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- W2031396032 abstract "A porous matrix for 3-D cell culture was designed in terms of highly water intrusion property and complete dissolution. The important characteristics of the matrix involve (i) easy cell invasion for 3-D cell culture and (ii) complete degradation and dissolution after tissue regeneration. A bioinspired polymers were prepared by using enantiomeric oligo(L-(D-)lactic acid)macromonomers, 2-methacryroyloxyethyl phosphorylcholine (MPC), and n-butyl methacrylate. Then, the porous matrix was formed by stereocomplexation between L-form and D-form oligomers. The matrix that consisted of aggregation of micro particles and also inter-connecting structure were observed by scanning electron microscopy. The water intrusion property was estimated by measuring the static contact angle of water droplet. The angle gradually decreased within 2 min, and the droplet completely spreaded out due to water intrusion into the matrix. On the other hand, the contact angle did not change on the conventional porous poly(D, L-lactic acid-co-glycolic acid). These results indicated that the bioinspired polymer stereocomplex had a high affinity to the water intrusion. Further, cell invasion into the matrix was carried out by using mouse fibroblast cells. In 5 days, the cells could attach and invade into the matrix. The cell invasion was observed only with the porous matrix. In conclusion, the bioinspired polymer could form porous matrix by stereocomplexation, and the matrix was available to 3-D cell culture." @default.
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- W2031396032 date "2004-01-01" @default.
- W2031396032 modified "2023-09-30" @default.
- W2031396032 title "Artificial Biomembrane Approach for Tissue Regeneration" @default.
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- W2031396032 doi "https://doi.org/10.1380/jsssj.25.23" @default.
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