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- W20312866 abstract "The focus of these exercises will be to examine the various procedures of protoplast isolation. Protoplasts can be used for plant transformation, assay of gene expression, study of basic cell biology and response to stress, and a variety of other tasks. Two basic methods of protoplast isolation will be examined and applied to a variety of plant species including tobacco, rice, wheat, oak, arabidopsis, cotton, and African violet. In addition, techniques for vital staining and protoplast fusion will be examined. To facilitate quantitative studies, a method to determine the yield of a protoplast isolation will be discussed. Because the procedure of protoplast isolation is so sensitive to the particular plant and tissue type used as stock for isolation, a brief guide is provided to the development of a customized protocol." @default.
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- W20312866 date "2013-01-01" @default.
- W20312866 modified "2023-10-02" @default.
- W20312866 title "Protoplast Isolation and Fusion" @default.
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- W20312866 doi "https://doi.org/10.1016/b978-0-12-415920-4.00013-x" @default.
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