FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2030835318> ?p ?o ?g. }

• W2030835318 endingPage "25379" @default.
• W2030835318 startingPage "25373" @default.
• W2030835318 abstract "Abstract In mammals, folylpoly-γ-glutamate synthetase (FPGS) activity is found in any cell undergoing sustained proliferative phases, but this enzyme also displays a tissue-specific pattern of expression in differentiated tissues. It is now reported that the steady state levels of FPGS mRNA in normal and neoplastic cells reflect these patterns, supporting the concept that the control mechanisms underlying this distribution are transcriptional. To initiate an understanding of these interacting levels of control, we have determined the position and properties of the minimal FPGS promoter controlling transcription of the FPGS gene in human CEM leukemia cells, a line which expresses high levels of this enzyme and its mRNA. The TATA-less region immediately upstream of the major transcriptional start site previously mapped in human tumor cells, which includes several GC- and Y-boxes, functioned as a remarkably efficient promoter when used to drive expression of a luciferase reporter in transient expression studies in CEM cells. The minimal region of the FPGS promoter required for maximal transcriptional activation in CEM cells included the 80 base pairs over which the multiple transcriptional start sites were located, and the 43 base pairs immediately upstream. DNase I footprint analysis detected the binding of Sp1 at all seven of the consensus sites within the probe used, two of which are contained within the minimal promoter region. The several Sp1 sites immediately upstream of the first major transcriptional start activated transcription in Drosophilacells when cotransfected with an Sp1 construct, including those in the region which functioned as a minimal promoter in CEM cells. An additional region of the minimal promoter, situated between the two translational start codons of the FPGS gene, was bound by protein(s) from HeLa cell nuclear extracts. We conclude that transcription of the FPGS gene in CEM cells involves transactivation events over a limited upstream DNA sequence and that the FPGS promoter used in proliferating human leukemic cells has strong similarity to other TATA-less promoters that utilize tandem, closely spaced Sp1 sites to initiate transcription." @default.
• W2030835318 created "2016-06-24" @default.
• W2030835318 creator A5000404270 @default.
• W2030835318 creator A5032651935 @default.
• W2030835318 date "1997-10-01" @default.
• W2030835318 modified "2023-10-04" @default.
• W2030835318 title "Transcription of the Human Folylpoly-γ-glutamate Synthetase Gene" @default.
• W2030835318 cites W1595752904 @default.
• W2030835318 cites W1600598432 @default.
• W2030835318 cites W1667331074 @default.
• W2030835318 cites W1791228580 @default.
• W2030835318 cites W1972329286 @default.
• W2030835318 cites W1990450552 @default.
• W2030835318 cites W1990583841 @default.
• W2030835318 cites W2010880776 @default.
• W2030835318 cites W2013545053 @default.
• W2030835318 cites W2022636431 @default.
• W2030835318 cites W2031878808 @default.
• W2030835318 cites W2037653294 @default.
• W2030835318 cites W2053033498 @default.
• W2030835318 cites W2054342062 @default.
• W2030835318 cites W2057553106 @default.
• W2030835318 cites W2057991576 @default.
• W2030835318 cites W2062948593 @default.
• W2030835318 cites W2074351361 @default.
• W2030835318 cites W2076589464 @default.
• W2030835318 cites W2091520144 @default.
• W2030835318 cites W2108388593 @default.
• W2030835318 cites W307794556 @default.
• W2030835318 cites W349121293 @default.
• W2030835318 cites W4233090981 @default.
• W2030835318 doi "https://doi.org/10.1074/jbc.272.40.25373" @default.
• W2030835318 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9312158" @default.
• W2030835318 hasPublicationYear "1997" @default.
• W2030835318 type Work @default.
• W2030835318 sameAs 2030835318 @default.
• W2030835318 citedByCount "20" @default.
• W2030835318 countsByYear W20308353182014 @default.
• W2030835318 countsByYear W20308353182015 @default.
• W2030835318 countsByYear W20308353182016 @default.
• W2030835318 crossrefType "journal-article" @default.
• W2030835318 hasAuthorship W2030835318A5000404270 @default.
• W2030835318 hasAuthorship W2030835318A5032651935 @default.
• W2030835318 hasBestOaLocation W20308353181 @default.
• W2030835318 hasConcept C104317684 @default.
• W2030835318 hasConcept C138885662 @default.
• W2030835318 hasConcept C170493617 @default.
• W2030835318 hasConcept C179926584 @default.
• W2030835318 hasConcept C41895202 @default.
• W2030835318 hasConcept C54355233 @default.
• W2030835318 hasConcept C61174792 @default.
• W2030835318 hasConcept C86803240 @default.
• W2030835318 hasConceptScore W2030835318C104317684 @default.
• W2030835318 hasConceptScore W2030835318C138885662 @default.
• W2030835318 hasConceptScore W2030835318C170493617 @default.
• W2030835318 hasConceptScore W2030835318C179926584 @default.
• W2030835318 hasConceptScore W2030835318C41895202 @default.
• W2030835318 hasConceptScore W2030835318C54355233 @default.
• W2030835318 hasConceptScore W2030835318C61174792 @default.
• W2030835318 hasConceptScore W2030835318C86803240 @default.
• W2030835318 hasIssue "40" @default.
• W2030835318 hasLocation W20308353181 @default.
• W2030835318 hasOpenAccess W2030835318 @default.
• W2030835318 hasPrimaryLocation W20308353181 @default.
• W2030835318 hasRelatedWork W1818003313 @default.
• W2030835318 hasRelatedWork W1983080740 @default.
• W2030835318 hasRelatedWork W1991523530 @default.
• W2030835318 hasRelatedWork W2002128513 @default.
• W2030835318 hasRelatedWork W2020824267 @default.
• W2030835318 hasRelatedWork W2031436818 @default.
• W2030835318 hasRelatedWork W2043249063 @default.
• W2030835318 hasRelatedWork W2075354549 @default.
• W2030835318 hasRelatedWork W2171277769 @default.
• W2030835318 hasRelatedWork W2092874662 @default.
• W2030835318 hasVolume "272" @default.
• W2030835318 isParatext "false" @default.
• W2030835318 isRetracted "false" @default.
• W2030835318 magId "2030835318" @default.
• W2030835318 workType "article" @default.