FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2030539548> ?p ?o ?g. }

• W2030539548 abstract "Proceedings: AACR 103rd Annual Meeting 2012‐‐ Mar 31‐Apr 4, 2012; Chicago, ILPurpose: Many cancers initially respond to Cisplatin-based chemotherapy; however, resistance frequently develops. We have recently reported that reduction of IGFBP-3 expression by promoter methylation is involved in the CDDP acquired resistance process in 3 matched CDDP sensitive/resistant human cancer cell lines and in a human cohort of 36 in non-small cell lung cancer (NSCLC) patients, probably because CDDP also induces DNA methylation de novo. The purpose of the present study is to investigate the role of IGFBP-3 in the PI3K signaling pathway alterations to design a translational based-profile to predict resistance in NSCLC. The biological significance of IGFBP-3 is of great importance in controlling cell growth, transformation and survival; as IGF-I binds to IGFBP-3 with stronger affinity than to its own receptor (IGFIR), blocking their interaction and abolishing the mitogenic and antiapoptotic actions. IGF-I is also able to activate EGF receptor. Those tyrosine kinases receptors (IGFIR and EGFR), signal through the PI3K/Akt pathway, that plays a crucial role in cell growth, proliferation, and survival and is commonly upregulated during tumorigenesis, including NSCLC; although the precise mechanism is not well defined. Patients and methods: In the present study we have examined the relationship between first, IGFBP-3 gene expression regulated by promoter methylation measured by RT-PCR, bisulfite sequencing and methylation specific PCR and second the activation of the EGFR, IGFIR and PI3K/AKT pathways through the analysis of the PTEN, AKT, pAKT, pEGFR, EGFR, pIGFIR and IGFIR protein levels by Western-Blot, immunofluorescence and immunohistochemical analysis in 10 human cancer cell lines and in 25 NSCLC patients with known IGFBP-3 methylation status and response to CDDP. Additionally, in order to provide a helpful tool that enables clinicians to identify or to exclude patients with a potential response to cisplatin, we have used the Fisher's exact test within a 2x2 contingence table to calculate the association between our diagnostic test and the true outcome of analyzed samples in terms of cisplatin IC50. Results: Our results suggest that loss of IGFBP-3 expression by promoter methylation in tumor cells treated with CDDP may activate the PI3K/AKT pathway through the specific derepression of IGFIR signaling, inducing resistance to CDDP. This study also provides a predictive test for clinical practice with an accuracy and precision of 0.84 and 0.9, respectively, (P=0.0062). Conclusion: We present a biomarker-test that could provide clinicians with a robust tool with which to decide on the use of cisplatin, improving patient clinical outcomes. Supported by FIS project number: PS09/00472 and a Miguel Servet financial grant to Ibanez de Caceres, I (CP08/000689; PI-717).Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 103rd Annual Meeting of the American Association for Cancer Research; 2012 Mar 31-Apr 4; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2012;72(8 Suppl):Abstract nr 1726. doi:1538-7445.AM2012-1726" @default.
• W2030539548 created "2016-06-24" @default.
• W2030539548 creator A5026127785 @default.
• W2030539548 creator A5028089976 @default.
• W2030539548 creator A5044837065 @default.
• W2030539548 creator A5049613402 @default.
• W2030539548 creator A5060547142 @default.
• W2030539548 creator A5070235024 @default.
• W2030539548 creator A5079647211 @default.
• W2030539548 creator A5083718672 @default.
• W2030539548 creator A5086551845 @default.
• W2030539548 date "2012-04-15" @default.
• W2030539548 modified "2023-10-18" @default.
• W2030539548 title "Abstract 1726: A novel biomarker panel identifies the response to CDDP treatment in NSCLC patients" @default.
• W2030539548 doi "https://doi.org/10.1158/1538-7445.am2012-1726" @default.
• W2030539548 hasPublicationYear "2012" @default.
• W2030539548 type Work @default.
• W2030539548 sameAs 2030539548 @default.
• W2030539548 citedByCount "0" @default.
• W2030539548 crossrefType "proceedings-article" @default.
• W2030539548 hasAuthorship W2030539548A5026127785 @default.
• W2030539548 hasAuthorship W2030539548A5028089976 @default.
• W2030539548 hasAuthorship W2030539548A5044837065 @default.
• W2030539548 hasAuthorship W2030539548A5049613402 @default.
• W2030539548 hasAuthorship W2030539548A5060547142 @default.
• W2030539548 hasAuthorship W2030539548A5070235024 @default.
