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• W2030947247 abstract "This study reports the preparation and characterization of cysteine-terminated B-domain (Bd-cys) of Staphylococcus aureus protein A, in combination with immunoglobulin G (IgG) antibodies directed against the ρ1 and α1 subunits of GABAA receptors, for localizing reagents of interest to the target receptor. A cysteine residue was inserted at the C terminus of the cysteine-lacking B-domain (Bd) and used for conjugating maleimide-containing compounds. As determined by enzyme-linked immunosorbent assay (ELISA), binding of a Bd-cys-S-fluorescein conjugate to polyclonal guinea pig anti-GABAA-ρ1 and rabbit anti-GABAA-α1 IgG was similar to that exhibited by full-length protein A. Surface plasmon resonance analysis of the interaction of Bd-cys-S-PEG3400-biotin conjugate (where PEG is polyethylene glycol) with anti-GABAA-ρ1 and anti-GABAA-α1 yielded KD values of 6.4 ± 1.9 and 0.4 ± 0.1 nM, respectively. Fluorescence anisotropy analysis of the binding of Bd-cys-S-fluorescein to the two antibodies yielded EC50 values of 65 and 18 nM, respectively. As determined with biotin-reactive fluorescent reagents, Bd-cys-S-PEG3400-biotin specifically bound to the plasma membrane of Xenopus laevis oocytes that expressed α1β2γ2 or homomeric ρ1 GABAA receptors and were pretreated with the corresponding anti-GABAA IgG. The IgG-binding specificity and high affinity of Bd-cys conjugates illustrate the potential of these conjugates, in combination with a selected IgG, to localize compounds of interest at specific cell surface proteins." @default.
• W2030947247 created "2016-06-24" @default.
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• W2030947247 date "2013-01-01" @default.
• W2030947247 modified "2023-10-16" @default.
• W2030947247 title "Cysteine-terminated B-domain of Staphylococcus aureus protein A as a scaffold for targeting GABAA receptors" @default.
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• W2030947247 doi "https://doi.org/10.1016/j.ab.2012.08.031" @default.
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