Matches in SemOpenAlex for { <https://semopenalex.org/work/W2030950187> ?p ?o ?g. }
Showing items 1 to 88 of
88
with 100 items per page.
- W2030950187 endingPage "760" @default.
- W2030950187 startingPage "752" @default.
- W2030950187 abstract "S-adenosylmethionine (SAM), the most important methyl donor in human body, is generally used to treat cholestasis in clinic. In recent years, SAM has been found to have inhibitory effects on breast cancer, liver cancer and colon carcinoma. This study was to investigate the inhibitory effects of SAM on human gastric cancer cells in vivo and in vitro, and the antitumor mechanisms.The effects of SAM on the proliferation of gastric cancer SGC-7901 and MKN-45 cells were determined by MTT assay. After SGC-7901 and MKN-45 cells were treated with 0, 2, and 4 mmol/L SAM for 72 h, the expression and methylation of c-myc and urokinase type plasminogen activator (uPA) were detected by reverse transcription-polymerase chain reaction (RT-PCR) and methylation-specific PCR (MSP). Tumor xenografts were established by injecting SGC-7901 cells subcutaneously in BALB/c nude mice. The mice were randomized into low concentration group [192 µmol/(kg · day)], high concentration group [768 µmol/(kg · day)], and control group [normal saline (NS)], and received peritoneal injection of relative reagents for 15 days. The tumor size was measured, the protein and mRNA expression of c-myc and uPA were detected by immunohistochemistry and RT-PCR, and the methylation of c-myc and uPA genes was detected by MSP.SAM inhibited the growth of SGC-7901 and MKN-45 cells obviously and the effects were enhanced with the increase of SAM concentration and treatment time. The mRNA expression of c-myc and uPA in SGC-7901 cells and that of uPA in MKN-45 cells significantly decreased. The c-myc and uPA genes in SGC-7901 cells and uPA gene in MKN-45 cells were partly or completely methylated after SAM treatment. The tumor volume was significantly lower in low concentration group [(618.51 ± 149.27) mm³] and high concentration group [(444.32 ± 118.51) mm³] than in control group [(1018.22 ± 223.07) mm³] (both P < 0.01). The inhibitory rates of tumor growth were 39.26% in low concentration group and 56.36% in high concentration group. The protein and mRNA expressions of c-myc and uPA were remarkably reduced (all P < 0.01), and the hypomethylation of c-myc and uPA genes were reversed after SAM treatment.SAM can inhibit the growth of human gastric cancer cells both in vivo and in vitro. The mechanism may be that SAM can reverse the hypomethylation of c-myc and uPA genes, reduce their expression, and then inhibit tumor growth." @default.
- W2030950187 created "2016-06-24" @default.
- W2030950187 creator A5010777233 @default.
- W2030950187 creator A5015741830 @default.
- W2030950187 creator A5036913519 @default.
- W2030950187 creator A5062203269 @default.
- W2030950187 creator A5083607904 @default.
- W2030950187 date "2010-08-05" @default.
- W2030950187 modified "2023-10-17" @default.
- W2030950187 title "Inhibitory effect of S-adenosylmethionine on the growth of human gastric cancer cells in vivo and in vitro" @default.
- W2030950187 doi "https://doi.org/10.5732/cjc.010.10046" @default.
- W2030950187 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20663323" @default.
- W2030950187 hasPublicationYear "2010" @default.
- W2030950187 type Work @default.
- W2030950187 sameAs 2030950187 @default.
- W2030950187 citedByCount "27" @default.
- W2030950187 countsByYear W20309501872013 @default.
- W2030950187 countsByYear W20309501872014 @default.
- W2030950187 countsByYear W20309501872015 @default.
- W2030950187 countsByYear W20309501872017 @default.
- W2030950187 countsByYear W20309501872018 @default.
- W2030950187 countsByYear W20309501872019 @default.
- W2030950187 countsByYear W20309501872020 @default.
