Matches in SemOpenAlex for { <https://semopenalex.org/work/W2031102121> ?p ?o ?g. }
- W2031102121 endingPage "1303" @default.
- W2031102121 startingPage "1297" @default.
- W2031102121 abstract "Tobacco smoking is one of the leading causes for oral cancer. Dibenzo[a,l]pyrene (DB[a,l]P), an environmental pollutant and a tobacco smoke constituent, is the most carcinogenic polycyclic aromatic hydrocarbon (PAH) tested to date in several animal models (target organs: skin, lung, ovary, and mammary tissues). We have recently demonstrated that DB[a,l]P is also capable of inducing oral cancer in mice; however, its metabolic activation to the ultimate genotoxic metabolite dibenzo[a,l]pyrene-11,12-dihydrodiol-13,14-epoxide (DB[a,l]PDE) in mouse oral cavity has not been examined. Here we developed a liquid chromatography–tandem mass spectrometry (LC-MS/MS) method to detect and quantify (±)-anti-DB[a,l]PDE-dA adducts in oral tissues of mice treated with DB[a,l]P. [15N5]-(±)-anti-DB[a,l]PDE-N6-dA adducts were synthesized as internal standards. The stereoisomeric adducts were characterized by MS, NMR, and CD analysis. The detection limit of the method is 8 fmol with 100 μg of digested DNA as the matrix. Two adducts were detected and identified as (−)-anti-cis and (−)-anti-trans-DB[a,l]PDE-dA in the oral tissues of mice following the direct application of DB[a,l]P (240 nmol per day, for 2 days) into the oral cavity, indicating that DB[a,l]P is predominantly metabolized into (−)-anti-DB[a,l]PDE in this target organ. We also compared the formation and removal of adducts as a function of time, following the direct application of DB[a,l]P (24 nmol, 3 times per week for 5 weeks) into the oral cavity of mice. Adducts were quantified at 48 h, 1, 2, and 4 weeks after the last dose. Maximal levels of adducts occurred at 48 h, followed by a gradual decrease. The levels (fmol/μg DNA) of (−)-anti-trans adducts (4.03 ± 0.27 to 1.77 ± 0.25) are significantly higher than (−)-anti-cis-DB[a,l]PDE-dA adduct (1.63 ± 0.42 to 0.72 ± 0.04) at each time point (p < 0.005). The results presented here indicate that the formation and persistence of (−)-anti-DB[a,l]PDE-dA adducts may, in part, contribute to the initiation of DB[a,l]P-induced oral carcinogenesis." @default.
- W2031102121 created "2016-06-24" @default.
- W2031102121 creator A5001311093 @default.
- W2031102121 creator A5018954418 @default.
- W2031102121 creator A5048058203 @default.
- W2031102121 creator A5072656505 @default.
- W2031102121 creator A5075421344 @default.
- W2031102121 creator A5080527856 @default.
- W2031102121 creator A5083262817 @default.
- W2031102121 creator A5091385286 @default.
- W2031102121 date "2011-07-19" @default.
- W2031102121 modified "2023-10-16" @default.
- W2031102121 title "Identification and Quantification of DNA Adducts in the Oral Tissues of Mice Treated with the Environmental Carcinogen Dibenzo[<i>a,l</i>]pyrene by HPLC-MS/MS" @default.
- W2031102121 cites W1967602990 @default.
- W2031102121 cites W1972650069 @default.
- W2031102121 cites W1973125771 @default.
- W2031102121 cites W1993369268 @default.
- W2031102121 cites W1994824322 @default.
- W2031102121 cites W1997828131 @default.
- W2031102121 cites W1998218758 @default.
- W2031102121 cites W2002902222 @default.
- W2031102121 cites W2005943555 @default.
- W2031102121 cites W2010950433 @default.
- W2031102121 cites W2012588975 @default.
- W2031102121 cites W2013141013 @default.
- W2031102121 cites W2016081624 @default.
- W2031102121 cites W2024386749 @default.
- W2031102121 cites W2030797637 @default.
