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- W2030804237 abstract "Abstract The investigation of the role of ceramides in maintaining the barrier function of stratum corneum (SC) has brought about the need of specific analytical methods. The present paper describes a combination of high-performance thin-layer chromatography (HPTLC) using automated multiple development, which was modified for semi-preparative purposes, with subsequent liquid chromatography–electrospray mass spectrometry for the profiling of ceramides. The fractionation of complex lipid extracts with the help of thin-layer chromatography facilitates a specific, sensitive and reliable analysis. Characteristic patterns of the molecular mass distribution in different ceramide fractions are easily obtainable. Additional electrospray tandem mass spectrometry using an ion trap mass spectrometer provides fragment ions which indicate the sphingoid base as well as the fatty acid moiety and therefore enables the exact assignment of ceramide structures. Furthermore, a method of liquid chromatography–electrospray mass spectrometry for the quantitation of cholesterol-3-sulfate is presented." @default.
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- W2030804237 date "2000-08-01" @default.
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- W2030804237 title "Profiling of human stratum corneum ceramides by liquid chromatography–electrospray mass spectrometry" @default.
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- W2030804237 doi "https://doi.org/10.1016/s0003-2670(00)00955-7" @default.
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