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- W2030616303 abstract "Summary When genes for flower color and other phenotypic characteristics are present in a Heterozygous condition in Tradescantia plants, they can be used as genetic markers for the study of induced mutation. The procedure used is to expose cuttings containing young flower buds to chemical or physical mutagens. 1 to 3 weeks later the resultant mutant pink or colorless cells can be seen in mature flowers, and after relatively simple scoring procedures somatic mutation rates can be calculated. Dose-response curves for ethyl methanesulfonate (EMS) and 1,2-dibromoethane (DBE) were obtained and compared with existing X-ray dose-response curves. Two clones, 02 and 4430 were exposed to known levels (from 5–250 ppm) of EMS in gaseous form for periods of from 1–19 h. After exposure to X-rays pink mutation rates showed similar sensitivities for both clones but the rate for colorless was much higher for clone 4430 and the slope much steeper. After treatment with EMS both colorless and pink rates were appreciably higher for clone 4430 than for clone 02. Preliminary data show similar differences in clonal sensitivity following exposures to DBE. The large differences in mutation rates between loci and between clones after chemical and physical mutagen treatments are not understood at present. Possibly differences in mutation rates between the two clones after EMS treatment may be associated with a larger amount of heterochromatin or a greater number of SAT-chromosomes in clone 4430. The genetic basis for the phenotypic changes (pink and colorless) is not definitely known but may be associated with chromosome breakage, gene mutation, chromosome non-disjunction, or somatic crossing over." @default.
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- W2030616303 date "1974-08-01" @default.
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- W2030616303 title "Comparison of somatic mutation rates induced in tradescantia by chemical and physical mutagens" @default.
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- W2030616303 doi "https://doi.org/10.1016/s0027-5107(74)80024-2" @default.
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