FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2031330996> ?p ?o ?g. }

• W2031330996 endingPage "741" @default.
• W2031330996 startingPage "711" @default.
• W2031330996 abstract "1. A mesodermal stem cell line C3H10T1/2 was induced to differentiate to muscle by adding 0.3 microM-5-aza-2'-deoxy-cytidine to the medium for 24 h. The changes in membrane currents during differentiation were studied by whole-cell recording and changes in the expression of fibronectin, Neural Cell Adhesion Molecule (NCAM), myosin and desmin were studied immunohistochemically. 2. The stem cells showed the morphology of fibroblastic cells. Most of the stem cells showed ATP-induced slow K+ current. T-type Ca2+ current and inward rectifier K+ current were observed in 19% of the stem cells. The stem cells expressed fibronectin, but not NCAM, myosin or desmin. 3. About 2 weeks after the addition of 5-aza-2'-deoxy-cytidine, large multinucleated skeletal muscle-like cells appeared. Most of the induced muscles showed L-type Ca2+ current, responses to acetylcholine, outward K+ current, inward rectifier K+ current and contraction upon depolarizing stimulation. They expressed NCAM, myosin and desmin, but not fibronectin, and showed no ATP response. 4. In some batches (2/14), the induced muscles showed spontaneous twitches, and possessed tetrodotoxin (TTX)-sensitive Na+ current in addition to the currents described above. Furthermore clear striation was observed in some of the twitching muscles under Nomarski optics. 5. To ascertain the properties of cells at the initial step of muscle differentiation, whose differentiation is determined but not yet evident morphologically or electrophysiologically, subcloning was performed from the heterogeneous cells 10 days after induction. Three myogenic clones were obtained, which proliferated at low cell densities but differentiated to muscle with a high incidence at high cell densities, as well as ten non-myogenic clones. 6. Most myogenic clones still showed ATP-induced K+ current and fibronectin. In addition, most of them showed T-type Ca2+ current and inward rectifier K+ current. They had already expressed NCAM. No other properties observed in muscles had yet been expressed. Most cells of the non-myogenic clones showed ATP-induced K+ current and fibronectin. T-type Ca2+ current was also expressed, but not inward rectifier K+ current or NCAM. 7. The properties of the observed ionic currents were studied. The TTX-sensitive Na+ current could be completely blocked by 0.1 microM-TTX. It could be evoked by depolarizing steps to a level above -40 mV, while steady-state inactivation was detectable around -75 mV and reached half by -52 mV. T-type Ca2+ current could be evoked by a depolarizing pulse to a level above -45 mV, with a maximum amplitude around -15 mV.(ABSTRACT TRUNCATED AT 400 WORDS)" @default.
• W2031330996 created "2016-06-24" @default.
• W2031330996 creator A5088049227 @default.
• W2031330996 date "1991-10-01" @default.
• W2031330996 modified "2023-10-03" @default.
• W2031330996 title "Electrophysiological and immunohistochemical analysis of muscle differentiation in a mouse mesodermal stem cell line." @default.
• W2031330996 cites W1575673377 @default.
• W2031330996 cites W1585639196 @default.
• W2031330996 cites W1865658459 @default.
• W2031330996 cites W1964836574 @default.
• W2031330996 cites W1965491546 @default.
• W2031330996 cites W1971287143 @default.
• W2031330996 cites W1972960861 @default.
• W2031330996 cites W1973750924 @default.
• W2031330996 cites W1975424866 @default.
• W2031330996 cites W1992288855 @default.
• W2031330996 cites W2008131022 @default.
• W2031330996 cites W2008905700 @default.
• W2031330996 cites W2015103983 @default.
• W2031330996 cites W2025086061 @default.
• W2031330996 cites W2027931585 @default.
• W2031330996 cites W2034933188 @default.
• W2031330996 cites W2035249816 @default.
• W2031330996 cites W2038659436 @default.
• W2031330996 cites W2041494738 @default.
• W2031330996 cites W2045487678 @default.
• W2031330996 cites W2045636289 @default.
• W2031330996 cites W2045673068 @default.