• W2030539548 hasAuthorship W2030539548A5079647211 @default.
• W2030539548 hasAuthorship W2030539548A5083718672 @default.
• W2030539548 hasAuthorship W2030539548A5086551845 @default.
• W2030539548 hasConcept C101544691 @default.
• W2030539548 hasConcept C104317684 @default.
• W2030539548 hasConcept C121608353 @default.
• W2030539548 hasConcept C127561419 @default.
• W2030539548 hasConcept C150194340 @default.
• W2030539548 hasConcept C189235521 @default.
• W2030539548 hasConcept C190727270 @default.
• W2030539548 hasConcept C2776694085 @default.
• W2030539548 hasConcept C2777609662 @default.
• W2030539548 hasConcept C2778239845 @default.
• W2030539548 hasConcept C33288867 @default.
• W2030539548 hasConcept C502942594 @default.
• W2030539548 hasConcept C54355233 @default.
• W2030539548 hasConcept C555283112 @default.
• W2030539548 hasConcept C62112901 @default.
• W2030539548 hasConcept C62478195 @default.
• W2030539548 hasConcept C71924100 @default.
• W2030539548 hasConcept C75217442 @default.
• W2030539548 hasConcept C86554907 @default.
• W2030539548 hasConcept C86803240 @default.
• W2030539548 hasConcept C95444343 @default.
• W2030539548 hasConceptScore W2030539548C101544691 @default.
• W2030539548 hasConceptScore W2030539548C104317684 @default.
• W2030539548 hasConceptScore W2030539548C121608353 @default.
• W2030539548 hasConceptScore W2030539548C127561419 @default.
• W2030539548 hasConceptScore W2030539548C150194340 @default.
• W2030539548 hasConceptScore W2030539548C189235521 @default.
• W2030539548 hasConceptScore W2030539548C190727270 @default.
• W2030539548 hasConceptScore W2030539548C2776694085 @default.
• W2030539548 hasConceptScore W2030539548C2777609662 @default.
• W2030539548 hasConceptScore W2030539548C2778239845 @default.
• W2030539548 hasConceptScore W2030539548C33288867 @default.
• W2030539548 hasConceptScore W2030539548C502942594 @default.
• W2030539548 hasConceptScore W2030539548C54355233 @default.
• W2030539548 hasConceptScore W2030539548C555283112 @default.
• W2030539548 hasConceptScore W2030539548C62112901 @default.
• W2030539548 hasConceptScore W2030539548C62478195 @default.
• W2030539548 hasConceptScore W2030539548C71924100 @default.
• W2030539548 hasConceptScore W2030539548C75217442 @default.
• W2030539548 hasConceptScore W2030539548C86554907 @default.
• W2030539548 hasConceptScore W2030539548C86803240 @default.
• W2030539548 hasConceptScore W2030539548C95444343 @default.
• W2030539548 hasLocation W20305395481 @default.
• W2030539548 hasOpenAccess W2030539548 @default.
• W2030539548 hasPrimaryLocation W20305395481 @default.
• W2030539548 hasRelatedWork W1488831821 @default.
• W2030539548 hasRelatedWork W1965697171 @default.
• W2030539548 hasRelatedWork W1988979114 @default.
• W2030539548 hasRelatedWork W1998550865 @default.
• W2030539548 hasRelatedWork W2007724108 @default.
• W2030539548 hasRelatedWork W2014806585 @default.
• W2030539548 hasRelatedWork W2017288701 @default.
• W2030539548 hasRelatedWork W2062472379 @default.
• W2030539548 hasRelatedWork W2072170172 @default.
• W2030539548 hasRelatedWork W2074828193 @default.
• W2030539548 hasRelatedWork W2101563790 @default.
• W2030539548 hasRelatedWork W2202197310 @default.
• W2030539548 hasRelatedWork W2328619089 @default.
• W2030539548 hasRelatedWork W2483760780 @default.
• W2030539548 hasRelatedWork W2487905146 @default.
• W2030539548 hasRelatedWork W2560504653 @default.
• W2030539548 hasRelatedWork W2562078269 @default.
• W2030539548 hasRelatedWork W2603142232 @default.
• W2030539548 hasRelatedWork W2955272593 @default.
• W2030539548 hasRelatedWork W3134511527 @default.
• W2030539548 isParatext "false" @default.
• W2030539548 isRetracted "false" @default.
• W2030539548 magId "2030539548" @default.
• W2030539548 workType "article" @default.