FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2030822571> ?p ?o ?g. }

• W2030822571 endingPage "113" @default.
• W2030822571 startingPage "106" @default.
• W2030822571 abstract "Flavivirus NS2B-NS3 proteases are associated with neurovirulence, becoming an important target for insight into the virus-induced pathogenesis. In this study, a phage-displayed human brain cDNA library was used to detect possible interaction between brain proteins and the Japanese encephalitis virus (JEV) NS2B-NS3 protease. After six rounds of biopanning, eight high-affinity NS2B-NS3 protease-interacting phages were identified. Identified NS2B-NS3 protease-interacting brain proteins contained several repeats of the consensus motifs E(R/K)(R/K)K and G(R/K)(R/K) with the dibasic residues, being similar to the conserved cleavage sites among flavivirus proteases. In addition, three identified brain proteins (phage-24, 34, and 44) were predicted as the domain of trypsin inhibitor and basic region leucine zipper (bZIP) using the SMART genome search. Immunoprecipitation and cleavage of two brain fusion proteins (phage-24 and phage-46) by the NS2B-NS3 protease confirmed the specific interaction between identified brain proteins and the JEV NS2B-NS3 protease. Fluorogenic peptide substrate assays revealed dose-manner inhibitory effects of these two brain fusion proteins on the trans-cleavage activity of NS2B-NS3 protease. Moreover, in vitro signaling pathway assay revealed that the JEV NS2B-NS3 protease significantly inhibited the signaling pathway of activator protein 1(AP1), a member of the bZIP family. Our results provide an insight into the protein interaction network of the JEV NS2B-NS3 protease in human brain." @default.
• W2030822571 created "2016-06-24" @default.
• W2030822571 creator A5025517741 @default.
• W2030822571 creator A5027251927 @default.
• W2030822571 creator A5028741023 @default.
• W2030822571 creator A5075048093 @default.
• W2030822571 date "2006-03-01" @default.
• W2030822571 modified "2023-10-06" @default.
• W2030822571 title "Japanese encephalitis virus NS2B-NS3 protease binding to phage-displayed human brain proteins with the domain of trypsin inhibitor and basic region leucine zipper" @default.
• W2030822571 cites W1258517923 @default.
• W2030822571 cites W1844643319 @default.
• W2030822571 cites W1865324159 @default.
• W2030822571 cites W1984520500 @default.
• W2030822571 cites W1990482989 @default.
• W2030822571 cites W1993687095 @default.
• W2030822571 cites W1994219871 @default.
• W2030822571 cites W2002185589 @default.
• W2030822571 cites W2016400471 @default.
• W2030822571 cites W2016789574 @default.
• W2030822571 cites W2017278177 @default.
• W2030822571 cites W2018620137 @default.
• W2030822571 cites W2020942480 @default.
• W2030822571 cites W2027668719 @default.
• W2030822571 cites W2035650745 @default.
• W2030822571 cites W2036093440 @default.
• W2030822571 cites W2037279384 @default.
• W2030822571 cites W2070360032 @default.
• W2030822571 cites W2106494184 @default.
• W2030822571 cites W2142821747 @default.
• W2030822571 cites W2150029541 @default.
• W2030822571 cites W2158131682 @default.
• W2030822571 doi "https://doi.org/10.1016/j.virusres.2005.09.002" @default.
• W2030822571 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16289409" @default.
• W2030822571 hasPublicationYear "2006" @default.
• W2030822571 type Work @default.
• W2030822571 sameAs 2030822571 @default.
• W2030822571 citedByCount "15" @default.
• W2030822571 countsByYear W20308225712013 @default.
• W2030822571 countsByYear W20308225712014 @default.
• W2030822571 countsByYear W20308225712015 @default.
• W2030822571 countsByYear W20308225712016 @default.
• W2030822571 countsByYear W20308225712017 @default.
• W2030822571 countsByYear W20308225712021 @default.
• W2030822571 crossrefType "journal-article" @default.
• W2030822571 hasAuthorship W2030822571A5025517741 @default.
• W2030822571 hasAuthorship W2030822571A5027251927 @default.
• W2030822571 hasAuthorship W2030822571A5028741023 @default.
• W2030822571 hasAuthorship W2030822571A5075048093 @default.
• W2030822571 hasConcept C104317684 @default.
• W2030822571 hasConcept C123894998 @default.
• W2030822571 hasConcept C132379496 @default.
• W2030822571 hasConcept C153911025 @default.
• W2030822571 hasConcept C156860981 @default.
• W2030822571 hasConcept C159047783 @default.
• W2030822571 hasConcept C167625842 @default.
• W2030822571 hasConcept C181199279 @default.
• W2030822571 hasConcept C182220744 @default.
• W2030822571 hasConcept C186268636 @default.
• W2030822571 hasConcept C2522874641 @default.
• W2030822571 hasConcept C2776714187 @default.
• W2030822571 hasConcept C2777807008 @default.
• W2030822571 hasConcept C2779281246 @default.
• W2030822571 hasConcept C2780568436 @default.
• W2030822571 hasConcept C40767141 @default.
• W2030822571 hasConcept C55493867 @default.
• W2030822571 hasConcept C82553315 @default.
• W2030822571 hasConcept C83100098 @default.
• W2030822571 hasConcept C86803240 @default.
• W2030822571 hasConceptScore W2030822571C104317684 @default.
• W2030822571 hasConceptScore W2030822571C123894998 @default.
• W2030822571 hasConceptScore W2030822571C132379496 @default.
• W2030822571 hasConceptScore W2030822571C153911025 @default.
• W2030822571 hasConceptScore W2030822571C156860981 @default.
• W2030822571 hasConceptScore W2030822571C159047783 @default.
• W2030822571 hasConceptScore W2030822571C167625842 @default.
• W2030822571 hasConceptScore W2030822571C181199279 @default.
• W2030822571 hasConceptScore W2030822571C182220744 @default.
• W2030822571 hasConceptScore W2030822571C186268636 @default.
• W2030822571 hasConceptScore W2030822571C2522874641 @default.
• W2030822571 hasConceptScore W2030822571C2776714187 @default.
• W2030822571 hasConceptScore W2030822571C2777807008 @default.
• W2030822571 hasConceptScore W2030822571C2779281246 @default.
• W2030822571 hasConceptScore W2030822571C2780568436 @default.
• W2030822571 hasConceptScore W2030822571C40767141 @default.
• W2030822571 hasConceptScore W2030822571C55493867 @default.
• W2030822571 hasConceptScore W2030822571C82553315 @default.
• W2030822571 hasConceptScore W2030822571C83100098 @default.
• W2030822571 hasConceptScore W2030822571C86803240 @default.
• W2030822571 hasIssue "1-2" @default.
• W2030822571 hasLocation W20308225711 @default.
• W2030822571 hasLocation W20308225712 @default.
• W2030822571 hasOpenAccess W2030822571 @default.
• W2030822571 hasPrimaryLocation W20308225711 @default.
• W2030822571 hasRelatedWork W1820552472 @default.
• W2030822571 hasRelatedWork W1989503901 @default.
• W2030822571 hasRelatedWork W2004223206 @default.
• W2030822571 hasRelatedWork W2067819264 @default.
• W2030822571 hasRelatedWork W2074188269 @default.

• next