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- W2031073330 abstract "We employ a combination of (13)C/(15)N magic angle spinning (MAS) NMR and (2)H NMR to study the structural and functional consequences of different membrane environments on VDAC1 and, conversely, the effect of VDAC1 on the structure of the lipid bilayer. MAS spectra reveal a well-structured VDAC1 in 2D crystals of dimyristoylphosphatidylcholine (DMPC) and diphytanoylphosphatidylcholine (DPhPC), and their temperature dependence suggests that the VDAC structure does not change conformation above and below the lipid phase transition temperature. The same data show that the N-terminus remains structured at both low and high temperatures. Importantly, functional studies based on electrophysiological measurements on these same samples show fully functional channels, even without the presence of Triton X-100 that has been found necessary for in vitro-refolded channels. (2)H solid-state NMR and differential scanning calorimetry were used to investigate the dynamics and phase behavior of the lipids within the VDAC1 2D crystals. (2)H NMR spectra indicate that the presence of protein in DMPC results in a broad lipid phase transition that is shifted from 19 to ~27 °C and show the existence of different lipid populations, consistent with the presence of both annular and bulk lipids in the functionally and structurally homogeneous samples." @default.
- W2031073330 created "2016-06-24" @default.
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- W2031073330 date "2012-03-30" @default.
- W2031073330 modified "2023-10-02" @default.
- W2031073330 title "Lipid Dynamics and Protein–Lipid Interactions in 2D Crystals Formed with the β-Barrel Integral Membrane Protein VDAC1" @default.
- W2031073330 cites W1486363145 @default.
- W2031073330 cites W1510785746 @default.
- W2031073330 cites W160998324 @default.
- W2031073330 cites W1902502177 @default.
- W2031073330 cites W1964472954 @default.
- W2031073330 cites W1964477055 @default.
- W2031073330 cites W1967068772 @default.
- W2031073330 cites W1968779811 @default.
- W2031073330 cites W1969642737 @default.
- W2031073330 cites W1975301251 @default.
- W2031073330 cites W1978974783 @default.
- W2031073330 cites W1980478162 @default.
- W2031073330 cites W1980529669 @default.
- W2031073330 cites W1984076817 @default.
- W2031073330 cites W1984180966 @default.
- W2031073330 cites W1986199954 @default.
- W2031073330 cites W1991655565 @default.
- W2031073330 cites W1993801027 @default.
- W2031073330 cites W1994001831 @default.
- W2031073330 cites W2003960181 @default.
- W2031073330 cites W2004387249 @default.
- W2031073330 cites W2004721322 @default.
- W2031073330 cites W2007552499 @default.
- W2031073330 cites W2009385094 @default.
- W2031073330 cites W2009452143 @default.
- W2031073330 cites W2010150797 @default.
- W2031073330 cites W2011698799 @default.
- W2031073330 cites W2012093444 @default.
- W2031073330 cites W2014784084 @default.
- W2031073330 cites W2015144711 @default.
- W2031073330 cites W2022101585 @default.
- W2031073330 cites W2022365712 @default.
- W2031073330 cites W2023388639 @default.
- W2031073330 cites W2025557161 @default.
- W2031073330 cites W2030349442 @default.
- W2031073330 cites W2035458328 @default.
- W2031073330 cites W2035500993 @default.
- W2031073330 cites W2036679207 @default.
- W2031073330 cites W2037397796 @default.
- W2031073330 cites W2038054912 @default.
- W2031073330 cites W2041926994 @default.
- W2031073330 cites W2042249221 @default.
- W2031073330 cites W2042539388 @default.
- W2031073330 cites W2045649071 @default.
- W2031073330 cites W2046427301 @default.
- W2031073330 cites W2049399715 @default.
- W2031073330 cites W2049802226 @default.
- W2031073330 cites W2050292589 @default.
- W2031073330 cites W2051742147 @default.
- W2031073330 cites W2055134616 @default.
- W2031073330 cites W2056527675 @default.
- W2031073330 cites W2057837015 @default.
- W2031073330 cites W2059166011 @default.
- W2031073330 cites W2059323582 @default.
- W2031073330 cites W2059823462 @default.
- W2031073330 cites W2062575082 @default.
- W2031073330 cites W2064023056 @default.
- W2031073330 cites W2064225366 @default.
- W2031073330 cites W2066328997 @default.
- W2031073330 cites W2066879788 @default.
- W2031073330 cites W2067204369 @default.
- W2031073330 cites W2067691043 @default.
- W2031073330 cites W2067715255 @default.
- W2031073330 cites W2070504376 @default.
- W2031073330 cites W2072234331 @default.
- W2031073330 cites W2072683739 @default.
- W2031073330 cites W2074370519 @default.
- W2031073330 cites W2076228202 @default.
- W2031073330 cites W2076243860 @default.
- W2031073330 cites W2077327464 @default.
- W2031073330 cites W2078915131 @default.
- W2031073330 cites W2081833666 @default.
- W2031073330 cites W2083039757 @default.
- W2031073330 cites W2084868629 @default.
- W2031073330 cites W2086932069 @default.
- W2031073330 cites W2088344339 @default.
- W2031073330 cites W2090280755 @default.
- W2031073330 cites W2091961600 @default.
- W2031073330 cites W2097347505 @default.
- W2031073330 cites W2106912222 @default.
- W2031073330 cites W2107664487 @default.
- W2031073330 cites W2108364101 @default.
- W2031073330 cites W2111836142 @default.
- W2031073330 cites W2114526613 @default.