Matches in SemOpenAlex for { <https://semopenalex.org/work/W2030607403> ?p ?o ?g. }
Showing items 1 to 94 of
94
with 100 items per page.
- W2030607403 endingPage "394" @default.
- W2030607403 startingPage "385" @default.
- W2030607403 abstract "Affinity labelling of the estradiol-17 beta dehydrogenase of human placenta has been performed using derivatives of estradiol-17 beta carrying alkylating groups in nine different positions on the steroid nucleus. The active-site-directed character of the inhibition is confirmed by the following observations: the affinity labels are substrates or competitive inhibitors, the enzyme is protected against inactivation and alkylation by the substrate and by the coenzyme, the stoichiometry of the alkylation is two moles of inhibitor per 68 000 g of enzyme (dimer). The alkylation of a histidine residue which is fast and extensive when the alkylation side chain is on the C-3 carbon atom, is dramatically decreased when alkylating side chain is shifted towards rings B and D. These results allow the location of this histidine in the vicinity of ring A and probably on the beta face of the steroid nucleus. The reactivity of a cysteine located on the active site was quite different, showing increasing alkylation when the alkylating substituent of the affinity labels was shifted from C-3 to C-16 of the steroid nucleus. The correlation of this result and that obtained using an alkylating analog of NAD (3-chloroacetyl-pyridine-adenine dinucleotide) indicates that this cysteine is located in the catalytic region of the active site, at the junction of the ring D of the steroid nucleus with the nicotinamide moiety of the coenzyme." @default.
- W2030607403 created "2016-06-24" @default.
- W2030607403 creator A5002034237 @default.
- W2030607403 creator A5006078287 @default.
- W2030607403 creator A5024944429 @default.
- W2030607403 creator A5028108508 @default.
- W2030607403 creator A5071364492 @default.
- W2030607403 date "1976-09-01" @default.
- W2030607403 modified "2023-09-25" @default.
- W2030607403 title "Affinity Labelling of the Estradiol-17beta Dehydrogenase from Human Placenta with Substrate Analogs" @default.
- W2030607403 cites W1518598430 @default.
- W2030607403 cites W1530801197 @default.
- W2030607403 cites W1586550223 @default.
- W2030607403 cites W1599491542 @default.
- W2030607403 cites W1968763251 @default.
- W2030607403 cites W1976607726 @default.
- W2030607403 cites W1976701520 @default.
- W2030607403 cites W1998089380 @default.
- W2030607403 cites W2005234047 @default.
- W2030607403 cites W2033505230 @default.
- W2030607403 cites W2041358582 @default.
- W2030607403 cites W2052454193 @default.
- W2030607403 cites W2063683060 @default.
- W2030607403 cites W2069439613 @default.
- W2030607403 cites W2077945132 @default.
- W2030607403 cites W2159859020 @default.
- W2030607403 cites W2412910126 @default.
- W2030607403 cites W2426438349 @default.
- W2030607403 doi "https://doi.org/10.1111/j.1432-1033.1976.tb10825.x" @default.
- W2030607403 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/976263" @default.
- W2030607403 hasPublicationYear "1976" @default.
- W2030607403 type Work @default.
- W2030607403 sameAs 2030607403 @default.
- W2030607403 citedByCount "25" @default.
- W2030607403 crossrefType "journal-article" @default.
- W2030607403 hasAuthorship W2030607403A5002034237 @default.
- W2030607403 hasAuthorship W2030607403A5006078287 @default.
- W2030607403 hasAuthorship W2030607403A5024944429 @default.
- W2030607403 hasAuthorship W2030607403A5028108508 @default.
- W2030607403 hasAuthorship W2030607403A5071364492 @default.
- W2030607403 hasConcept C161790260 @default.
- W2030607403 hasConcept C164361826 @default.
- W2030607403 hasConcept C181199279 @default.
- W2030607403 hasConcept C185592680 @default.
- W2030607403 hasConcept C197957613 @default.
- W2030607403 hasConcept C2776317432 @default.
- W2030607403 hasConcept C2776568683 @default.
- W2030607403 hasConcept C2778460671 @default.
- W2030607403 hasConcept C2778689049 @default.
- W2030607403 hasConcept C2779201268 @default.
- W2030607403 hasConcept C2780902042 @default.
- W2030607403 hasConcept C41183919 @default.
- W2030607403 hasConcept C55493867 @default.
- W2030607403 hasConcept C71240020 @default.
- W2030607403 hasConcept C71315377 @default.
- W2030607403 hasConcept C75520062 @default.
- W2030607403 hasConceptScore W2030607403C161790260 @default.
- W2030607403 hasConceptScore W2030607403C164361826 @default.
- W2030607403 hasConceptScore W2030607403C181199279 @default.
- W2030607403 hasConceptScore W2030607403C185592680 @default.
- W2030607403 hasConceptScore W2030607403C197957613 @default.
- W2030607403 hasConceptScore W2030607403C2776317432 @default.
- W2030607403 hasConceptScore W2030607403C2776568683 @default.
- W2030607403 hasConceptScore W2030607403C2778460671 @default.
- W2030607403 hasConceptScore W2030607403C2778689049 @default.
- W2030607403 hasConceptScore W2030607403C2779201268 @default.
- W2030607403 hasConceptScore W2030607403C2780902042 @default.
- W2030607403 hasConceptScore W2030607403C41183919 @default.
- W2030607403 hasConceptScore W2030607403C55493867 @default.
- W2030607403 hasConceptScore W2030607403C71240020 @default.
- W2030607403 hasConceptScore W2030607403C71315377 @default.
- W2030607403 hasConceptScore W2030607403C75520062 @default.
- W2030607403 hasIssue "2" @default.
- W2030607403 hasLocation W20306074031 @default.
- W2030607403 hasLocation W20306074032 @default.
- W2030607403 hasOpenAccess W2030607403 @default.
- W2030607403 hasPrimaryLocation W20306074031 @default.
- W2030607403 hasRelatedWork W134642770 @default.
- W2030607403 hasRelatedWork W1953994264 @default.
- W2030607403 hasRelatedWork W2046311107 @default.
- W2030607403 hasRelatedWork W2049887237 @default.
- W2030607403 hasRelatedWork W2053583806 @default.
- W2030607403 hasRelatedWork W2069715420 @default.
- W2030607403 hasRelatedWork W2071151402 @default.
- W2030607403 hasRelatedWork W2167441450 @default.
- W2030607403 hasRelatedWork W2170874906 @default.
- W2030607403 hasRelatedWork W2322885984 @default.
- W2030607403 hasVolume "68" @default.
- W2030607403 isParatext "false" @default.
- W2030607403 isRetracted "false" @default.
- W2030607403 magId "2030607403" @default.
- W2030607403 workType "article" @default.