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• W2030622120 abstract "The UGT1A1 promoter contains a (TA)n repeat polymorphism. The 7 repeat allele is associated with decreased enzyme activity and patients homozygous for this allele treated with irinotecan may experience life-threatening toxicity. Here, we have compared three methods [DNA sequencing, fragment analysis, and the Invader assay (Third Wave Technologies)] for genotyping this polymorphism.All of the DNA samples (n=119) had concordant genotype calls between the sequencing and size-based methods. The Invader method was also concordant if the genotypes were 6/6, 6/7, or 7/7. Both the size-based method and the Invader method had straightforward data analysis, while interpretation of the sequencing results was occasionally more challenging. The Invader method required more concentrated DNA for analysis, was more expensive, and had a limited genotyping spectrum.All three methods were valuable for genotyping the UGT1A1 (TA)n repeat, with the sequencing and size-based assays having the fewest drawbacks." @default.
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• W2030622120 date "2007-06-01" @default.
• W2030622120 modified "2023-09-25" @default.
• W2030622120 title "Comparison of three methods for genotyping the UGT1A1 (TA)n repeat polymorphism" @default.
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• W2030622120 doi "https://doi.org/10.1016/j.clinbiochem.2007.03.007" @default.
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