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• W2030600774 abstract "Because male ovine embryos develop faster than female embryos, the transcription of SRY and ZFY, two genes located on the Y chromosome, was examined in preimplantation stages using the reverse transcriptase polymerase chain reaction (RT-PCR). RNA was extracted from pools of ovine embryos matured and fertilized in vitro then cultured in synthetic oviduct fluid medium and recovered from 24 to 207 hr post-insemination (two-cell up to hatched blastocyst stage). Since primers used to amplify ZFY also amplify the homologue ZFX, located on the X chromosome, transcripts were differentiated by digestion with restriction enzymes. ZFY and ZFX transcripts were present in all stages examined following RT-PCR, whereas transcripts for SRY were undetectable in all investigated stages following either RT nested PCR or Southern analysis. The presence of ZFY transcripts suggests that Y chromosome is transcriptionally active during early ovine preimplantation development. The possible relationship between a faster growth of male embryos and the transcription of Y-linked genes at early stages of development is discussed. © 1996 Wiley-Liss, Inc." @default.
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• W2030600774 date "1996-10-01" @default.
• W2030600774 modified "2023-09-24" @default.
• W2030600774 title "Transcription of Y- and X-linked genes in preimplantation ovine embryos" @default.
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• W2030600774 doi "https://doi.org/10.1002/(sici)1098-2795(199610)45:2<132::aid-mrd4>3.0.co;2-u" @default.
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