2030489009 hasConcept C103038307 @default.
- W2030489009 hasConcept C119599485 @default.
- W2030489009 hasConcept C12554922 @default.
- W2030489009 hasConcept C127413603 @default.
- W2030489009 hasConcept C130316041 @default.
- W2030489009 hasConcept C148785051 @default.
- W2030489009 hasConcept C185592680 @default.
- W2030489009 hasConcept C196330066 @default.
- W2030489009 hasConcept C2776545253 @default.
- W2030489009 hasConcept C2908790754 @default.
- W2030489009 hasConcept C41625074 @default.
- W2030489009 hasConcept C55493867 @default.
- W2030489009 hasConcept C86803240 @default.
- W2030489009 hasConcept C95444343 @default.
- W2030489009 hasConceptScore W2030489009C103038307 @default.
- W2030489009 hasConceptScore W2030489009C119599485 @default.
- W2030489009 hasConceptScore W2030489009C12554922 @default.
- W2030489009 hasConceptScore W2030489009C127413603 @default.
- W2030489009 hasConceptScore W2030489009C130316041 @default.
- W2030489009 hasConceptScore W2030489009C148785051 @default.
- W2030489009 hasConceptScore W2030489009C185592680 @default.
- W2030489009 hasConceptScore W2030489009C196330066 @default.
- W2030489009 hasConceptScore W2030489009C2776545253 @default.
- W2030489009 hasConceptScore W2030489009C2908790754 @default.
- W2030489009 hasConceptScore W2030489009C41625074 @default.
- W2030489009 hasConceptScore W2030489009C55493867 @default.
- W2030489009 hasConceptScore W2030489009C86803240 @default.
- W2030489009 hasConceptScore W2030489009C95444343 @default.
- W2030489009 hasLocation W20304890091 @default.
- W2030489009 hasOpenAccess W2030489009 @default.
- W2030489009 hasPrimaryLocation W20304890091 @default.
- W2030489009 hasRelatedWork W113080609 @default.
- W2030489009 hasRelatedWork W1732663100 @default.
- W2030489009 hasRelatedWork W1980906940 @default.
- W2030489009 hasRelatedWork W1990558068 @default.
- W2030489009 hasRelatedWork W1999702498 @default.
- W2030489009 hasRelatedWork W2104374433 @default.
- W2030489009 hasRelatedWork W2104536170 @default.
- W2030489009 hasRelatedWork W2121766064 @default.
- W2030489009 hasRelatedWork W2313890271 @default.
- W2030489009 hasRelatedWork W2462291260 @default.
- W2030489009 hasRelatedWork W2557708678 @default.
- W2030489009 hasRelatedWork W2574505563 @default.
- W2030489009 hasRelatedWork W2765551049 @default.
- W2030489009 hasRelatedWork W2802354374 @default.
- W2030489009 hasRelatedWork W2888333487 @default.
- W2030489009 hasRelatedWork W2937132049 @default.
- W2030489009 hasRelatedWork W2951496883 @default.
- W2030489009 hasRelatedWork W3022379701 @default.
- W2030489009 hasRelatedWork W3119914811 @default.
- W2030489009 hasRelatedWork W569096600 @default.
- W2030489009 isParatext "false" @default.
- W2030489009 isRetracted "false" @default.
- W2030489009 magId "2030489009" @default.
- W2030489009 workType "article" @default.