FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2030725344> ?p ?o ?g. }

• W2030725344 endingPage "6779" @default.
• W2030725344 startingPage "6769" @default.
• W2030725344 abstract "In yeast, the inositolphosphorylceramides mostly contain C26:0 fatty acids. Inositolphosphorylceramides were considered to be important for viability because the inositolphosphorylceramide synthase AUR1 is essential. However, lcb1Δ cells, unable to make sphingoid bases and inositolphosphorylceramides, are viable if they harbor SLC1-1, a gain of function mutation in the 1-acyl-glycerol-3-phosphate acyltransferase SLC1. SLC1-1 allows the incorporation of C26:0 fatty acids into phosphatidylinositol (PI), thus generating PI″, an abnormal, C26-containing PI, presumably acting as surrogate for inositolphosphorylceramide. Here we show that the lethality of the simultaneous deletion of the known ceramide synthases LAG1/LAC1/LIP1 and YPC1/YDC1 can be rescued by the expression of SLC1-1 or the overexpression of AUR1. Moreover, lag1Δ lac1Δ ypc1Δ ydc1Δ (4Δ) quadruple mutants have been reported to be viable in certain genetic backgrounds but to still make some abnormal uncharacterized inositol-containing sphingolipids. Indeed, we find that 4Δ quadruple mutants make substantial amounts of unphysiological inositolphosphorylphytosphingosines but that they also still make small amounts of normal inositolphosphorylceramides. Moreover, 4Δ strains incorporate exogenously added sphingoid bases into inositolphosphorylceramides, indicating that these cells still possess an unknown pathway allowing the synthesis of ceramides. 4Δ cells also still add quite normal amounts of ceramides to glycosylphosphatidylinositol anchors. Synthesis of inositolphosphorylceramides and inositolphosphorylphytosphingosines is operated by Aur1p and is essential for growth of all 4Δ cells unless they contain SLC1-1. PI″, however, is made without the help of Aur1p. Furthermore, mannosylation of PI″ is required for the survival of sphingolipid-deficient strains, which depend on SLC1-1. In contrast to lcb1Δ SLC1-1, 4Δ SLC1-1 cells grow at 37 °C but remain thermosensitive at 44 °C." @default.
• W2030725344 created "2016-06-24" @default.
• W2030725344 creator A5012580074 @default.
• W2030725344 creator A5018036156 @default.
• W2030725344 creator A5052363755 @default.
• W2030725344 creator A5074499137 @default.
• W2030725344 creator A5084454705 @default.
• W2030725344 date "2011-02-01" @default.
• W2030725344 modified "2023-10-16" @default.
• W2030725344 title "Yeast Cells Lacking All Known Ceramide Synthases Continue to Make Complex Sphingolipids and to Incorporate Ceramides into Glycosylphosphatidylinositol (GPI) Anchors" @default.
• W2030725344 cites W1497947649 @default.
• W2030725344 cites W1582748592 @default.
• W2030725344 cites W1598208661 @default.
• W2030725344 cites W1600394682 @default.
• W2030725344 cites W1775271799 @default.
• W2030725344 cites W1973800195 @default.
• W2030725344 cites W1974618932 @default.
• W2030725344 cites W1982986969 @default.
• W2030725344 cites W1984382879 @default.
• W2030725344 cites W1984954439 @default.
• W2030725344 cites W1992718750 @default.
• W2030725344 cites W1996252412 @default.
• W2030725344 cites W1996653295 @default.
• W2030725344 cites W2000456081 @default.
• W2030725344 cites W2016874659 @default.
• W2030725344 cites W2024830340 @default.
• W2030725344 cites W2029002443 @default.
• W2030725344 cites W2032850782 @default.
• W2030725344 cites W2035954192 @default.
• W2030725344 cites W2040025772 @default.
• W2030725344 cites W2043476857 @default.
• W2030725344 cites W2047083922 @default.
• W2030725344 cites W2051691075 @default.
• W2030725344 cites W2051731160 @default.
• W2030725344 cites W2054773029 @default.
• W2030725344 cites W2055425310 @default.
• W2030725344 cites W2057704289 @default.
• W2030725344 cites W2062612796 @default.
• W2030725344 cites W2064379250 @default.
• W2030725344 cites W2066160116 @default.
• W2030725344 cites W2071541941 @default.
• W2030725344 cites W2076635184 @default.
• W2030725344 cites W2097323575 @default.
• W2030725344 cites W2099166687 @default.
• W2030725344 cites W2099521932 @default.
• W2030725344 cites W2100088060 @default.
• W2030725344 cites W2100285359 @default.
• W2030725344 cites W2102238970 @default.
• W2030725344 cites W2112882175 @default.
• W2030725344 cites W2116122989 @default.
• W2030725344 cites W2118132797 @default.
• W2030725344 cites W2120328280 @default.
• W2030725344 cites W2126338409 @default.
• W2030725344 cites W2127220887 @default.
• W2030725344 cites W2130264382 @default.
• W2030725344 cites W2133779340 @default.
• W2030725344 cites W2135752763 @default.
• W2030725344 cites W2137158186 @default.
• W2030725344 cites W2144752869 @default.
• W2030725344 cites W2147276807 @default.
• W2030725344 cites W2154079436 @default.
• W2030725344 cites W2162376274 @default.
• W2030725344 cites W2164236208 @default.
• W2030725344 cites W2166444997 @default.
• W2030725344 cites W2167603249 @default.
• W2030725344 cites W2170108898 @default.
• W2030725344 cites W2184043735 @default.
• W2030725344 cites W266743478 @default.
• W2030725344 doi "https://doi.org/10.1074/jbc.m110.176875" @default.
• W2030725344 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3057787" @default.
• W2030725344 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21173150" @default.
• W2030725344 hasPublicationYear "2011" @default.
• W2030725344 type Work @default.
• W2030725344 sameAs 2030725344 @default.
• W2030725344 citedByCount "18" @default.
• W2030725344 countsByYear W20307253442012 @default.
• W2030725344 countsByYear W20307253442013 @default.
• W2030725344 countsByYear W20307253442014 @default.
• W2030725344 countsByYear W20307253442015 @default.
• W2030725344 countsByYear W20307253442016 @default.
• W2030725344 countsByYear W20307253442017 @default.
• W2030725344 countsByYear W20307253442018 @default.
• W2030725344 countsByYear W20307253442019 @default.
• W2030725344 countsByYear W20307253442021 @default.
• W2030725344 crossrefType "journal-article" @default.
• W2030725344 hasAuthorship W2030725344A5012580074 @default.
• W2030725344 hasAuthorship W2030725344A5018036156 @default.
• W2030725344 hasAuthorship W2030725344A5052363755 @default.
• W2030725344 hasAuthorship W2030725344A5074499137 @default.
• W2030725344 hasAuthorship W2030725344A5084454705 @default.
• W2030725344 hasBestOaLocation W20307253441 @default.
• W2030725344 hasConcept C104317684 @default.
• W2030725344 hasConcept C14036430 @default.
• W2030725344 hasConcept C143065580 @default.
• W2030725344 hasConcept C170493617 @default.
• W2030725344 hasConcept C181199279 @default.
• W2030725344 hasConcept C190283241 @default.
• W2030725344 hasConcept C2775970874 @default.

• next