Matches in SemOpenAlex for { <https://semopenalex.org/work/W2030652009> ?p ?o ?g. }
Showing items 1 to 100 of
100
with 100 items per page.
- W2030652009 endingPage "37" @default.
- W2030652009 startingPage "26" @default.
- W2030652009 abstract "High-energy collisional dissociation (HCD) is an efficient peptide fragmentation method that is widely used in Orbitrap mass spectrometers. A greater understanding of HCD fragmentation patterns would benefit the development of proteomic data analysis algorithms. In this study, b and y ion fragmentation patterns and residue-specific cleavage effects in HCD mode were statistically characterized on a LTQ Orbitrap Velos mass spectrometer. We compared HCD and CID spectra collected in an Orbitrap for the same doubly and triply charged tryptic peptides. Our analytical results revealed novel statistical features of HCD spectra. The intensity of y ions reached a maximum in the 60-70% and 40-50% relative mass bins of HCD spectra from doubly and triply charged peptides, respectively. The HCD mode showed a slight preference for generating y ions with lower charges than did CID mode. Singly charged fragment ions dominated the five fragment ions with the highest intensity in HCD spectra. Hydrophobic residues for b ions were the primary differences in cleavage selectivity between the two modes, while residues for y ions showed a similar cleavage preference. These results will assist with the development of database search engines and the design of proper transitions for targeted proteomic analysis.Orbitrap mass spectrometry is becoming the popular instrument for proteomic analysis, and HCD mode is becoming the main analysis mode for its high resolution and high sensitivity. This study characterizes the features of HCD spectrum by comparing with CID mode on LTQ Velos Orbitrap. The patterns of b and y ions in HCD and CID modes were systematically compared for the first time, including the charge state, ion frequency and intensity and cleavage selectivity. These results will help develop database search engines and design proper transitions for targeted proteomic analysis in the future." @default.
- W2030652009 created "2016-06-24" @default.
- W2030652009 creator A5001712204 @default.
- W2030652009 creator A5022802322 @default.
- W2030652009 creator A5085790081 @default.
- W2030652009 date "2014-09-01" @default.
- W2030652009 modified "2023-10-09" @default.
- W2030652009 title "Statistical characterization of HCD fragmentation patterns of tryptic peptides on an LTQ Orbitrap Velos mass spectrometer" @default.
- W2030652009 cites W1969569756 @default.
- W2030652009 cites W1978640976 @default.
- W2030652009 cites W2000798961 @default.
- W2030652009 cites W2005716310 @default.
- W2030652009 cites W2007235785 @default.
- W2030652009 cites W2011503307 @default.
- W2030652009 cites W2011974256 @default.
- W2030652009 cites W2016056088 @default.
- W2030652009 cites W2018247248 @default.
- W2030652009 cites W2019640782 @default.
- W2030652009 cites W2030318299 @default.
- W2030652009 cites W2033492963 @default.
- W2030652009 cites W2037199116 @default.
- W2030652009 cites W2043594346 @default.
- W2030652009 cites W2049666620 @default.
- W2030652009 cites W2051644573 @default.
- W2030652009 cites W2053943711 @default.
- W2030652009 cites W2055934103 @default.
- W2030652009 cites W2060034851 @default.
- W2030652009 cites W2073401456 @default.
- W2030652009 cites W2078598194 @default.
- W2030652009 cites W2087212852 @default.
- W2030652009 cites W2095650214 @default.
- W2030652009 cites W2115927224 @default.
- W2030652009 cites W2117903500 @default.
- W2030652009 cites W2139208986 @default.
- W2030652009 cites W744677427 @default.
- W2030652009 doi "https://doi.org/10.1016/j.jprot.2014.06.012" @default.
- W2030652009 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24981973" @default.
- W2030652009 hasPublicationYear "2014" @default.
- W2030652009 type Work @default.
- W2030652009 sameAs 2030652009 @default.
- W2030652009 citedByCount "14" @default.
- W2030652009 countsByYear W20306520092015 @default.
- W2030652009 countsByYear W20306520092016 @default.
- W2030652009 countsByYear W20306520092017 @default.
- W2030652009 countsByYear W20306520092018 @default.
- W2030652009 countsByYear W20306520092019 @default.
- W2030652009 countsByYear W20306520092020 @default.
- W2030652009 countsByYear W20306520092021 @default.
- W2030652009 countsByYear W20306520092022 @default.
- W2030652009 countsByYear W20306520092023 @default.
- W2030652009 crossrefType "journal-article" @default.
- W2030652009 hasAuthorship W2030652009A5001712204 @default.
- W2030652009 hasAuthorship W2030652009A5022802322 @default.
- W2030652009 hasAuthorship W2030652009A5085790081 @default.
- W2030652009 hasConcept C111919701 @default.
- W2030652009 hasConcept C113196181 @default.
- W2030652009 hasConcept C145148216 @default.
- W2030652009 hasConcept C162356407 @default.
- W2030652009 hasConcept C178790620 @default.
- W2030652009 hasConcept C185592680 @default.
- W2030652009 hasConcept C191015642 @default.
- W2030652009 hasConcept C2780841973 @default.
- W2030652009 hasConcept C40325409 @default.
- W2030652009 hasConcept C41008148 @default.
- W2030652009 hasConcept C43617362 @default.
- W2030652009 hasConceptScore W2030652009C111919701 @default.
- W2030652009 hasConceptScore W2030652009C113196181 @default.
- W2030652009 hasConceptScore W2030652009C145148216 @default.
- W2030652009 hasConceptScore W2030652009C162356407 @default.
- W2030652009 hasConceptScore W2030652009C178790620 @default.
- W2030652009 hasConceptScore W2030652009C185592680 @default.
- W2030652009 hasConceptScore W2030652009C191015642 @default.
- W2030652009 hasConceptScore W2030652009C2780841973 @default.
- W2030652009 hasConceptScore W2030652009C40325409 @default.
- W2030652009 hasConceptScore W2030652009C41008148 @default.
- W2030652009 hasConceptScore W2030652009C43617362 @default.
- W2030652009 hasFunder F4320321001 @default.
- W2030652009 hasFunder F4320321769 @default.
- W2030652009 hasFunder F4320322919 @default.
- W2030652009 hasLocation W20306520091 @default.
- W2030652009 hasLocation W20306520092 @default.
- W2030652009 hasOpenAccess W2030652009 @default.
- W2030652009 hasPrimaryLocation W20306520091 @default.
- W2030652009 hasRelatedWork W1984330945 @default.
- W2030652009 hasRelatedWork W1986115730 @default.
- W2030652009 hasRelatedWork W2023698907 @default.
- W2030652009 hasRelatedWork W2026642978 @default.
- W2030652009 hasRelatedWork W2078110256 @default.
- W2030652009 hasRelatedWork W2166581334 @default.
- W2030652009 hasRelatedWork W2313956144 @default.
- W2030652009 hasRelatedWork W2371154968 @default.
- W2030652009 hasRelatedWork W268201517 @default.
- W2030652009 hasRelatedWork W2902323604 @default.
- W2030652009 hasVolume "109" @default.
- W2030652009 isParatext "false" @default.
- W2030652009 isRetracted "false" @default.
- W2030652009 magId "2030652009" @default.
- W2030652009 workType "article" @default.