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• W2030642011 abstract "In HEK 293 cells expressing the human dopamine transporter (DAT), a 10-min incubation with 10 microM cocaine followed by extensive washing resulted in a 30% increase in [3H]dopamine (DA) uptake as well as an increase in cell surface DAT in biotinylation experiments. Consistent with this novel regulation, [3H]DA uptake into synaptosomes prepared from the nucleus accumbens of rats sacrificed 30 min after a single cocaine injection (30 mg/kg) was significantly increased compared to controls (56% increase in V(max), no change in K(m)). In addition, DA clearance in the striatum of anesthetized rats was increased after local application of a low (3 pmol) but not high (65 pmol) dose of cocaine, presumably as a result of mobilization of DAT to the cell surface. Cocaine-induced increases in cell surface expression of DAT and associated changes in DA clearance represent a novel mechanism that may play a role in its addictive properties." @default.
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• W2030642011 date "2002-02-01" @default.
• W2030642011 modified "2023-10-18" @default.
• W2030642011 title "Cocaine Increases Dopamine Uptake and Cell Surface Expression of Dopamine Transporters" @default.
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• W2030642011 doi "https://doi.org/10.1006/bbrc.2002.6384" @default.
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