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- W2030878566 endingPage "265102" @default.
- W2030878566 startingPage "265102" @default.
- W2030878566 abstract "We study the spectral characteristics of bovine serum albumin (BSA) protein conjugated single-wall carbon nanotubes (SWNTs), and quantify their uptake by macrophages. The binding of BSA onto the SWNT surface is found to change the protein structure and to increase the doping of the nanotubes. The G-band Raman intensity follows a well-defined power law for SWNT concentrations of up to 33 microg ml(-1) in aqueous solutions. Subsequently, in vitro experiments demonstrate that incubation of BSA-SWNT complexes with macrophages affects neither the cellular growth nor the cellular viability over multiple cell generations. Using wide spot Raman spectroscopy as a fast, non-destructive method for statistical quantification, we observe that macrophages effectively uptake BSA-SWNT complexes, with the average number of nanotubes internalized per cell remaining relatively constant over consecutive cell generations. The number of internalized SWNTs is found to be approximately 30 10(6) SWNTs/cell for a 60 mm(-2) seeding density and approximately 100 x 10(6) SWNTs/cell for a 200 mm(-2) seeding density. Our results show that BSA-functionalized SWNTs are an efficient molecular transport system with low cytotoxicity maintained over multiple cell generations." @default.
- W2030878566 created "2016-06-24" @default.
- W2030878566 creator A5055430312 @default.
- W2030878566 creator A5056496633 @default.
- W2030878566 creator A5081285592 @default.
- W2030878566 creator A5086538449 @default.
- W2030878566 date "2013-06-04" @default.
- W2030878566 modified "2023-09-25" @default.
- W2030878566 title "Spectroscopic characterization of protein-wrapped single-wall carbon nanotubes and quantification of their cellular uptake in multiple cell generations" @default.
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- W2030878566 doi "https://doi.org/10.1088/0957-4484/24/26/265102" @default.
- W2030878566 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23735781" @default.
- W2030878566 hasPublicationYear "2013" @default.
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