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- W2030907545 abstract "All 64 possible thermodynamic parameters for a single-bulge loop in the middle of a sequence were derived from optical melting studies. The relative stability of a single bulge depended on both the type of bulged base and its flanking base pairs. The contribution of the single bulge to helix stability ranged from 3.69 kcal/mol for a TAT bulge to −1.05 kcal/mol for an ACC bulge. Thermodynamics for 10 sequences with a GTG bulge were determined to test the applicability of the nearest-neighbor model to a single-bulge loop. Thermodynamic parameters for the GTG bulge and Watson−Crick base pairs predict , ΔH°, ΔS°, and TM(50 μM) values with average deviations of 3.0%, 4.3%, 4.7%, and 0.9 °C, respectively. The prediction accuracy was within the limits of what can be expected for a nearest-neighbor model. This certified that the thermodynamics for single-bulge loops can be estimated adequately using a nearest-neighbor model." @default.
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- W2030907545 date "2004-05-07" @default.
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- W2030907545 title "Thermodynamic Parameters Based on a Nearest-Neighbor Model for DNA Sequences with a Single-Bulge Loop" @default.
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- W2030907545 doi "https://doi.org/10.1021/bi036188r" @default.
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