- W2030950187 countsByYear W20309501872022 @default.
- W2030950187 countsByYear W20309501872023 @default.
- W2030950187 crossrefType "journal-article" @default.
- W2030950187 hasAuthorship W2030950187A5010777233 @default.
- W2030950187 hasAuthorship W2030950187A5015741830 @default.
- W2030950187 hasAuthorship W2030950187A5036913519 @default.
- W2030950187 hasAuthorship W2030950187A5062203269 @default.
- W2030950187 hasAuthorship W2030950187A5083607904 @default.
- W2030950187 hasBestOaLocation W20309501871 @default.
- W2030950187 hasConcept C104317684 @default.
- W2030950187 hasConcept C121608353 @default.
- W2030950187 hasConcept C126322002 @default.
- W2030950187 hasConcept C134018914 @default.
- W2030950187 hasConcept C150903083 @default.
- W2030950187 hasConcept C153911025 @default.
- W2030950187 hasConcept C185592680 @default.
- W2030950187 hasConcept C202751555 @default.
- W2030950187 hasConcept C207001950 @default.
- W2030950187 hasConcept C2779679481 @default.
- W2030950187 hasConcept C33288867 @default.
- W2030950187 hasConcept C502942594 @default.
- W2030950187 hasConcept C55493867 @default.
- W2030950187 hasConcept C56219504 @default.
- W2030950187 hasConcept C71924100 @default.
- W2030950187 hasConcept C86803240 @default.
- W2030950187 hasConcept C96232424 @default.
- W2030950187 hasConceptScore W2030950187C104317684 @default.
- W2030950187 hasConceptScore W2030950187C121608353 @default.
- W2030950187 hasConceptScore W2030950187C126322002 @default.
- W2030950187 hasConceptScore W2030950187C134018914 @default.
- W2030950187 hasConceptScore W2030950187C150903083 @default.
- W2030950187 hasConceptScore W2030950187C153911025 @default.
- W2030950187 hasConceptScore W2030950187C185592680 @default.
- W2030950187 hasConceptScore W2030950187C202751555 @default.
- W2030950187 hasConceptScore W2030950187C207001950 @default.
- W2030950187 hasConceptScore W2030950187C2779679481 @default.
- W2030950187 hasConceptScore W2030950187C33288867 @default.
- W2030950187 hasConceptScore W2030950187C502942594 @default.
- W2030950187 hasConceptScore W2030950187C55493867 @default.
- W2030950187 hasConceptScore W2030950187C56219504 @default.
- W2030950187 hasConceptScore W2030950187C71924100 @default.
- W2030950187 hasConceptScore W2030950187C86803240 @default.
- W2030950187 hasConceptScore W2030950187C96232424 @default.
- W2030950187 hasIssue "8" @default.
- W2030950187 hasLocation W20309501871 @default.
- W2030950187 hasLocation W20309501872 @default.
- W2030950187 hasOpenAccess W2030950187 @default.
- W2030950187 hasPrimaryLocation W20309501871 @default.
- W2030950187 hasRelatedWork W1576290062 @default.
- W2030950187 hasRelatedWork W1998257795 @default.
- W2030950187 hasRelatedWork W2007364335 @default.
- W2030950187 hasRelatedWork W2058719591 @default.
- W2030950187 hasRelatedWork W2085429393 @default.
- W2030950187 hasRelatedWork W2152119619 @default.
- W2030950187 hasRelatedWork W2259686789 @default.
- W2030950187 hasRelatedWork W2333808022 @default.
- W2030950187 hasRelatedWork W2386538333 @default.
- W2030950187 hasRelatedWork W4220828450 @default.
- W2030950187 hasVolume "29" @default.
- W2030950187 isParatext "false" @default.
- W2030950187 isRetracted "false" @default.
- W2030950187 magId "2030950187" @default.
- W2030950187 workType "article" @default.