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- W2030862553 abstract "Candida antarctica lipase B (CALB) has been employed as an efficient catalyst in the preparation of many flavor esters. A CALB-displaying yeast whole-cell biocatalyst could be an attractive alternative to commercial immobilized CALB because of its low-cost preparation and high enzymatic activity. We investigated the potential application of CALB-displaying Pichia pastoris cells for the production of flavor esters. The optimal conditions for flavor esters synthesis by this biocatalyst were determined in 50-ml shake flasks. Under optimized conditions, the synthesis of 12 kinds flavor esters were scaled up in a 5-l batch stirred reactor. Among these, the mole conversions of 10 exceeded 95% after reactions for 4 h. In addition, this biocatalyst showed good tolerance for high substrates concentration and excellent operational stability. Repeated use of the cells in 10 batches resulted in an activity loss of less than 10%. Thus, CALB-displaying P. pastoris whole cells are robust biocatalysts with potential commercial application in the large-scale production of flavor esters in non-aqueous media." @default.
- W2030862553 created "2016-06-24" @default.
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- W2030862553 date "2012-05-01" @default.
- W2030862553 modified "2023-10-17" @default.
- W2030862553 title "Production of flavor esters catalyzed by CALB-displaying Pichia pastoris whole-cells in a batch reactor" @default.
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- W2030862553 doi "https://doi.org/10.1016/j.jbiotec.2012.02.013" @default.
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