FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2030741212> ?p ?o ?g. }

• W2030741212 abstract "Internodal stem segments of 2-4-month-old Quercus rubra L. seedlings were cultured on Murashige and Skoog agar medium containing NAA and BA. Structures, termed organoids, were initiated from callus on medium containing 5 mg/l NAA and 0.1 mg/l BA. Organoids consisted of parenchymatous cells with a distinct epidermis and a continuous vascular system. Growth occurred principally by cell division throughout the parenchymatous tissue resulting in a variety of morphological shapes. Although no organized shoot meristems were observed, normal roots were produced; such roots were connected to the vascular system of the organoid. RED OAKS (Quercus rubra L.), major hardwoods of northern midwest forests, valued for their aesthetic and timber qualities, are succumbing to oak wilt induced by the fungus Ceratocystis fagacearum (Bretz) Hunt. Death of infected trees results from the formation of tyloses in the vessels of the xylem (Struckmeyer et al., 1954). Resistance to oak wilt in natural populations of red oak has been observed in Wisconsin (R. D. Durbin, pers. commun.). However, no practical means to increase the number of trees showing resistance to the disease are presently available because seedlings are highly heterozygous and conventional asexual propagation practices are unsuccessful (Skinner, 1952; Flemer, 1962; Jaynes, 1964). In research designed to adapt microculture techniques to the propagation of wilt-resistant red oaks, an apparent anomaly in the development of differentiated shoots from calli was observed. These structures, termed organoids, are described in detail in this report. MATERIALS AND METHODS-Stems were excised from 2-4-month-old, greenhouse-grown red oak seedlings, surface sterilized by dipping in 70% ethanol for 5 seconds, and then transferred to 10% chlorine bleach for 5 min. After three successive rinses in sterile, distilled water, the stems were cut into internodal segments approximately 4 mm in length. The excised tissue 1 Received for publication 9 October 1978; revision accepted 7 May 1979. Research supported by the College of Agricultural and Life Sciences, by the Graduate School, and by the UniversityIndustry Research Program, University of Wisconsin, Madison. The authors express their appreciation to Dr. R. D. Durbin for plant material and invaluable discussions. was cultured on 10 ml of Murashige and Skoog (MS) agar medium (1962) supplemented with 0, 0.01, 0.1, 1, or 5 mg/l benzyladenine (BA) and 0, 0.01, 1, 3, 5, or 10 mg/l a-naphthaleneacetic acid (NAA) in 30-ml glass jars sealed with Parafilm-M. The jars were placed under a 16-hr photoperiod of cool-white fluorescent light at 5 nE! cm2/sec for the 400-700 nm wave band. Tissue for microscopic observation was fixed in the Allen-Bouin formula (Berlyn and Miksche, 1976) for at least 40 hr, dehydrated in a tertiary butyl alcohol series, and infiltrated and embedded in Paraplast. Sections were cut 10 ,um in thickness on a rotary microtome, mounted on glass slides with Haupt's adhesive, and stained with safranin-crystal violet-light green SF (Gerlach, 1969). RESULTS-Stem segments were placed longitudinally on MS agar medium containing all combinations of BA and NAA. After approximately 7 weeks, isolated callus cultures produced anatomically unique structures termed (Fig. 1). Organoids formed predominately on calli grown on medium containing 5 mg/l NAA and 0.1 mg/l BA. Although the incidence of organoid initiation was low, no attempt to optimize organoid production was made. Organoids were initiated on callus attached to the original stem segment as well as on subcultured callus. The term is used because of the presence of three tissue systems inherent in plant organs, namely, the dermal, the vascular, and the ground tissue (Esau, 1977). However, these organoids differ from true organs in that the former do not appear to originate from organized meristems. Anatomical studies revealed that organoids were initiated from the periphery of calli (Fig. 2)." @default.
• W2030741212 created "2016-06-24" @default.
• W2030741212 creator A5034940770 @default.
• W2030741212 creator A5039350300 @default.
• W2030741212 creator A5056009530 @default.
• W2030741212 date "1979-09-01" @default.
• W2030741212 modified "2023-09-24" @default.
• W2030741212 title "PRODUCTION OF ANOMALOUS STRUCTURES IN QUERCUS RUBRA L. CALLUS CULTURES" @default.
• W2030741212 cites W1996723114 @default.
• W2030741212 cites W2024556021 @default.
• W2030741212 cites W2028470234 @default.
• W2030741212 cites W2073843342 @default.
• W2030741212 doi "https://doi.org/10.1002/j.1537-2197.1979.tb06313.x" @default.
• W2030741212 hasPublicationYear "1979" @default.
• W2030741212 type Work @default.
• W2030741212 sameAs 2030741212 @default.
• W2030741212 citedByCount "10" @default.
• W2030741212 countsByYear W20307412122017 @default.
• W2030741212 crossrefType "journal-article" @default.
• W2030741212 hasAuthorship W2030741212A5034940770 @default.
• W2030741212 hasAuthorship W2030741212A5039350300 @default.
• W2030741212 hasAuthorship W2030741212A5056009530 @default.
• W2030741212 hasConcept C126021549 @default.
• W2030741212 hasConcept C187820883 @default.
• W2030741212 hasConcept C21410773 @default.
• W2030741212 hasConcept C49799701 @default.
• W2030741212 hasConcept C59822182 @default.
• W2030741212 hasConcept C86803240 @default.
• W2030741212 hasConcept C9549007 @default.
• W2030741212 hasConceptScore W2030741212C126021549 @default.
• W2030741212 hasConceptScore W2030741212C187820883 @default.
• W2030741212 hasConceptScore W2030741212C21410773 @default.
• W2030741212 hasConceptScore W2030741212C49799701 @default.
• W2030741212 hasConceptScore W2030741212C59822182 @default.
• W2030741212 hasConceptScore W2030741212C86803240 @default.
• W2030741212 hasConceptScore W2030741212C9549007 @default.
• W2030741212 hasFunder F4320332126 @default.
• W2030741212 hasLocation W20307412121 @default.
• W2030741212 hasOpenAccess W2030741212 @default.
• W2030741212 hasPrimaryLocation W20307412121 @default.
• W2030741212 hasRelatedWork W1956366137 @default.
• W2030741212 hasRelatedWork W1997489795 @default.
• W2030741212 hasRelatedWork W1998553666 @default.
• W2030741212 hasRelatedWork W2000291935 @default.
• W2030741212 hasRelatedWork W2008963988 @default.
• W2030741212 hasRelatedWork W2015367949 @default.
• W2030741212 hasRelatedWork W2015559742 @default.
• W2030741212 hasRelatedWork W2021022991 @default.
• W2030741212 hasRelatedWork W2024556021 @default.
• W2030741212 hasRelatedWork W2048520468 @default.
• W2030741212 hasRelatedWork W2056468478 @default.
• W2030741212 hasRelatedWork W2270731821 @default.
• W2030741212 hasRelatedWork W2312668863 @default.
• W2030741212 hasRelatedWork W2397675363 @default.
• W2030741212 hasRelatedWork W2416985984 @default.
• W2030741212 hasRelatedWork W2592195440 @default.
• W2030741212 hasRelatedWork W3206270851 @default.
• W2030741212 hasRelatedWork W67734094 @default.
• W2030741212 hasRelatedWork W2184428635 @default.
• W2030741212 hasRelatedWork W2344303317 @default.
• W2030741212 isParatext "false" @default.
• W2030741212 isRetracted "false" @default.
• W2030741212 magId "2030741212" @default.
• W2030741212 workType "article" @default.