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• W2031194089 abstract "Abstract Multimeric uridine phosphorylase (UP) and purine nucleoside phosphorylase (PNP) of Bacillus subtilis have been expressed from genes cloned in Escherichia coli , purified, characterized, immobilized and stabilized on solid support. A new immobilization strategy has been developed for UP onto Sepabeads coated with polyethyleneamine followed by cross‐linking with aldehyde‐dextran. PNP has been immobilized onto glyoxyl‐agarose. At pH 10 and 45 °C these derivatives catalyzed the transglycosylation of 2′‐deoxyuridine to 2′‐deoxyguanosine in high yield (92%). Under the same conditions the not immobilized enzymes were promptly inactivated." @default.
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• W2031194089 date "2004-09-01" @default.
• W2031194089 modified "2023-10-15" @default.
• W2031194089 title "Synthesis of 2′-Deoxynucleosides by Transglycosylation with New Immobilized and Stabilized Uridine Phosphorylase and Purine Nucleoside Phosphorylase" @default.
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• W2031194089 doi "https://doi.org/10.1002/adsc.200404019" @default.
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