Matches in SemOpenAlex for { <https://semopenalex.org/work/W2030505259> ?p ?o ?g. }
Showing items 1 to 89 of
89
with 100 items per page.
- W2030505259 endingPage "627" @default.
- W2030505259 startingPage "623" @default.
- W2030505259 abstract "Successful DNA typing after rape is limited when only a few sperm and numerous vaginal cells are recovered from a swab, resulting in an extremely unfavorable ratio of male to female DNA. The goal of this study was to develop a protocol involving sperm cell sorting with flow cytometry based on differences in ploidy, major histocompatibility class I, CD45, and cytokeratin expression.Vaginal lavages were mixed with serially diluted ejaculate. After immunostaining and stoichiometric nuclear staining, spermatocytes were isolated by fluorescence-activated cell sorting. All sorted cells were used for DNA extraction and subsequent quantitative fluorescent multiplex polymerase chain reaction. The preferential lysis was performed for comparison.The sorting procedure was superior to the preferential lysis method within all tested conditions. In unfavorable dilutions, the male DNA could be identified only after cell sorting with flow cytometry.We were able to show that separation of sperm and vaginal cells using cell sorting with flow cytometry may be crucial when there are only microtraces of sperm detectable after rape." @default.
- W2030505259 created "2016-06-24" @default.
- W2030505259 creator A5019174448 @default.
- W2030505259 creator A5040766812 @default.
- W2030505259 creator A5058526383 @default.
- W2030505259 creator A5080805278 @default.
- W2030505259 date "1999-10-01" @default.
- W2030505259 modified "2023-10-11" @default.
- W2030505259 title "Separation of sperm and vaginal cells with flow cytometry for DNA typing after sexual assault" @default.
- W2030505259 cites W125751719 @default.
- W2030505259 cites W1966420972 @default.
- W2030505259 cites W1977379434 @default.
- W2030505259 cites W1986090838 @default.
- W2030505259 cites W1998165384 @default.
- W2030505259 cites W2004708117 @default.
- W2030505259 cites W2013214117 @default.
- W2030505259 cites W2021583348 @default.
- W2030505259 cites W2033554233 @default.
- W2030505259 cites W2088096907 @default.
- W2030505259 cites W2144634347 @default.
- W2030505259 cites W2148862179 @default.
- W2030505259 cites W2409962686 @default.
- W2030505259 doi "https://doi.org/10.1016/s0029-7844(99)00373-7" @default.
- W2030505259 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10511370" @default.
- W2030505259 hasPublicationYear "1999" @default.
- W2030505259 type Work @default.
- W2030505259 sameAs 2030505259 @default.
- W2030505259 citedByCount "35" @default.
- W2030505259 countsByYear W20305052592012 @default.
- W2030505259 countsByYear W20305052592013 @default.
- W2030505259 countsByYear W20305052592014 @default.
- W2030505259 countsByYear W20305052592015 @default.
- W2030505259 countsByYear W20305052592016 @default.
- W2030505259 countsByYear W20305052592017 @default.
- W2030505259 countsByYear W20305052592018 @default.
- W2030505259 countsByYear W20305052592021 @default.
- W2030505259 countsByYear W20305052592022 @default.
- W2030505259 countsByYear W20305052592023 @default.
- W2030505259 crossrefType "journal-article" @default.
- W2030505259 hasAuthorship W2030505259A5019174448 @default.
- W2030505259 hasAuthorship W2030505259A5040766812 @default.
- W2030505259 hasAuthorship W2030505259A5058526383 @default.
- W2030505259 hasAuthorship W2030505259A5080805278 @default.
- W2030505259 hasConcept C104317684 @default.
- W2030505259 hasConcept C153911025 @default.
- W2030505259 hasConcept C16685009 @default.
- W2030505259 hasConcept C2778093475 @default.
- W2030505259 hasConcept C2778630268 @default.
- W2030505259 hasConcept C2781087480 @default.
- W2030505259 hasConcept C49105822 @default.
- W2030505259 hasConcept C54355233 @default.
- W2030505259 hasConcept C553184892 @default.
- W2030505259 hasConcept C71924100 @default.
- W2030505259 hasConcept C74864618 @default.
- W2030505259 hasConcept C86803240 @default.
- W2030505259 hasConceptScore W2030505259C104317684 @default.
- W2030505259 hasConceptScore W2030505259C153911025 @default.
- W2030505259 hasConceptScore W2030505259C16685009 @default.
- W2030505259 hasConceptScore W2030505259C2778093475 @default.
- W2030505259 hasConceptScore W2030505259C2778630268 @default.
- W2030505259 hasConceptScore W2030505259C2781087480 @default.
- W2030505259 hasConceptScore W2030505259C49105822 @default.
- W2030505259 hasConceptScore W2030505259C54355233 @default.
- W2030505259 hasConceptScore W2030505259C553184892 @default.
- W2030505259 hasConceptScore W2030505259C71924100 @default.
- W2030505259 hasConceptScore W2030505259C74864618 @default.
- W2030505259 hasConceptScore W2030505259C86803240 @default.
- W2030505259 hasIssue "4" @default.
- W2030505259 hasLocation W20305052591 @default.
- W2030505259 hasLocation W20305052592 @default.
- W2030505259 hasOpenAccess W2030505259 @default.
- W2030505259 hasPrimaryLocation W20305052591 @default.
- W2030505259 hasRelatedWork W1667924102 @default.
- W2030505259 hasRelatedWork W1836470942 @default.
- W2030505259 hasRelatedWork W2056551061 @default.
- W2030505259 hasRelatedWork W2133709357 @default.
- W2030505259 hasRelatedWork W2273584345 @default.
- W2030505259 hasRelatedWork W2370897094 @default.
- W2030505259 hasRelatedWork W2381800003 @default.
- W2030505259 hasRelatedWork W2408616169 @default.
- W2030505259 hasRelatedWork W4296474603 @default.
- W2030505259 hasRelatedWork W99322424 @default.
- W2030505259 hasVolume "94" @default.
- W2030505259 isParatext "false" @default.
- W2030505259 isRetracted "false" @default.
- W2030505259 magId "2030505259" @default.
- W2030505259 workType "article" @default.