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- W2031421729 abstract "Fukutome, H., Wada, A., and Kawade, Y. A Photometric Analysis of ultraviolet Photoreaction Kinetics of Transfer RNA. Radiation Res. 37, 599-616 (1969). The kinetics of photoreactions that transfer RNA (tRNA) undergoes on ultraviolet (253.7 m,) irradiation were investigated. For this purpose, a spectrophotometric technique was developed to measure the changes in amount of uracil and cytosine residues in tRNA separately, and applied to yeast tRNA irradiated under various conditions of pH and salt composition. The photoreaction kinetics of uracil residues were found to consist of two phases with different values of apparent cross sections, which are presumed to be due to occurrence of two reactions, dimer formation and hydration. The cross sections of these reactions were shown to become larger at pH below 7, and to decrease when secondary structure develops in tRNA. The photoreaction of cytosine residues was shown to depend considerably on their state of ionization. The photoproduct produced in the uncharged state, presumably a hydrate, was shown to revert almost completely by mild heating, but the one produced in the charged state reverted" @default.
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- W2031421729 date "1969-03-01" @default.
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- W2031421729 title "A photometric analysis of ultraviolet photoreaction kinetics of transfer RNA." @default.
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