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- W2030492386 abstract "A strain of Aspergillus niger, previously isolated from sugarcane bagasse because of its capacity to degrade phenanthrene in soil by solid culture, was used to express a manganese peroxidase gene (mnp1) from Phanerochaete chrysosporium, aiming at increasing its polycyclic aromatic hydrocarbons degradation capacity. Transformants were selected based on their resistance to hygromycin B and the discoloration induced on Poly R-478 dye by the peroxidase activity. The recombinant A. niger SBC2-T3 strain developed MnP activity and was able to remove 95% of the initial phenanthrene (400 ppm) from a microcosm soil system after 17 days, whereas the wild strain removed 72% under the same conditions. Transformation success was confirmed by PCR amplification using gene-specific primers, and a single fragment (1,348 bp long, as expected) of the recombinant mnp1 was amplified in the DNA from transformants, which was absent from the parental strain." @default.
- W2030492386 created "2016-06-24" @default.
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- W2030492386 date "2011-01-01" @default.
- W2030492386 modified "2023-10-18" @default.
- W2030492386 title "Heterologous Expression of Manganese Peroxidase in Aspergillus niger and Its Effect on Phenanthrene Removal from Soil" @default.
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- W2030492386 doi "https://doi.org/10.1159/000331563" @default.
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