• W2031330996 cites W2061056399 @default.
• W2031330996 cites W2067375457 @default.
• W2031330996 cites W2073401952 @default.
• W2031330996 cites W2075667471 @default.
• W2031330996 cites W2082470586 @default.
• W2031330996 cites W2092896974 @default.
• W2031330996 cites W2093297626 @default.
• W2031330996 cites W2100906077 @default.
• W2031330996 cites W2103623841 @default.
• W2031330996 cites W2135562504 @default.
• W2031330996 cites W2138027883 @default.
• W2031330996 cites W2140855999 @default.
• W2031330996 cites W2154492664 @default.
• W2031330996 doi "https://doi.org/10.1113/jphysiol.1991.sp018816" @default.
• W2031330996 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1179912" @default.
• W2031330996 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/1665864" @default.
• W2031330996 hasPublicationYear "1991" @default.
• W2031330996 type Work @default.
• W2031330996 sameAs 2031330996 @default.
• W2031330996 citedByCount "16" @default.
• W2031330996 countsByYear W20313309962013 @default.
• W2031330996 countsByYear W20313309962015 @default.
• W2031330996 countsByYear W20313309962021 @default.
• W2031330996 crossrefType "journal-article" @default.
• W2031330996 hasAuthorship W2031330996A5088049227 @default.
• W2031330996 hasBestOaLocation W20313309962 @default.
• W2031330996 hasConcept C122871604 @default.
• W2031330996 hasConcept C147447768 @default.
• W2031330996 hasConcept C1491633281 @default.
• W2031330996 hasConcept C153911025 @default.
• W2031330996 hasConcept C185592680 @default.
• W2031330996 hasConcept C203014093 @default.
• W2031330996 hasConcept C204232928 @default.
• W2031330996 hasConcept C207200792 @default.
• W2031330996 hasConcept C2779072444 @default.
• W2031330996 hasConcept C28328180 @default.
• W2031330996 hasConcept C55493867 @default.
• W2031330996 hasConcept C6997183 @default.
• W2031330996 hasConcept C85789140 @default.
• W2031330996 hasConcept C86803240 @default.
• W2031330996 hasConcept C95444343 @default.
• W2031330996 hasConceptScore W2031330996C122871604 @default.
• W2031330996 hasConceptScore W2031330996C147447768 @default.
• W2031330996 hasConceptScore W2031330996C1491633281 @default.
• W2031330996 hasConceptScore W2031330996C153911025 @default.
• W2031330996 hasConceptScore W2031330996C185592680 @default.
• W2031330996 hasConceptScore W2031330996C203014093 @default.
• W2031330996 hasConceptScore W2031330996C204232928 @default.
• W2031330996 hasConceptScore W2031330996C207200792 @default.
• W2031330996 hasConceptScore W2031330996C2779072444 @default.
• W2031330996 hasConceptScore W2031330996C28328180 @default.
• W2031330996 hasConceptScore W2031330996C55493867 @default.
• W2031330996 hasConceptScore W2031330996C6997183 @default.
• W2031330996 hasConceptScore W2031330996C85789140 @default.
• W2031330996 hasConceptScore W2031330996C86803240 @default.
• W2031330996 hasConceptScore W2031330996C95444343 @default.
• W2031330996 hasIssue "1" @default.
• W2031330996 hasLocation W20313309961 @default.
• W2031330996 hasLocation W20313309962 @default.
• W2031330996 hasLocation W20313309963 @default.
• W2031330996 hasOpenAccess W2031330996 @default.
• W2031330996 hasPrimaryLocation W20313309961 @default.
• W2031330996 hasRelatedWork W1995723030 @default.
• W2031330996 hasRelatedWork W2010859990 @default.
• W2031330996 hasRelatedWork W2035766563 @default.
• W2031330996 hasRelatedWork W2063394580 @default.
• W2031330996 hasRelatedWork W2074829935 @default.
• W2031330996 hasRelatedWork W2088559662 @default.
• W2031330996 hasRelatedWork W2137081819 @default.
• W2031330996 hasRelatedWork W2312575211 @default.

• next