- W2031102121 cites W2033602942 @default.
- W2031102121 cites W2051869453 @default.
- W2031102121 cites W2054836547 @default.
- W2031102121 cites W2055084808 @default.
- W2031102121 cites W2065091408 @default.
- W2031102121 cites W2073336292 @default.
- W2031102121 cites W2081836972 @default.
- W2031102121 cites W2084178406 @default.
- W2031102121 cites W2085472892 @default.
- W2031102121 cites W2086431079 @default.
- W2031102121 cites W2094545652 @default.
- W2031102121 cites W2124062352 @default.
- W2031102121 cites W2132057054 @default.
- W2031102121 cites W2147364311 @default.
- W2031102121 cites W2148913517 @default.
- W2031102121 cites W2155073279 @default.
- W2031102121 cites W2157933551 @default.
- W2031102121 cites W2172916891 @default.
- W2031102121 cites W4240820843 @default.
- W2031102121 cites W99900191 @default.
- W2031102121 doi "https://doi.org/10.1021/tx200188j" @default.
- W2031102121 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3160270" @default.
- W2031102121 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21736370" @default.
- W2031102121 hasPublicationYear "2011" @default.
- W2031102121 type Work @default.
- W2031102121 sameAs 2031102121 @default.
- W2031102121 citedByCount "32" @default.
- W2031102121 countsByYear W20311021212012 @default.
- W2031102121 countsByYear W20311021212013 @default.
- W2031102121 countsByYear W20311021212014 @default.
- W2031102121 countsByYear W20311021212015 @default.
- W2031102121 countsByYear W20311021212016 @default.
- W2031102121 countsByYear W20311021212017 @default.
- W2031102121 countsByYear W20311021212018 @default.
- W2031102121 countsByYear W20311021212019 @default.
- W2031102121 countsByYear W20311021212021 @default.
- W2031102121 countsByYear W20311021212022 @default.
- W2031102121 crossrefType "journal-article" @default.
- W2031102121 hasAuthorship W2031102121A5001311093 @default.
- W2031102121 hasAuthorship W2031102121A5018954418 @default.
- W2031102121 hasAuthorship W2031102121A5048058203 @default.
- W2031102121 hasAuthorship W2031102121A5072656505 @default.
- W2031102121 hasAuthorship W2031102121A5075421344 @default.
- W2031102121 hasAuthorship W2031102121A5080527856 @default.
- W2031102121 hasAuthorship W2031102121A5083262817 @default.
- W2031102121 hasAuthorship W2031102121A5091385286 @default.
- W2031102121 hasBestOaLocation W20311021212 @default.
- W2031102121 hasConcept C107872376 @default.
- W2031102121 hasConcept C108204754 @default.
- W2031102121 hasConcept C114246631 @default.
- W2031102121 hasConcept C178790620 @default.
- W2031102121 hasConcept C185592680 @default.
- W2031102121 hasConcept C2777477808 @default.
- W2031102121 hasConcept C2777538855 @default.
- W2031102121 hasConcept C2778951431 @default.
- W2031102121 hasConcept C2779033964 @default.
- W2031102121 hasConcept C43617362 @default.
- W2031102121 hasConcept C55493867 @default.
- W2031102121 hasConceptScore W2031102121C107872376 @default.
- W2031102121 hasConceptScore W2031102121C108204754 @default.
- W2031102121 hasConceptScore W2031102121C114246631 @default.
- W2031102121 hasConceptScore W2031102121C178790620 @default.
- W2031102121 hasConceptScore W2031102121C185592680 @default.
- W2031102121 hasConceptScore W2031102121C2777477808 @default.
- W2031102121 hasConceptScore W2031102121C2777538855 @default.
- W2031102121 hasConceptScore W2031102121C2778951431 @default.
- W2031102121 hasConceptScore W2031102121C2779033964 @default.
- W2031102121 hasConceptScore W2031102121C43617362 @default.
- W2031102121 hasConceptScore W2031102121C55493867 @default.
- W2031102121 hasIssue "8" @default.