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- W2030771572 abstract "Capillary electrophoresis has been used to separate and quantitate glyphosate and its major metabolite, (aminomethyl)phosphonic acid (AMPA), in serum. The two compounds, after derivatization with p-toluenesulphonyl chloride, were clearly separated with 0.1 M boric acid—sodium hydroxide buffer (pH 9.6) containing 10% methanol. The separation was completed within 15 min at an applied potential of 30 kV. Calibration curves for the assay were linear over both the lower (0.5–10 μg/ml) and the higher (10–100 μg/ml) concentration ranges. The within-run and day-to-day coefficients of variation of peak area were 1.4–4.4 and 4.4–8.5%, respectively, for glyphosate and 1.8–2.9 and 1.8–2.9%, respectively, for AMPA. The within-run and day-to-day precisions of the migration time for both compounds were less than 1.8% and less than 2.5%, respectively. The detection limit of both derivatives was 0.1 μg/ml in spiked sera, and the recoveries of glyphosate and AMPA were 87.9–88.8 and 78.4–86.9%, respectively. In this study, the reproducibility and the effect of pH changes on the electropherograms were especially examined." @default.
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- W2030771572 date "1991-12-01" @default.
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- W2030771572 title "Determination of glyphosate and its metabolite, (aminomethyl)phosphonic acid, in serum using capillary electrophoresis" @default.
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- W2030771572 doi "https://doi.org/10.1016/0378-4347(91)80463-m" @default.
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