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• W2030639710 abstract "We synthesized the following four new peptide substrates, Suc−Phe−Leu−pNA, Suc−Phe−Leu−NMec, Suc−Phe−Leu−ONPh, and Pht−Phe−Leu−pNA, and we applied the proton inventory method to their hydrolysis by papain. Useful relationships between the rate constants of the catalytic reaction have been established and contributed to the elucidation of the hydrolytic mechanism of papain. For all amide substrates, the parameter KS and the rate constants k1, k-1, and k2 were estimated. Moreover, it was found that kcat/Km = k1 for all four substrates, while two exchangeable hydrogenic sites, one in the ground state and another in the transition state, generate an inverse isotope effect during the reaction governed by this parameter. The proton inventories of both k2 and k3 are essentially linear, whatever the acyl moiety and/or the leaving group of the substrate. The proton inventories of KS are also essentially linear for all amide substrates, while the observed large isotope effect of about 3 to 9 originates from a single hydrogenic site in the product state. This latter, in agreement to both the small transition state fractionation factors found for kcat/Km (or k1) and the unit ground-state fractionation factors found for k2, argues for the formation of a tetrahedral adduct during the reaction governed by the k1 parameter. Furthermore, papain acts as a one-proton catalyst during acylation or deacylation, both of which proceed through similar concerted reaction pathways, where a nucleophilic attack is accompanied by the movement of one proton." @default.
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• W2030639710 date "2001-03-06" @default.
• W2030639710 modified "2023-09-30" @default.
• W2030639710 title "Insight into the Catalysis of Hydrolysis of Four Newly Synthesized Substrates by Papain: A Proton Inventory Study" @default.
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• W2030639710 doi "https://doi.org/10.1021/bi001615b" @default.
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