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• W2031146881 startingPage "649" @default.
• W2031146881 abstract "Abstract Simple sequence repeat ( SSR ) or microsatellite markers are a valuable tool for several purposes such as evaluation of genetic diversity, fingerprinting, marker‐assisted selection and breeding. In this study, a SSR genomic enriched library was developed in L athyrus sativus (grass pea) by affinity capture of restriction fragments to biotinylated microsatellite oligonucleotides. About 400 randomly selected clones were sequenced, and SSR s were present in approximately 30% of them. Clones contained 75%, 9% and 16% of simple, interrupted and compound SSR s, respectively. Of the 10 SSR s tested, 7 primer pairs produced clearly distinguishable DNA banding patterns. Successively, SSR primer pairs were successfully tested to reveal polymorphism in a set of four different grass pea germplasm accessions. The transferability of SSR markers was high among three related species of L athyrus , namely L athyrus cicera , L athyrus ochrus and L athyrus tingitanus , and the legume crop, P isum sativum . These results indicate that the novel SSR markers are informative and will be useful and convenient for genetic analysis in grass pea and related species." @default.
• W2031146881 created "2016-06-24" @default.
• W2031146881 creator A5007523412 @default.
• W2031146881 creator A5058658702 @default.
• W2031146881 date "2013-08-23" @default.
• W2031146881 modified "2023-10-07" @default.
• W2031146881 title "Development of genomic simple sequence repeat markers from an enriched genomic library of grass pea (<i><scp>L</scp>athyrus sativus</i> L.)" @default.
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• W2031146881 doi "https://doi.org/10.1111/pbr.12093" @default.
• W2031146881 hasPublicationYear "2013" @default.
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