Matches in SemOpenAlex for { <https://semopenalex.org/work/W2031183851> ?p ?o ?g. }
Showing items 1 to 87 of
87
with 100 items per page.
- W2031183851 endingPage "152" @default.
- W2031183851 startingPage "145" @default.
- W2031183851 abstract "We describe a series of Dictyostelium expression vectors for recombination cloning using the Gateway technology. DNA fragments generated by high fidelity polymerase chain reaction are cloned by topoisomerase-mediated ligation, then recombined into any of several Dictyostelium expression vectors using phage λ LR recombinase. No restriction enzymes are used in this procedure. Coding regions can be expressed from their own promoters, or from a strong actin 15 promoter as a native protein, or with an amino or carboxyl-terminal GFP fusion. Gene promoters of interest can be analyzed by controlled expression of GFP and β-galactosidase. These vectors allow for rapid and simple characterization of novel DNA, and are ideal for high-throughput studies." @default.
- W2031183851 created "2016-06-24" @default.
- W2031183851 creator A5023587716 @default.
- W2031183851 creator A5030304398 @default.
- W2031183851 creator A5042148058 @default.
- W2031183851 date "2006-11-01" @default.
- W2031183851 modified "2023-10-18" @default.
- W2031183851 title "A series of Dictyostelium expression vectors for recombination cloning" @default.
- W2031183851 cites W1563231805 @default.
- W2031183851 cites W1673824819 @default.
- W2031183851 cites W1879374409 @default.
- W2031183851 cites W1949079829 @default.
- W2031183851 cites W1968566426 @default.
- W2031183851 cites W1973276488 @default.
- W2031183851 cites W1981896167 @default.
- W2031183851 cites W1995610541 @default.
- W2031183851 cites W2009788953 @default.
- W2031183851 cites W2025245932 @default.
- W2031183851 cites W2032059441 @default.
- W2031183851 cites W2039493949 @default.
- W2031183851 cites W2060584742 @default.
- W2031183851 cites W2074308942 @default.
- W2031183851 cites W2092359053 @default.
- W2031183851 cites W2099479608 @default.
- W2031183851 cites W2107074049 @default.
- W2031183851 cites W2108124195 @default.
- W2031183851 cites W2165214155 @default.
- W2031183851 doi "https://doi.org/10.1016/j.plasmid.2006.04.001" @default.
- W2031183851 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16765443" @default.
- W2031183851 hasPublicationYear "2006" @default.
- W2031183851 type Work @default.
- W2031183851 sameAs 2031183851 @default.
- W2031183851 citedByCount "8" @default.
- W2031183851 countsByYear W20311838512013 @default.
- W2031183851 countsByYear W20311838512015 @default.
- W2031183851 crossrefType "journal-article" @default.
- W2031183851 hasAuthorship W2031183851A5023587716 @default.
- W2031183851 hasAuthorship W2031183851A5030304398 @default.
- W2031183851 hasAuthorship W2031183851A5042148058 @default.
- W2031183851 hasConcept C101762097 @default.
- W2031183851 hasConcept C104317684 @default.
- W2031183851 hasConcept C121050878 @default.
- W2031183851 hasConcept C150194340 @default.
- W2031183851 hasConcept C153911025 @default.
- W2031183851 hasConcept C187882448 @default.
- W2031183851 hasConcept C19924922 @default.
- W2031183851 hasConcept C199360897 @default.
- W2031183851 hasConcept C203284983 @default.
- W2031183851 hasConcept C2775889303 @default.
- W2031183851 hasConcept C41008148 @default.
- W2031183851 hasConcept C54355233 @default.
- W2031183851 hasConcept C86803240 @default.
- W2031183851 hasConceptScore W2031183851C101762097 @default.
- W2031183851 hasConceptScore W2031183851C104317684 @default.
- W2031183851 hasConceptScore W2031183851C121050878 @default.
- W2031183851 hasConceptScore W2031183851C150194340 @default.
- W2031183851 hasConceptScore W2031183851C153911025 @default.
- W2031183851 hasConceptScore W2031183851C187882448 @default.
- W2031183851 hasConceptScore W2031183851C19924922 @default.
- W2031183851 hasConceptScore W2031183851C199360897 @default.
- W2031183851 hasConceptScore W2031183851C203284983 @default.
- W2031183851 hasConceptScore W2031183851C2775889303 @default.
- W2031183851 hasConceptScore W2031183851C41008148 @default.
- W2031183851 hasConceptScore W2031183851C54355233 @default.
- W2031183851 hasConceptScore W2031183851C86803240 @default.
- W2031183851 hasIssue "3" @default.
- W2031183851 hasLocation W20311838511 @default.
- W2031183851 hasLocation W20311838512 @default.
- W2031183851 hasOpenAccess W2031183851 @default.
- W2031183851 hasPrimaryLocation W20311838511 @default.
- W2031183851 hasRelatedWork W1606260917 @default.
- W2031183851 hasRelatedWork W1977497426 @default.
- W2031183851 hasRelatedWork W2350283782 @default.
- W2031183851 hasRelatedWork W2352038341 @default.
- W2031183851 hasRelatedWork W2357354480 @default.
- W2031183851 hasRelatedWork W2381241901 @default.
- W2031183851 hasRelatedWork W2387211330 @default.
- W2031183851 hasRelatedWork W2799233078 @default.
- W2031183851 hasRelatedWork W2906217166 @default.
- W2031183851 hasRelatedWork W4315881441 @default.
- W2031183851 hasVolume "56" @default.
- W2031183851 isParatext "false" @default.
- W2031183851 isRetracted "false" @default.
- W2031183851 magId "2031183851" @default.
- W2031183851 workType "article" @default.