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- W2031258186 abstract "Polyclonal antibodies directed against a (SerPro)10 oligopeptide and a ß-galactosidase/zygote-wall-gene fusion protein were used in in vitro translation/immunoprecipitation experiments to show that (SerPro)x is a common motif in proteins which assemble into either the zygotic or the vegetative/gametic cell walls of Chlamydomonas reinhardtii. There are at least five, and possibly as many as ten different zygote-specific proteins displaying (SerPro)x-like epitopes. Zygote-specific cDNAs corresponding to two of these proteins have been previously characterized and shown to encode several regions of (SerPro) ranging from 2 to 9 repeats. In addition, the (SerPro)10 antibody recognizes five vegetative/gamete-specific proteins from cells induced for wall synthesis. One of these five corresponds to the vegetative/gamete-wall glycoprotein GP2, as confirmed by immunoprecipitation with a polyclonal antibody to purified GP2. The GP2 antibody detects epitopes other than (SerPro)x and also immunoprecipitates a novel zygote-specific protein." @default.
- W2031258186 created "2016-06-24" @default.
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- W2031258186 date "1992-01-01" @default.
- W2031258186 modified "2023-10-14" @default.
- W2031258186 title "Zygote and vegetative cell wall proteins in Chlamydomonas reinhardtii share a common epitope, (SerPro)X" @default.
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- W2031258186 doi "https://doi.org/10.1016/0168-9452(92)90063-r" @default.
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