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- W2030572043 abstract "The Human Red Blood Cell (hRBC) is a highly specialized cell in oxygen transport and has an intracellular pH of 7.2, a Vm of -8 mV, and an average life span of 120 days. A rise of [Ca2+]i has been associated with the senescence process of the hRBC and it has been related with the mechanical stress at the microcirculation level. We have presented evidence for the existence of a mechano-activated K+ channel (HEMKCA) and a voltage-dependent K+/Ca2+-Exchanger (1)(2). The activation process of the K+/Ca2+-Exchanger has sigmoid voltage dependence and a permeability sequence of: K+>Rb+>>Cs+ and Ca2+>Ba2+>>Mg2+. We have proposed a new hypothesis for the senescence of hRBC (The hypothesis of K+). Here, when the hRBC passes throughout the capillary suffers mechanical stress, increasing its K+ permeability, depolarizing its membrane, and consequently activating the K+/Ca2+-Exchanger, letting the Ca2+ enters the hRBC. Using the Patch-Clamp technique, we measured K+/Ca2+-Exchanger currents and the effect of pH change (from 7.2 to 5.9) in the cytoplasmic side. At an acidic internal pH we registered a complex effect, i/e. the current increased when the exchanger was in the Ca2+ entry mode, while in the reverse mode a current decrease was observed. On the other hand (i/e. at pH 6.7), the temporal development of the inactivation was slowed and saturated, from about 430ms to 490ms (14%) at 160mV. These results suggest the existence of at least one histidine residue in the exchanger, exposed to the cell interior which affects its activity, and also, the existence of two different binding sites for intracellular K+ and Ca2+. We present new evidences for the existence of a K+/Ca2+-Exchanger in the hRBC and a complex effect of changes of internal pH.(1)(2005) Biophys.J.88(1):593.(2)(2006) Biophys.J.89(1):456." @default.
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- W2030572043 date "2014-01-01" @default.
- W2030572043 modified "2023-09-28" @default.
- W2030572043 title "The Human Red Blood Cell K+/Ca2+ Exchanger: Effect of Internal pH" @default.
- W2030572043 doi "https://doi.org/10.1016/j.bpj.2013.11.3221" @default.
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