Matches in SemOpenAlex for { <https://semopenalex.org/work/W2031319246> ?p ?o ?g. }
Showing items 1 to 99 of
99
with 100 items per page.
- W2031319246 endingPage "220" @default.
- W2031319246 startingPage "209" @default.
- W2031319246 abstract "The genotoxic carcinogen aflatoxin B1 (AFB1) inhibited the calmodulin-stimulated membrane-bound (Ca2+Mg2+)-ATPase. Using the purified enzyme, 12 nmoles per ml of AFB1 caused maximum inhibition of 28% and 50%, of the acidic phospholipid-stimulated and calmodulin-activated Ca2+-ATPase activity respectively. Treatment of red cell ghosts with increasing concentrations of Triton X-100, a non-ionic detergent caused a progressive loss of both the basal and calmodulin-stimulated Ca2+-ATPase activity. The activity of the phospholipid-free, detergent-solubilized enzyme was almost fully restored by phosphatidyl serine (PS) and its sensitivity to calmodulin was restored in the presence of phosphatidyl choline (PC). Analysis of the results obtained using varying concentrations of ATP shows that AFB1 did not affect the Km and Vmax of the unstimulated enzyme whereas these parameters were reduced by about 75% and 50%, respectively, in the presence of calmodulin. Using the product of limited proteolysis by trypsin i.e. the 90 kDa fragment which still retains its calmodulin binding-domain and the 76 kDa fragment which has lost this domain, kinetic studies on the enzyme activity revealed that AFB1 inhibited the calmodulin-activated 90 kDa fragment by about 50% while the 76 kDa was not affected at all by the toxin and calmodulin. The toxin had no significant affect on the basal activity of the 90 kDa limited proteolysis fragment of the enzyme. These observations suggest that AFB1 inhibits the activated Ca2+-ATPase by binding to an important site in the calmodulin-binding domain of the enzyme. It seems likely that the toxin binds to tryptophan in the calmodulin-binding domain, thus causing a reduction in the rate at which this domain can interact with Ca2+-calmodulin or acidic phospholipids. The implication of these observations is that Ca2+-extrusion and other calmodulin-activated enzymes and processes may be slowed down during prolonged exposure to AFB1 because of its anticalmodulin effect." @default.
- W2031319246 created "2016-06-24" @default.
- W2031319246 creator A5017388231 @default.
- W2031319246 creator A5023011755 @default.
- W2031319246 creator A5037844672 @default.
- W2031319246 date "1995-08-01" @default.
- W2031319246 modified "2023-10-14" @default.
- W2031319246 title "The anticalmodulin effect of aflatoxin B1 on purified erythrocyte Ca2+-AtPase" @default.
- W2031319246 cites W1106257837 @default.
- W2031319246 cites W1512693490 @default.
- W2031319246 cites W1554726437 @default.
- W2031319246 cites W1571495929 @default.
- W2031319246 cites W1574112238 @default.
- W2031319246 cites W1590429718 @default.
- W2031319246 cites W1599011232 @default.
- W2031319246 cites W1599634353 @default.
- W2031319246 cites W1601913333 @default.
- W2031319246 cites W1605242131 @default.
- W2031319246 cites W1775749144 @default.
- W2031319246 cites W1838061312 @default.
- W2031319246 cites W1968559401 @default.
- W2031319246 cites W1977986845 @default.
- W2031319246 cites W1980492519 @default.
- W2031319246 cites W1995391619 @default.
- W2031319246 cites W2010424836 @default.
- W2031319246 cites W2010865351 @default.
- W2031319246 cites W2019581057 @default.
- W2031319246 cites W2022703044 @default.
- W2031319246 cites W2024157372 @default.
- W2031319246 cites W2026690484 @default.
- W2031319246 cites W2039554822 @default.
- W2031319246 cites W2041953490 @default.
- W2031319246 cites W2052664734 @default.
- W2031319246 cites W2063401411 @default.
- W2031319246 cites W2065078644 @default.
- W2031319246 cites W2074054469 @default.
- W2031319246 cites W2087696384 @default.
- W2031319246 cites W2100837269 @default.
- W2031319246 cites W2130174735 @default.
- W2031319246 cites W2138625446 @default.
- W2031319246 cites W2156016604 @default.
- W2031319246 cites W2210978458 @default.
- W2031319246 cites W2248451819 @default.
- W2031319246 doi "https://doi.org/10.1007/bf01540455" @default.
- W2031319246 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8562872" @default.
- W2031319246 hasPublicationYear "1995" @default.
- W2031319246 type Work @default.
- W2031319246 sameAs 2031319246 @default.
- W2031319246 citedByCount "6" @default.
- W2031319246 crossrefType "journal-article" @default.
- W2031319246 hasAuthorship W2031319246A5017388231 @default.
- W2031319246 hasAuthorship W2031319246A5023011755 @default.
- W2031319246 hasAuthorship W2031319246A5037844672 @default.
- W2031319246 hasConcept C153911025 @default.
- W2031319246 hasConcept C181199279 @default.
- W2031319246 hasConcept C185592680 @default.
- W2031319246 hasConcept C23265538 @default.
- W2031319246 hasConcept C2778918659 @default.
- W2031319246 hasConcept C2780340462 @default.
- W2031319246 hasConcept C2781307694 @default.
- W2031319246 hasConcept C28212737 @default.
- W2031319246 hasConcept C29688787 @default.
- W2031319246 hasConcept C41625074 @default.
- W2031319246 hasConcept C55493867 @default.
- W2031319246 hasConcept C86803240 @default.
- W2031319246 hasConceptScore W2031319246C153911025 @default.
- W2031319246 hasConceptScore W2031319246C181199279 @default.
- W2031319246 hasConceptScore W2031319246C185592680 @default.
- W2031319246 hasConceptScore W2031319246C23265538 @default.
- W2031319246 hasConceptScore W2031319246C2778918659 @default.
- W2031319246 hasConceptScore W2031319246C2780340462 @default.
- W2031319246 hasConceptScore W2031319246C2781307694 @default.
- W2031319246 hasConceptScore W2031319246C28212737 @default.
- W2031319246 hasConceptScore W2031319246C29688787 @default.
- W2031319246 hasConceptScore W2031319246C41625074 @default.
- W2031319246 hasConceptScore W2031319246C55493867 @default.
- W2031319246 hasConceptScore W2031319246C86803240 @default.
- W2031319246 hasIssue "4" @default.
- W2031319246 hasLocation W20313192461 @default.
- W2031319246 hasLocation W20313192462 @default.
- W2031319246 hasOpenAccess W2031319246 @default.
- W2031319246 hasPrimaryLocation W20313192461 @default.
- W2031319246 hasRelatedWork W1029593135 @default.
- W2031319246 hasRelatedWork W1975306034 @default.
- W2031319246 hasRelatedWork W1988368681 @default.
- W2031319246 hasRelatedWork W1990534696 @default.
- W2031319246 hasRelatedWork W2002189973 @default.
- W2031319246 hasRelatedWork W2045482490 @default.
- W2031319246 hasRelatedWork W2050454040 @default.
- W2031319246 hasRelatedWork W2050485954 @default.
- W2031319246 hasRelatedWork W2471989686 @default.
- W2031319246 hasRelatedWork W3010056926 @default.
- W2031319246 hasVolume "15" @default.
- W2031319246 isParatext "false" @default.
- W2031319246 isRetracted "false" @default.
- W2031319246 magId "2031319246" @default.
- W2031319246 workType "article" @default.