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- W2031085890 abstract "RATIONALE: CRTh2 is a receptor for PGD2, a major mediator released by mast cells following degranulation and a marker of human Th2 cells. PGD2-CRTh2 signaling induces expression of IL-4, IL-13 and IL-5. CRTh2 is upregulated by IL-4 and GATA-3, however, further study is required to characterize the regulatory elements mediating CRTh2 transcription. METHODS: Luciferase promoter constructs containing a 450 base pair fragment of the CRTh2 promoter (CRTh2pro-Luc) and two conserved region (CR) from the intron (CRTh2pro-Luc-CR1 and CRTh2pro-Luc-CR2) were generated. Activity of these constructs was studied by transient transfection of Jurkat T cells following overnight stimulation with PMA and ionomycin (P/I, 20ng/ml and 1μM). RESULTS: CRTh2pro-Luc activity was induced by P/I. CRTh2pro-Luc-CR1 activity was higher than CRTh2pro-Luc, while CRTh2pro-Luc-CR2 was actually less active. In silico analysis revealed the presence of putative GATA, NFAT and STAT sites within the promoter. Over-expression of GATA-3 further increased P/I induction of CRTh2 promoter activity. CR1 contains 9 potential GATA motifs while CR2 contains 4 putative STAT sites, the major transcription factor downstream of IL-4. Over-expression of GATA-3 with CRTh2pro-Luc-CR1 was more active than CRTh2pro-Luc, while STAT6 did not enhance CRTh2pro-Luc-CR2 or CRTh2-pro-Luc. CONCLUSIONS: GATA-3, a Th2 differentiation factor, induces CRTh2 transcription. Although CR2 did not demonstrate transcriptional activity, even with STAT6 over-expression, this region may still be important for chromatin remodeling." @default.
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- W2031085890 date "2007-01-01" @default.
- W2031085890 modified "2023-09-27" @default.
- W2031085890 title "GATA-3 Induces Transcriptional Activation of CRTh2" @default.
- W2031085890 doi "https://doi.org/10.1016/j.jaci.2006.12.051" @default.
- W2031085890 hasPublicationYear "2007" @default.
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