FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2030872703> ?p ?o ?g. }

• W2030872703 endingPage "23764" @default.
• W2030872703 startingPage "23751" @default.
• W2030872703 abstract "In pathogenic trypanosomes, trypanothione synthetase (TryS) catalyzes the synthesis of both glutathionylspermidine (Gsp) and trypanothione (bis(glutathionyl)spermidine (T(SH)2)). Here we present a thorough kinetic analysis of Trypanosoma brucei TryS in a newly developed phosphate buffer system at pH 7.0 and 37 °C, mimicking the physiological environment of the enzyme in the cytosol of bloodstream parasites. Under these conditions, TryS displays Km values for GSH, ATP, spermidine, and Gsp of 34, 18, 687, and 32 μm, respectively, as well as Ki values for GSH and T(SH)2 of 1 mm and 360 μm, respectively. As Gsp hydrolysis has a Km value of 5.6 mm, the in vivo amidase activity is probably negligible. To obtain deeper insight in the molecular mechanism of TryS, we have formulated alternative kinetic models, with elementary reaction steps represented by linear kinetic equations. The model parameters were fitted to the extensive matrix of steady-state data obtained for different substrate/product combinations under the in vivo-like conditions. The best model describes the full kinetic profile and is able to predict time course data that were not used for fitting. This system's biology approach to enzyme kinetics led us to conclude that (i) TryS follows a ter-reactant mechanism, (ii) the intermediate Gsp dissociates from the enzyme between the two catalytic steps, and (iii) T(SH)2 inhibits the enzyme by remaining bound at its product site and, as does the inhibitory GSH, by binding to the activated enzyme complex. The newly detected concerted substrate and product inhibition suggests that TryS activity is tightly regulated." @default.
• W2030872703 created "2016-06-24" @default.
• W2030872703 creator A5018863308 @default.
• W2030872703 creator A5053477937 @default.
• W2030872703 creator A5064034989 @default.
• W2030872703 creator A5069788642 @default.
• W2030872703 date "2013-08-01" @default.
• W2030872703 modified "2023-10-16" @default.
• W2030872703 title "Dissecting the Catalytic Mechanism of Trypanosoma brucei Trypanothione Synthetase by Kinetic Analysis and Computational Modeling" @default.
• W2030872703 cites W122943423 @default.
• W2030872703 cites W1426826457 @default.
• W2030872703 cites W1573720837 @default.
• W2030872703 cites W1575666547 @default.
• W2030872703 cites W1596448355 @default.
• W2030872703 cites W1669167952 @default.
• W2030872703 cites W1752036286 @default.
• W2030872703 cites W1826247155 @default.
• W2030872703 cites W1867253423 @default.
• W2030872703 cites W1963700063 @default.
• W2030872703 cites W1966445899 @default.
• W2030872703 cites W1971406466 @default.
• W2030872703 cites W1972168568 @default.
• W2030872703 cites W1981055894 @default.
• W2030872703 cites W1983909677 @default.
• W2030872703 cites W1986192026 @default.
• W2030872703 cites W1998453609 @default.
• W2030872703 cites W2004392403 @default.
• W2030872703 cites W2005647067 @default.
• W2030872703 cites W2006926156 @default.
• W2030872703 cites W2010917504 @default.
• W2030872703 cites W2016359033 @default.
• W2030872703 cites W2023772678 @default.
• W2030872703 cites W2030444682 @default.
• W2030872703 cites W2035535679 @default.
• W2030872703 cites W2045915921 @default.
• W2030872703 cites W2046013583 @default.
• W2030872703 cites W2046439293 @default.
• W2030872703 cites W2053663394 @default.
• W2030872703 cites W2055200763 @default.
• W2030872703 cites W2057685234 @default.
• W2030872703 cites W2059775525 @default.
• W2030872703 cites W2062518791 @default.
• W2030872703 cites W2063735931 @default.
• W2030872703 cites W2067142804 @default.
• W2030872703 cites W2068662199 @default.
• W2030872703 cites W2075797891 @default.
• W2030872703 cites W2082704763 @default.
• W2030872703 cites W2090001625 @default.
• W2030872703 cites W2090150963 @default.
• W2030872703 cites W2092885055 @default.
• W2030872703 cites W2102653059 @default.
• W2030872703 cites W2112238233 @default.
• W2030872703 cites W2114491796 @default.
• W2030872703 cites W2118103686 @default.
• W2030872703 cites W2121891865 @default.
• W2030872703 cites W2122124081 @default.
• W2030872703 cites W2122284068 @default.
• W2030872703 cites W2127982898 @default.
• W2030872703 cites W2132906724 @default.
• W2030872703 cites W2149268709 @default.
• W2030872703 cites W2154261496 @default.
• W2030872703 cites W2170032647 @default.
• W2030872703 cites W2340682252 @default.
• W2030872703 doi "https://doi.org/10.1074/jbc.m113.483289" @default.
• W2030872703 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3745322" @default.
• W2030872703 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23814051" @default.
• W2030872703 hasPublicationYear "2013" @default.
• W2030872703 type Work @default.
• W2030872703 sameAs 2030872703 @default.
• W2030872703 citedByCount "21" @default.
• W2030872703 countsByYear W20308727032013 @default.
• W2030872703 countsByYear W20308727032014 @default.
• W2030872703 countsByYear W20308727032015 @default.
• W2030872703 countsByYear W20308727032016 @default.
• W2030872703 countsByYear W20308727032017 @default.
• W2030872703 countsByYear W20308727032018 @default.
• W2030872703 countsByYear W20308727032019 @default.
• W2030872703 countsByYear W20308727032020 @default.
• W2030872703 countsByYear W20308727032021 @default.
• W2030872703 countsByYear W20308727032022 @default.
• W2030872703 countsByYear W20308727032023 @default.
• W2030872703 crossrefType "journal-article" @default.
• W2030872703 hasAuthorship W2030872703A5018863308 @default.
• W2030872703 hasAuthorship W2030872703A5053477937 @default.
• W2030872703 hasAuthorship W2030872703A5064034989 @default.
• W2030872703 hasAuthorship W2030872703A5069788642 @default.
• W2030872703 hasBestOaLocation W20308727031 @default.
• W2030872703 hasConcept C104317684 @default.
• W2030872703 hasConcept C121332964 @default.
• W2030872703 hasConcept C148898269 @default.
• W2030872703 hasConcept C150757390 @default.
• W2030872703 hasConcept C161790260 @default.
• W2030872703 hasConcept C181199279 @default.
• W2030872703 hasConcept C185592680 @default.
• W2030872703 hasConcept C18903297 @default.
• W2030872703 hasConcept C2777289219 @default.
• W2030872703 hasConcept C2777497464 @default.
• W2030872703 hasConcept C2779502636 @default.

• next