Matches in SemOpenAlex for { <https://semopenalex.org/work/W2030497785> ?p ?o ?g. }
Showing items 1 to 78 of
78
with 100 items per page.
- W2030497785 endingPage "14266" @default.
- W2030497785 startingPage "14257" @default.
- W2030497785 abstract "The impact of p13suc1 on the conformation and regulation of cyclin-dependent kinases (cdks) and cyclins was investigated by spectroscopic and rapid kinetic approaches. In the absence of phosphorylation on cdks, p13suc1 formed stable complexes, mainly stabilized by hydrophobic interactions, specifically with cdk2 and cdc2. The presence of cyclin A, associated with cdk2 or cdc2, increased the stability of the interaction between cdk2 and p13suc1 by a factor of 2. However, cyclin A did not modify the association rate of p13suc1 to cdk2, but the dissociation rate, which was decreased 3-fold. Moreover, binding of p13suc1 to cdk2 resulted in a 2-fold decrease in the release of nucleotide from cdk2, indicating that p13suc1 induces a marked change in the structure of the nucleotide binding site of cdks. On the basis of the structure of cdk2/CksHs1 complex and on our kinetic results, we propose that the binding of Cks proteins to C-lobe of cdk2 is stabilized by the presence of cyclin A and that it may modify the orientation of the loop carrying residues 14 and 15 and their consequent access for dephosphorylation by cdc25 phosphatases. Finally, we have shown that dimerization of p13suc1 in the presence of zinc abolishes its interaction with cdks, which suggests that the binding of p13suc1 to cdk2 or cdk2/cyclin A may be regulated by dimerization of p13suc1 in vivo." @default.
- W2030497785 created "2016-06-24" @default.
- W2030497785 creator A5018056053 @default.
- W2030497785 creator A5051380897 @default.
- W2030497785 creator A5057982935 @default.
- W2030497785 date "1998-09-16" @default.
- W2030497785 modified "2023-10-03" @default.
- W2030497785 title "Kinetics of Dimerization and Interactions of p13<i><sup>suc1</sup></i> with Cyclin-Dependent Kinases" @default.
- W2030497785 cites W2070586397 @default.
- W2030497785 doi "https://doi.org/10.1021/bi980913u" @default.
- W2030497785 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9760264" @default.
- W2030497785 hasPublicationYear "1998" @default.
- W2030497785 type Work @default.
- W2030497785 sameAs 2030497785 @default.
- W2030497785 citedByCount "12" @default.
- W2030497785 countsByYear W20304977852012 @default.
- W2030497785 countsByYear W20304977852022 @default.
- W2030497785 crossrefType "journal-article" @default.
- W2030497785 hasAuthorship W2030497785A5018056053 @default.
- W2030497785 hasAuthorship W2030497785A5051380897 @default.
- W2030497785 hasAuthorship W2030497785A5057982935 @default.
- W2030497785 hasConcept C120089663 @default.
- W2030497785 hasConcept C120504264 @default.
- W2030497785 hasConcept C124320809 @default.
- W2030497785 hasConcept C12554922 @default.
- W2030497785 hasConcept C1491633281 @default.
- W2030497785 hasConcept C16438837 @default.
- W2030497785 hasConcept C175738201 @default.
- W2030497785 hasConcept C184235292 @default.
- W2030497785 hasConcept C185592680 @default.
- W2030497785 hasConcept C2778583388 @default.
- W2030497785 hasConcept C28179978 @default.
- W2030497785 hasConcept C29537977 @default.
- W2030497785 hasConcept C55493867 @default.
- W2030497785 hasConcept C82495950 @default.
- W2030497785 hasConcept C86803240 @default.
- W2030497785 hasConcept C94561458 @default.
- W2030497785 hasConcept C97029542 @default.
- W2030497785 hasConceptScore W2030497785C120089663 @default.
- W2030497785 hasConceptScore W2030497785C120504264 @default.
- W2030497785 hasConceptScore W2030497785C124320809 @default.
- W2030497785 hasConceptScore W2030497785C12554922 @default.
- W2030497785 hasConceptScore W2030497785C1491633281 @default.
- W2030497785 hasConceptScore W2030497785C16438837 @default.
- W2030497785 hasConceptScore W2030497785C175738201 @default.
- W2030497785 hasConceptScore W2030497785C184235292 @default.
- W2030497785 hasConceptScore W2030497785C185592680 @default.
- W2030497785 hasConceptScore W2030497785C2778583388 @default.
- W2030497785 hasConceptScore W2030497785C28179978 @default.
- W2030497785 hasConceptScore W2030497785C29537977 @default.
- W2030497785 hasConceptScore W2030497785C55493867 @default.
- W2030497785 hasConceptScore W2030497785C82495950 @default.
- W2030497785 hasConceptScore W2030497785C86803240 @default.
- W2030497785 hasConceptScore W2030497785C94561458 @default.
- W2030497785 hasConceptScore W2030497785C97029542 @default.
- W2030497785 hasIssue "40" @default.
- W2030497785 hasLocation W20304977851 @default.
- W2030497785 hasLocation W20304977852 @default.
- W2030497785 hasLocation W20304977853 @default.
- W2030497785 hasOpenAccess W2030497785 @default.
- W2030497785 hasPrimaryLocation W20304977851 @default.
- W2030497785 hasRelatedWork W1834547194 @default.
- W2030497785 hasRelatedWork W1983018722 @default.
- W2030497785 hasRelatedWork W2016189963 @default.
- W2030497785 hasRelatedWork W2030497785 @default.
- W2030497785 hasRelatedWork W2057290799 @default.
- W2030497785 hasRelatedWork W2092104789 @default.
- W2030497785 hasRelatedWork W2125555976 @default.
- W2030497785 hasRelatedWork W2162150896 @default.
- W2030497785 hasRelatedWork W4243458619 @default.
- W2030497785 hasRelatedWork W4250109928 @default.
- W2030497785 hasVolume "37" @default.
- W2030497785 isParatext "false" @default.
- W2030497785 isRetracted "false" @default.
- W2030497785 magId "2030497785" @default.
- W2030497785 workType "article" @default.