Matches in SemOpenAlex for { <https://semopenalex.org/work/W2031143554> ?p ?o ?g. }
Showing items 1 to 73 of
73
with 100 items per page.
- W2031143554 endingPage "8" @default.
- W2031143554 startingPage "282" @default.
- W2031143554 abstract "The automated amino acid analyzer has been used to provide a simple, rapid and accurate means of assaying the glycosaminoglycan (GAG) and collagen content, and collagen type of connective tissues. The analysis of rabbit articular cartilage using this method is reported. Cartilage slices taken from the tibial plateaus of rabbits were dried and weighed. A comparison study showed that oven-drying (110 degrees C for 24 hours) was more effective in dehydrating cartilage than vacuum dessication for 96 hours. This drying temperature could be maintained for 24 hours without destroying hexosamine. The dried samples were hydrolyzed for 4 hours in 4 N HCl at 100 degrees C; these conditions were shown to give virtually 100% release of galactosamine from purified chondroitin-6-sulphate with minimal degradation of the hexosamine. The 4-hour hydrolysates were assayed for galactosamine and glucosamine using the amino acid analyzer. From these values the amount of chondroitin sulphate and keratan sulphate were calculated. An aliquot of the 4-hour hydrolysate was hydrolyzed further in 6 N HCl for 24 hours. This destroyed hexosamine but permitted the quantitation of hydroxyproline and hydroxylysine using the amino acid analyzer. Collagen content was calculated from the hydroxyproline value and the hydroxylysine to hydroxyproline ratio was used to determine the collagen type." @default.
- W2031143554 created "2016-06-24" @default.
- W2031143554 creator A5009310950 @default.
- W2031143554 creator A5027981853 @default.
- W2031143554 creator A5078302303 @default.
- W2031143554 creator A5073697510 @default.
- W2031143554 date "1980-01-01" @default.
- W2031143554 modified "2023-09-23" @default.
- W2031143554 title "Biochemical analysis of rabbit articular cartilage using an amino acid analyzer." @default.
- W2031143554 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/6768486" @default.
- W2031143554 hasPublicationYear "1980" @default.
- W2031143554 type Work @default.
- W2031143554 sameAs 2031143554 @default.
- W2031143554 citedByCount "6" @default.
- W2031143554 crossrefType "journal-article" @default.
- W2031143554 hasAuthorship W2031143554A5009310950 @default.
- W2031143554 hasAuthorship W2031143554A5027981853 @default.
- W2031143554 hasAuthorship W2031143554A5073697510 @default.
- W2031143554 hasAuthorship W2031143554A5078302303 @default.
- W2031143554 hasConcept C105702510 @default.
- W2031143554 hasConcept C153074725 @default.
- W2031143554 hasConcept C16993767 @default.
- W2031143554 hasConcept C185592680 @default.
- W2031143554 hasConcept C2776016237 @default.
- W2031143554 hasConcept C2776661909 @default.
- W2031143554 hasConcept C2776732574 @default.
- W2031143554 hasConcept C2778125326 @default.
- W2031143554 hasConcept C2779797417 @default.
- W2031143554 hasConcept C2780550940 @default.
- W2031143554 hasConcept C2781467277 @default.
- W2031143554 hasConcept C28800991 @default.
- W2031143554 hasConcept C43617362 @default.
- W2031143554 hasConcept C515207424 @default.
- W2031143554 hasConcept C55493867 @default.
- W2031143554 hasConcept C71924100 @default.
- W2031143554 hasConcept C94412978 @default.
- W2031143554 hasConceptScore W2031143554C105702510 @default.
- W2031143554 hasConceptScore W2031143554C153074725 @default.
- W2031143554 hasConceptScore W2031143554C16993767 @default.
- W2031143554 hasConceptScore W2031143554C185592680 @default.
- W2031143554 hasConceptScore W2031143554C2776016237 @default.
- W2031143554 hasConceptScore W2031143554C2776661909 @default.
- W2031143554 hasConceptScore W2031143554C2776732574 @default.
- W2031143554 hasConceptScore W2031143554C2778125326 @default.
- W2031143554 hasConceptScore W2031143554C2779797417 @default.
- W2031143554 hasConceptScore W2031143554C2780550940 @default.
- W2031143554 hasConceptScore W2031143554C2781467277 @default.
- W2031143554 hasConceptScore W2031143554C28800991 @default.
- W2031143554 hasConceptScore W2031143554C43617362 @default.
- W2031143554 hasConceptScore W2031143554C515207424 @default.
- W2031143554 hasConceptScore W2031143554C55493867 @default.
- W2031143554 hasConceptScore W2031143554C71924100 @default.
- W2031143554 hasConceptScore W2031143554C94412978 @default.
- W2031143554 hasIssue "146" @default.
- W2031143554 hasLocation W20311435541 @default.
- W2031143554 hasOpenAccess W2031143554 @default.
- W2031143554 hasPrimaryLocation W20311435541 @default.
- W2031143554 hasRelatedWork W2001097268 @default.
- W2031143554 hasRelatedWork W2009701344 @default.
- W2031143554 hasRelatedWork W2031143554 @default.
- W2031143554 hasRelatedWork W2069012619 @default.
- W2031143554 hasRelatedWork W2077757928 @default.
- W2031143554 hasRelatedWork W2082886042 @default.
- W2031143554 hasRelatedWork W2410112713 @default.
- W2031143554 hasRelatedWork W2418057574 @default.
- W2031143554 hasRelatedWork W3082856847 @default.
- W2031143554 hasRelatedWork W3139528098 @default.
- W2031143554 isParatext "false" @default.
- W2031143554 isRetracted "false" @default.
- W2031143554 magId "2031143554" @default.
- W2031143554 workType "article" @default.