Matches in SemOpenAlex for { <https://semopenalex.org/work/W2030907570> ?p ?o ?g. }
- W2030907570 endingPage "1741" @default.
- W2030907570 startingPage "1738" @default.
- W2030907570 abstract "Modified capillary zone electrophoresis of zeins in strongly acidic, isoelectric buffers is reported for screening of maize (Zea mays L.) lines. The optimized background electrolyte contained 40 mM aspartic acid (pH = pI = 2.77), 8 M urea and 0.5% short-chain hydroxyethylcellulose (Mn 27000 Da) (apparent pH in 8 M urea: 3.9). Due to the low conductivity of such a buffer (0.7 mmhos), separations can be carried out at 800 V/cm, even in relatively large bore capillaries (50 microm ID), ensuring good sensitivity. The zein patterns thus obtained are species-specific and allow easy identification of all maize lines tested. No adsorption of proteins to the silica wall is observed and high reproducibility in peak areas and transit times is obtained. The relatively slow migration time (ca. 30 min), even under such a high voltage gradient, is attributed to the paucity of basic amino acid residues typical of zeins, leading to an unfavorable charge/ mass ratio due to the modest net positive charge at the operative pH." @default.
- W2030907570 created "2016-06-24" @default.
- W2030907570 creator A5035257172 @default.
- W2030907570 creator A5049258485 @default.
- W2030907570 creator A5060277927 @default.
- W2030907570 date "1998-07-01" @default.
- W2030907570 modified "2023-09-23" @default.
- W2030907570 title "Identification of maize linesvia capillary electrophoresis of zeins in isoelectric, acidic buffers" @default.
- W2030907570 cites W1565278250 @default.
- W2030907570 cites W1885787573 @default.
- W2030907570 cites W1915466838 @default.
- W2030907570 cites W1976582011 @default.
- W2030907570 cites W1995591012 @default.
- W2030907570 cites W2020523461 @default.
- W2030907570 cites W2041549174 @default.
- W2030907570 cites W2060382937 @default.
- W2030907570 cites W2074474136 @default.
- W2030907570 cites W2076927196 @default.
- W2030907570 cites W2081110052 @default.
- W2030907570 cites W2085469845 @default.
- W2030907570 cites W2089916237 @default.
- W2030907570 cites W2090527886 @default.
- W2030907570 cites W2091856362 @default.
- W2030907570 cites W2101962523 @default.
- W2030907570 cites W2102131116 @default.
- W2030907570 cites W2107359502 @default.
- W2030907570 cites W2149335063 @default.
- W2030907570 cites W317724725 @default.
- W2030907570 doi "https://doi.org/10.1002/elps.1150191035" @default.
- W2030907570 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9719554" @default.
- W2030907570 hasPublicationYear "1998" @default.
- W2030907570 type Work @default.
- W2030907570 sameAs 2030907570 @default.
- W2030907570 citedByCount "33" @default.
- W2030907570 countsByYear W20309075702012 @default.
- W2030907570 countsByYear W20309075702014 @default.
- W2030907570 countsByYear W20309075702022 @default.
- W2030907570 crossrefType "journal-article" @default.
- W2030907570 hasAuthorship W2030907570A5035257172 @default.
- W2030907570 hasAuthorship W2030907570A5049258485 @default.
- W2030907570 hasAuthorship W2030907570A5060277927 @default.
- W2030907570 hasConcept C113196181 @default.
- W2030907570 hasConcept C147789679 @default.
- W2030907570 hasConcept C150394285 @default.
- W2030907570 hasConcept C159985019 @default.
- W2030907570 hasConcept C17525397 @default.
- W2030907570 hasConcept C178790620 @default.
- W2030907570 hasConcept C181199279 @default.
- W2030907570 hasConcept C185592680 @default.
- W2030907570 hasConcept C192562407 @default.
- W2030907570 hasConcept C196806460 @default.
- W2030907570 hasConcept C2780365088 @default.
- W2030907570 hasConcept C2900643 @default.
- W2030907570 hasConcept C2993531722 @default.
- W2030907570 hasConcept C40250595 @default.
- W2030907570 hasConcept C43617362 @default.
- W2030907570 hasConcept C50476208 @default.
- W2030907570 hasConcept C55493867 @default.
- W2030907570 hasConcept C6557445 @default.
- W2030907570 hasConcept C68801617 @default.
- W2030907570 hasConcept C86803240 @default.
- W2030907570 hasConcept C96294017 @default.
- W2030907570 hasConceptScore W2030907570C113196181 @default.
- W2030907570 hasConceptScore W2030907570C147789679 @default.
- W2030907570 hasConceptScore W2030907570C150394285 @default.
- W2030907570 hasConceptScore W2030907570C159985019 @default.
- W2030907570 hasConceptScore W2030907570C17525397 @default.
- W2030907570 hasConceptScore W2030907570C178790620 @default.
- W2030907570 hasConceptScore W2030907570C181199279 @default.
- W2030907570 hasConceptScore W2030907570C185592680 @default.
- W2030907570 hasConceptScore W2030907570C192562407 @default.
- W2030907570 hasConceptScore W2030907570C196806460 @default.
- W2030907570 hasConceptScore W2030907570C2780365088 @default.
- W2030907570 hasConceptScore W2030907570C2900643 @default.
- W2030907570 hasConceptScore W2030907570C2993531722 @default.
- W2030907570 hasConceptScore W2030907570C40250595 @default.
- W2030907570 hasConceptScore W2030907570C43617362 @default.
- W2030907570 hasConceptScore W2030907570C50476208 @default.
- W2030907570 hasConceptScore W2030907570C55493867 @default.
- W2030907570 hasConceptScore W2030907570C6557445 @default.
- W2030907570 hasConceptScore W2030907570C68801617 @default.
- W2030907570 hasConceptScore W2030907570C86803240 @default.
- W2030907570 hasConceptScore W2030907570C96294017 @default.
- W2030907570 hasIssue "10" @default.
- W2030907570 hasLocation W20309075701 @default.
- W2030907570 hasLocation W20309075702 @default.
- W2030907570 hasOpenAccess W2030907570 @default.
- W2030907570 hasPrimaryLocation W20309075701 @default.
- W2030907570 hasRelatedWork W109922730 @default.
- W2030907570 hasRelatedWork W115908265 @default.
- W2030907570 hasRelatedWork W1967452203 @default.
- W2030907570 hasRelatedWork W1970374638 @default.
- W2030907570 hasRelatedWork W1982499865 @default.
- W2030907570 hasRelatedWork W2061243012 @default.
- W2030907570 hasRelatedWork W2077855486 @default.
- W2030907570 hasRelatedWork W2081064559 @default.
- W2030907570 hasRelatedWork W2131261912 @default.
- W2030907570 hasRelatedWork W2799656108 @default.