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• W2030754598 startingPage "C1636" @default.
• W2030754598 abstract "We used the short-circuit current ( I sc ) technique to investigate the effects of the isoflavone genistein on the electrogenic Cl − secretion of the mouse jejunum. Genistein stimulated a sustained increase in I sc that was dose dependent. Bumetanide inhibited 76 ± 5% of the genistein-stimulated I sc consistent with activation of Cl − secretion. Genistein failed to stimulate I sc following maximal activation of the cAMP pathway by forskolin. In addition, forskolin had a reduced effect on I sc of the mouse jejunum in the presence of genistein. Glibenclamide, a blocker of CFTR, eliminated the genistein-stimulated increase of I sc and reduced the forskolin-activated I sc . Clotrimazole, a Ca 2+ -activated K + channel blocker, failed to reduce the genistein-stimulated I sc . Vanadate, a blocker of tyrosine-dependent phosphatases, reduced the genistein-activated I sc . Tyrphostin A23, a tyrosine kinase inhibitor, reduced basal I sc , after which genistein failed to stimulate I sc . These data suggest that genistein activated a sustained Cl − secretory response of the mouse jejunum and that the effect of genistein was via a tyrosine-dependent phosphorylation pathway." @default.
• W2030754598 created "2016-06-24" @default.
• W2030754598 creator A5017575646 @default.
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• W2030754598 date "2004-12-01" @default.
• W2030754598 modified "2023-09-27" @default.
• W2030754598 title "Genistein stimulates electrogenic Cl⁻secretion in mouse jejunum" @default.
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• W2030754598 doi "https://doi.org/10.1152/ajpcell.00236.2003" @default.
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