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• W2031211885 abstract "Contents The aim of this study was to examine comprehensively the effect of ascorbic acid (Asc) on frozen‐thawed canine semen. Pooled ejaculates (n = 10) were assessed for quality with a computer‐assisted sperm analyser (CASA). After centrifugation, the semen was divided into four aliquots (A–D) of which three were diluted with Uppsala 1 extender (v/v) containing different concentrations of Asc (B: 6.8 mmol/l; C: 13 mmol/l; D: 27 mmol/l). One group without Asc served as control (A). Subsequently, dilution samples were treated and cryopreserved as described previously ( Theriogenology 66 , 2006, 173 ). After thawing, samples were stored at 37°C for 1, 3 and 6 h, then examined for quality (CASA, flow cytometry, sperm chromatin structure assay; SCSA). Staining for flow cytometry was performed with FITC‐PNA and propidium iodide ( Reproduction 128 , 2004, 829 ). The SCSA was performed with both Tris‐NACL‐EDT buffer (TNE)‐ and Tris‐fructose‐citrate buffer (TFC). In the Asc‐supplemented groups, percentages of progressively motile sperm (P) were significantly lower than in the control group (A 1 h: 56.6 ± 9.8, 6 h: 18 ± 4.5; B 1 h: 51.2 ± 11.8, 6 h: 13.6 ± 5; C 1 h: 41 ± 16.2, 6 h: 11.2 ± 6.1; D 1 h: 37 ± 15.2, 6 h: 8.6 ± 5; p < 0.01), whereas, the percentages of intact cells without acrosome reactions did not differ between groups (p > 0.05). Furthermore, there were no significant differences between TNE‐ and TFC‐samples, for αT, for SD of αT or for comp αT [comp αT: TCF‐A: 2.5 ± 1.7%, TNE‐A: 3.4 ± 3.2%; TCF‐D: 2.5 ± 2%, TNE‐D: 3.3 ± 4.3%; p > 0.05]. However, samples diluted with both extenders correlated concerning αT, but not comp αT. We therefore recommend using TNE‐buffer for SCSA with cryopreserved canine semen. In addition, the average αT values did not differ significantly between the controls and all other groups (TNE‐A: 380.2 ± 89.1; TNE‐D: 338.1 ± 137.4; p > 0.05). It can be concluded that addition of Asc to cryoextender does not increase quality of frozen‐thawed canine semen." @default.
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• W2031211885 date "2009-07-01" @default.
• W2031211885 modified "2023-09-26" @default.
• W2031211885 title "Effect of Different Concentrations of Ascorbic Acid on Motility, Membrane Integrity and Chromatin Status of Frozen-Thawed Canine Spermatozoa Within Six Hours of Storage at 37°C" @default.
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• W2031211885 doi "https://doi.org/10.1111/j.1439-0531.2009.01417.x" @default.
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